Hormonal Regulation of Phospholipases

  • J. H. Exton
Conference paper
Part of the 40. Colloquium der Gesellschaft für Biologische Chemie 6.– 8. April 1989 in Mosbach/Baden book series (MOSBACH, volume 40)

Abstract

It is now widely accepted that many hormones, neurotransmitters, and related agonists exert some of their biological effects by breaking down phosphatidylinositol 4,5-biphosphate (PIP2) a minor phospholipid in the plasma membrane of their target cells. This produces two signaling molecules: inositol trisphosphate (IP3) which releases intracellular Ca2+, and diacylglycerol (DAG) which activates protein kinase C (Berridge 1987). There is much evidence that the phospholipase C that catalyzes the breakdown of PIP2 is regulated by a guanine nucleotide-binding regulatory protein (G-protein) which is activated when the relevant plasma membrane receptors are occupied by agonists (Exton 1988a).

Keywords

Phosphatidic Acid Phorbol Ester Phosphatidic Acid Liver Plasma Membrane Inositol Trisphosphate 
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References

  1. Agwu, D.E., McPhail, L.D., Chabot, M.C., Daniel, L.W., Wykle, R.L. & McCall, C.E. (1989) Choline-linked phosphogylcerides: a source of phosphatidic acid and diglycerides in stimulated neutrophils. J. Biol. Chem. 264: 1405–1413PubMedGoogle Scholar
  2. Augert, G., Blackmore, P.F. & Exton, J.H. (1989a) Changes in the concentration and fatty acid composition of phosphoinositides induced by hormones in hepatocytes. J. Biol. Chem. 264: 2574–2580PubMedGoogle Scholar
  3. Augert, G., Bocckino, S.B., Blackmore, P.F. & Exton, J.H. (1989b) Hormonal stimulation of diacylglycerol formation in hepatocytes. Evidence for phosphatidylcholine breakdown. J. Biol. Chem. (in press) Berridge, M.J. (1987) Inositol trisphosphate and diacylglycerol: two interacting second messengers. Annu Rev. Biochem. 56:159–193Google Scholar
  4. Besterman, J.M., Duronio, V. & Cuatrecasas, P. (1986) Rapid formation of diacylglycerol from phospha-tidylcholine: a pathway for generation of a second messenger. Proc. Natl. Acad. Sci. USA 83: 6785–6789PubMedCrossRefGoogle Scholar
  5. Bocckino, S.B., Blackmore, P.F. & Exton, J.H. (1985) Stimulation of 1,2-diacylglycerol accumulation in hepatocytes by vasopressin, epinephrine and angiotensin II. J. Biol. Chem. 260: 14201–14207PubMedGoogle Scholar
  6. Bocckino, S.B., Blackmore, P.F., Wilson, P.B. & Exton, J.H. (1987) Phosphatidate accumulation in hormone-treated hepatocytes via a phospholipase D mechanism. J. Biol. Chem. 262: 15309–15315PubMedGoogle Scholar
  7. Cabot, M.C., Welsh, C.J., Zhang, Z.-C., Cao, H.-T., Chabbott, H. & Lebowitz, M. (1988a) Vasopressin, phorbol diesters and serum elicit choline glycerophospholipid hydrolysis and diacylglycerol formation in nontransformed cells: transformed derivatives do not respond. Biochim. Biophys. Acta 959: 46–57Google Scholar
  8. Cabot, M.C., Welsh, C.J., Cao, H.-T. & Chabbott, H. (1988b) The phosphatidylcholine pathway of diacylglycerol formation stimulated by phorbol diesters occurs via phospholipase D activation. FEBS Lett. 233: 153–157PubMedCrossRefGoogle Scholar
  9. Daniel, L.W., Waite, M. & Wykle, R.L. (1986) A novel mechanism of diglyceride formation: 12–0tetradecanoylphorbol-13-acetate stimulates the cyclic breakdown and resynthesis of phosphatidylcholine. J. Biol. Chem. 261: 9128–9132Google Scholar
  10. Exton, J.H. (1988a) The roles of calcium and phosphoinositides in the mechanisms of a, -adrenergic and other agonists. Rev. Physiol. Biochem. Pharmacol. 111: 118–224Google Scholar
  11. Exton, J.H. (1988b) Mechanisms of action of calcium-mobilizing agonists: some variations on a young theme. FASEB J. 2: 2670–2676PubMedGoogle Scholar
  12. Fitzgerald, T.J., Uhing, R.J. & Exton, J.H. (1986) Solubilization of the vasopressin receptor from rat liver membranes. J. Biol. Chem. 261: 16871–16877PubMedGoogle Scholar
  13. Guy, G.R. & Murray, A.W. (1982) Tumor promoter stimulation of phosphatidylcholine turnover in HeLa cells. Cancer Res. 42: 1980–1985PubMedGoogle Scholar
  14. Irving, H.R. & Exton, J.H. (1987) Phosphatidylcholine breakdown in rat liver plasma membranes. J. Biol. Chem. 262: 3440–3443PubMedGoogle Scholar
  15. Kolesnick, R.N. & Paley, A.E. (1987) 1,2-Diacylglycerols and phorbol esters stimulate phosphatidylcholine metabolism in GH, pituitary cells: evidence for separate mechanisms of action. J. Biol. Chem. 262: 9204–9210PubMedGoogle Scholar
  16. Liscovitch, M., Blusztajn, J.K., Freese, A. & Wurtman, R.J. (1987) Stimulation of choline release from NG 108–15 cells by 12-O-tetradecanoylphorbol 13-acetate. Biochem. J. 241: 81–86PubMedGoogle Scholar
