Dissection of the Light-Responsive Elements of Pea RBCS3A

  • P. M. Gilmartin
  • J. Memelink
  • N.-H. Chua
Conference paper
Part of the NATO ASI Series book series (volume 50)

Abstract

Genes encoding the small subunit of ribulose-l/5-bisphosphate carboxylase-oxygenase (rbcs) and the chlorophyll a/b binding proteins (cab) have been the focus of many studies on the organization and regulation of plant nuclear genes, rbcs and cab genes are typically light-responsive. In etiolated and dark-adapted tissue transcript abundance is low; following illumination of the plant rbcs and cab transcript abundance increases dramatically (see Tobin & Silverthorne 1985; Kuhlemeier et al., 1987a; Jenkins, 1988). There are however exceptions to this rule in which the transcripts of specific rbcs or cab genes are also present in the dark (Manzara & Gruissem, 1988; Sullivan et al., 1989). Light-responsive genes that exhibit a reciprocal pattern of expression, namely, high transcript levels in the dark and low levels in the light, have also been characterized (Batschauer & Apel, 1984; Lissemore & Quail, 1988; kay et al., 1989; Darrah et al., 1990; Tsai & Coruzzi, 1990; Kay, this volume).

Keywords

Upstream Sequence Chloramphenicol Acetyl Transferase Heat Shock Element Transgenic Petunia Filter Binding Assay 
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Copyright information

© Springer-Verlag Berlin Heidelberg 1991

Authors and Affiliations

  • P. M. Gilmartin
    • 1
  • J. Memelink
    • 1
  • N.-H. Chua
    • 1
  1. 1.Laboratory of Plant Molecular BiologyThe Rockefeller UniversityNew YorkUSA

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