Cytokines in Hemopoiesis, Oncology, and AIDS pp 189-195 | Cite as
Effects of Recombinant Cytokines on Clonogenic Cells from Primary Human Tumors
Conference paper
Abstract
Granulocyte-macrophage colony stimulating factor (GM-CSF), granulocyte colony stimulating factor (G-CSF), and interleukin-3 (IL-3) are glycoproteins of known composition involved at different levels in the proliferation and maturation of human hematopoietic precursor cells [1, 2]. The complementary DNAs to their respective mRNAs have been cloned, and recombinant GM-CSF, G-CSF, and IL-3 are currently used in early clinical trials to alleviate chemotherapy-induced bone marrow toxicity [1]. However, recent evidence indicates that these cytokines might also stimulate in vitro growth of human solid tumor cell lines [3, 4]. Also, a variety of human solid tumors has been reported to produce CSFs which might in turn stimulate their own growth by paracrine or autocrine mechanisms [5–8, 23]. The purpose of the present study was to evaluate the effect of GM-CSF, G-CSF, and IL-3 on clonogenic cells from freshly obtained human solid tumor specimens using a capillary soft-agar cloning system.
Keywords
Colony Formation Granulocyte Colony Stimulate Factor Clonogenic Cell Recombinant Cytokine Primary Human Tumor
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© Springer-Verlag, Berlin Heidelberg 1990