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Purification of an Odorant Binding Protein from Human Nasal Mucosa

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Chemosensory Information Processing

Part of the book series: NATO ASI Series ((ASIH,volume 39))

Abstract

The nasal mucosa of several mammals contains a protein that binds several odorants with dissociation constants in the micromolar range and is not found anywhere else in the body. This protein, that is very abundant, accounting for up to 1% of total soluble protein in the nasal mucosa, has been first identified and purified from the cow (Pelosi et al.,1982; Bignetti et al.,1985a; Pevsner et al.,1985) and fully characterized both in terms of structure and of binding specificity (Bignetti et al.,1985b, 1987; Cavaggioni et al.,1987; Pelosi and Tirindelli, 1988; Pevsner et al., 1986, 1988b; Topazzini et al., 1985).

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References

  • Avanzini,F., Bignetti,E., Bordi,C., Carfagna,G., Cavaggioni,A., Ferrari,G., Sorbi,R.T. and Tirindelli,R. 1987. Immunocytochemical localization of pyrazine-binding protein in the cow nasal mucosa. Cell Tissue Res., 247: 461

    Article  PubMed  CAS  Google Scholar 

  • Baldaccini,N.E., Gagliardo,A., Pelosi,P. and Topazzini,A. 1986. Occurrence of a pyrazine binding protein in the nasal mucosa of some vertebrates. Comp. Biochem. Physiol., 84B: 249

    CAS  Google Scholar 

  • Bignetti,E., Cavaggioni,A., Pelosi,P., Persaud,K.C., Sorbi,R.T. and Tirindelli,R 1985a. Purification and characterization of an odorant binding protein from cow nasal mucosa. Eur. J. Biochem. 149: 227

    Article  CAS  Google Scholar 

  • Bignetti,E., Tirindelli,R., Rossi,G.L., Bolognesi,M., Coda,A. and Gatti,G. 1985b. Crystallization of an odorant binding protein from cow nasal mucosa. J. Mol. Biol. 186: 211

    Article  CAS  Google Scholar 

  • Bignetti,E., Damiani,G., De Negri,P., Ramoni,R., Avanzini,F., Ferrari,G. and Rossi,G.L. 1987a. Specificity of an immunoaffinity column for odorant-binding protein from bovine nasal mucosa. Chem. Senses 12: 601

    Article  CAS  Google Scholar 

  • Cavaggioni,A., Sorbi,R.T., Keen,J.N., Pappin,D.J.C. and Findlay,J.B.C. 1987. Homology between the pyrazine binding protein from nasal mucosa and major urinary proteins. FEBS Lett. 212: 225

    Article  PubMed  CAS  Google Scholar 

  • Pelosi,P., Baldaccini,N.E. and Pisanelli,A.M. 1982. Identification of a specific olfactory receptor for 2-isobutyl-3-methoxypyrazine. Biochem. J. 201: 245

    PubMed  CAS  Google Scholar 

  • Pelosi P. and Tirindelli R. 1988. Structure/activity studies and characterization of the pyrazine binding protein. Int. Conf. on Chemosensory Transduction: “Receptor and transduction mechanisms in taste and olfaction”, Philadelphia ( USA ), March 1988

    Google Scholar 

  • Pevsner,J., Trifiletti,R.R., Strittmatter,S.M. and Snyder,S.H. 1985. Isolation and characterization of an olfactory receptor protein for odorant pyrazines. Proc. Natl. Acad. Sci. USA 82: 3050

    Article  PubMed  CAS  Google Scholar 

  • Pevsner,J., Sklar,P.B. and Snyder,S.H. 1986. Odorant-binding protein: localization to nasal glands and secretion. Proc. Natl. Acad. Sci. USA 83: 4942

    Article  PubMed  CAS  Google Scholar 

  • Pevsner,J., Reed,R.R., Feinstein,P.G. and Snyder,S.H. 1988a. Molecular cloning of odorant-binding protein: member of a ligand carrier family Science 241: 336

    Article  CAS  Google Scholar 

  • Pevsner,J., Hwang,P.M., Sklar,P.B.,Venable,J.C. and Snyder,S.H. 1988b. Odorant-binding protein and its mRNA are localized to lateral nasal gland implying a carrier function. Proc. Natl. Acad. Sci. USA 85: 1

    Article  Google Scholar 

  • Topazzini,A., Pelosi,P., Pasqualetto,P.L. and Baldaccini,N.E. 1985. Specificity of a pyrazine binding protein from cow olfactory mucosa. Chem. Senses 10: 45

    Article  CAS  Google Scholar 

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© 1990 Springer-Verlag Berlin Heidelberg

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Pelosi, P., Maremmani, C., Muratorio, A. (1990). Purification of an Odorant Binding Protein from Human Nasal Mucosa. In: Schild, D. (eds) Chemosensory Information Processing. NATO ASI Series, vol 39. Springer, Berlin, Heidelberg. https://doi.org/10.1007/978-3-642-75127-1_9

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  • DOI: https://doi.org/10.1007/978-3-642-75127-1_9

  • Publisher Name: Springer, Berlin, Heidelberg

  • Print ISBN: 978-3-642-75129-5

  • Online ISBN: 978-3-642-75127-1

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