Conformational and Shape Changes Associated with cAMP- Dependent Protein Kinase

  • Friedrich W. Herberg
  • Susan S. Taylor
Conference paper
Part of the NATO ASI Series book series (volume 56)


Cyclic-AMP dependent protein kinase (cAPK) was one the first discovered protein kinases (Walsh et al., 1968) and is one of the best understood biochemically of the protein kinases (Taylor et al., 1990). cAPK is composed of both regulatory (R) and catalytic (C) subunits. In the absence of cAMP the subunits associate with a high affinity to form an inactive tetrameric (R2C2) holoenzyme. cAMP binds to the R-subunit promoting the dissociation of the complex into a dimeric R-subunit and two monomeric active C-subunits which transfer the γ-phosphate of ATP to a serine or threonine of a peptide or protein substrate (Beebe and Corbin, 1986).


Catalytic Subunit Protein Kinase Inhibitor Dependent Protein Kinase Mammalian Enzyme cAMP Binding 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.


Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.


  1. G. K. Ackers (1975) Molecular Sieve Methods of Analysis In: The Proteins, 3rd Edition. Vol. 1 ( Neurath, H., and Hill, R. eds. ): Academic Press New YorkGoogle Scholar
  2. S. J. Beebe and J. D. Corbin (1986) Cyclic Nucleotide-Dependent Protein Kinases. The Enzymes: Control by Phosphorylation Part A,Vol. XVII eds. P.D. Boyer and E. G. Krebs Academic Press, Inc. New York.Google Scholar
  3. S. A Carr,.Biemann, K.Shuji, S.Parmalee, D. C. Titani, K. (1982) n-Tetradecanoyl in the NH2 Terminal Blocking Group of the Catalytic Subunit of the Cyclic AMP- Dependent Protein Kinase from Bovine Cardiac Muscle. Proc. Natl. Acad. Sci. USA 79: 6128–6131PubMedCrossRefGoogle Scholar
  4. P.F. Cook,.Neville, M. E.Vrana, K. E.Hartl, F. T.Roskoski, Jr., R. (1982) Adenosine Cyclic 3′5′-Monophosphate Dependent Protein Kinase: Kinetic Mechanism for the Bovine Skeletal Muscle Catalytic Subunit. Biochemistry 21: 5794–5799PubMedCrossRefGoogle Scholar
  5. R. J.Duronio, Jackson-Machelski, E.Heuckeroth, R. O.Olins, P.Devine, C. S.Yonemoto, W.Slice, L. W.Taylor, S. S. Gordon, J. (1990)1.Protein N- myristoylation in Escherichia coli: Reconstitution of a Eukaryotic Protein Modification in BacteriaProc. Natl. Acad. Sci. USA 87: 1506–1510CrossRefGoogle Scholar
  6. T.A. Kunkel (1985) Rapid and efficient site-specific mutagenesis without phenotypic selection Proc. Natl. Acad. Sci. USA 82: 488–492PubMedCrossRefGoogle Scholar
  7. J. Parello, P. A. Timmins, J. M. Sowadski and S. S. Taylor (1990) Major Conformational Changes in the Catalytic Subunit of cAMP-Dependent Protein Kinase Induced by the Binding of a Peptide Inhibitor: A Small-Angle Neutron Scattering Study in Solution. J. Mol. Biol, submittedGoogle Scholar
  8. J. Reed,.Kinzel, V Kemp, B.E. Cheung, H.-C Walsh, D.A. (1985) Circular Dichroic Evidence for an Ordered Sequence of Ligand/Binding Site Interactions in the Catalytic Reaction of the cAMP-Dependent Protein Kinase Biochem. 24: 2967–2973CrossRefGoogle Scholar
  9. L. D. Saraswat, M. Filutowics and S. S. Taylor (1986) Expression of the Type I Regulatory Subunit of cAMP-Dependent Protein Kinase in Escherichia coli. J. Biol. Chem. 261: 11091–11096PubMedGoogle Scholar
  10. L. W. Slice and S. S. Taylor (1989) Expression of the Catalytic Subunit of cAMP- dependent Protein Kinase In Escherichia coli. J. Biol. Chem. 264: 20940–20946PubMedGoogle Scholar
  11. S. S. Taylor, J. A. Buechler and Y. Yonemoto (1990) cAMP-dependent Protein Kinase: Framework for a Diverse Family of Regulatory Enzymes. Ann. Rev. Biochem. 59: 971–1005PubMedCrossRefGoogle Scholar
  12. S. M. Van Patten, W. H. Fletcher and D. A. Walsh (1986) The Inhibitor Protein of the cAMP-Dependent Protein Kinase-Catalytic Subunit Interaction. J. Biol. Chem. 261: 5514–5523PubMedGoogle Scholar
  13. D. A. Walsh, K. L. Angelos, S. M. Van Patten, D. B. Glass and L. P. Garetto (1990) The Inhibitor Protein of the cAMP-Dependent Protein Kinase. CRC Crit. Rev. Biochem. 43–84Google Scholar
  14. I. T.Weber, Steitz, T. A.Bubis, J.Taylor, S. S. (1987) Predicted Structures of the cAMP Binding Domains of the Type I and II Regulatory Subunits of cAMP- Dependent Protein Kinase Biochem 26: 343–351CrossRefGoogle Scholar
  15. D. A. Walsh, J. P. Perkins and E. G. Krebs (1968) An Adenosine 3′,5′-Mono- Phosphate-Dependent Protein Kinase from Rabbit Skeletal Muscle. J. Biol. Chem. 243: 3763–3765PubMedGoogle Scholar
  16. M.N. Zoller, Kerlavage, A. R.Taylor, S. S. (1979) Structural Comparisons of cAMP- Dependent Protein Kinases I and II from Porcine Skeletal Muscle J. Biol. Chem. 254: 2408–2412PubMedGoogle Scholar

Copyright information

© Springer-Verlag Berlin Heidelberg 1991

Authors and Affiliations

  • Friedrich W. Herberg
    • 1
  • Susan S. Taylor
    • 1
  1. 1.Department of ChemistryUniversity of California, San DiegoLa JollaUSA

Personalised recommendations