  17. Lynch, C.J., Prpic, V., Blackmore, P.F. & Exton, J.H. (1986) Effect of islet-activating pertussis toxin on the binding characteristics of Ca-mobilizing hormones and on agonist activation of phosphorylase in hepatocytes. Mol. Pharmacol. 29: 196–203PubMedGoogle Scholar
  18. Martin, T.W. & Michaelis, K.C. (1988) Bradykinin stimulates phosphodiesteratic cleavage of phospha-tidylcholine in cultured endothelial cells. Biochem. Biophys. Res. Commun. 157: 1271–1279CrossRefGoogle Scholar
  19. Moolenaar, W.H., Kruijer, W., Tilly, B.C., Verlaan, I., Bierman, A.J. & deLaat, S.W. (1986) Growth factor-like action of phosphatidic acid. Nature (London) 310:644–649Google Scholar
  20. Moolenaar, W.H., Kruijer, W., Tilly, B.C., Verlaan, I., Bierman, A.J. & deLaat, S.W. (1986) Growth factor-like action of phosphatidic acid. Nature (London) 310: 644–649Google Scholar
  21. Mufson, R.A., Okin, E. & Weinstein, I.B. (1981) Phorbol esters stimulate the rapid release of choline from prelabelled cells. Carcinogenesis 2: 1095–1102PubMedCrossRefGoogle Scholar
  22. Muir, J.G. & Murray, A.W. (1987) Bombesin and phorbol ester stimulate phosphatidylcholine hydrolysis by phospholipase C: evidence for a role of protein kinase C. J. Cell. Physiol. 130: 382–391PubMedCrossRefGoogle Scholar
  23. Pai, J.-K., Siegel, M.I., Egan, R.W. & Billah, M.M. (1988a) Activation of phospholipase D by chemotactic peptide in HL-60 granulocytes. Biochem. Biophys. Res. Commun. 150: 355–364CrossRefGoogle Scholar
  24. Pai, J.-K., Siegel, M.I., Egan, R.W. & Billah, M.M. (1988b) Phospholipase D catalyzes phospholipid metabolism in chemotactic peptide-stimulated HL-60 granulocytes. J. Biol. Chem. 263: 12472–12477Google Scholar
  25. Pickford, L.B., Polverino, A.J. & Barritt, G.J. (1987) Evidence from studies employing radioactively labelled fatty acids that the stimulation of flux through the diacylglycerol pool is an early action ofvasopressin on hepatocytes. Biochem. J. (1987) 245: 211–216PubMedGoogle Scholar
  26. Polverino, A.J. & Barritt, G.J. (1988) On the source of the vasopressin-induced increases in diacylglycerol in hepatocytes. Biochim. Biophys. Acta 970: 75–75PubMedCrossRefGoogle Scholar
  27. Ryu, S.H., Cho, K.S., Lee, K.-Y., Suh, P.-G. & Rhee, S.G. (1987a) Purification and characterization of two immunologically distinct phosphoinositide-specific phospholipases C from bovine brain. J. Biol. Chem. 262: 12511–12518Google Scholar
  28. Ryu, S.H., Suh, P.-G., Cho, K.S., Lee, K.-Y. & Rhee, S.G. (1987b) Bovine brain cytosol contains three immunologically distinct forms of inositolphospholipid-specific phospholipase C. Proc. Natl. Acad Sci. USA 84: 6649–6653CrossRefGoogle Scholar
  29. Slivka, S.R., Meier, K.E. & Insel, P.A. (1988) a,-Adrenergic receptors promote phosphatidylcholine hydrolysis in MDCK-D1 cells: a mechanism for rapid activation of protein kinase C. J. Biol. Chem. 263: 12242–12246PubMedGoogle Scholar
  30. Takuwa, N., Takuwa, Y. & Rasmussen, H. (1987) A tumour promoter, 12-O-tetradecanoylphorbol 13-acetate, increases cellular 1,2-diacylglycerol content through a mechanism other than phosphoinositide hydrolysis in Swiss-mouse 3T3 fibroblasts. Biochem. J. 243:647–653Google Scholar
  31. Takuwa, N., Takuwa, Y. & Rasmussen, H. (1987) A tumour promoter, 12-O-tetradecanoylphorbol 13-acetate, increases cellular 1,2-diacylglycerol content through a mechanism other than phosphoinositide hydrolysis in Swiss-mouse 3T3 fibroblasts. Biochem. J. 243: 647–653PubMedGoogle Scholar
  32. Taylor, S.J. & Exton, J.H. (1987) Guanine-nucleotide and hormone regulation of polyphosphoinositide phospholipase C activity of rat liver plasma membranes. Biochem. J. 248: 791–799PubMedGoogle Scholar
  33. Truett, A.P., III, Verghese, M.W., Dillon, S.B. & Snyderman, R. (1988) Calcium influx stimulates a second pathway for sustained diacylglycerol production in leukocytes activated by chemoattractants. Proc. Natl. Acad. Sci. USA 85: 1549–1553CrossRefGoogle Scholar
  34. Uhing, R.J., Prpic, V., Jiang, H. & Exton, J.H. (1986) Hormone-stimulated polyphosphoinositide breakdown in rat liver plasma membranes. J. Biol. Chem. 261: 2140–2146PubMedGoogle Scholar
  35. Yu, C.-L., Tsai, M.-H. & Stacey, D.W. (1988) Cellular rat activity and phospholipid metabolism. Cell 52: 63–71PubMedCrossRefGoogle Scholar

Copyright information

© Springer-Verlag Berlin Heidelberg 1989

Authors and Affiliations

  • J. H. Exton
    • 1
  1. 1.Howard Hughes Medical Institute and Departments of Molecular Physiology and Biophysics, and PharmacologyVanderbilt University School of MedicineNashvilleUSA

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