Flax (Linum usitatissimum L.): In Vitro Studies

  • A. McHughen
Part of the Biotechnology in Agriculture and Forestry book series (AGRICULTURE, volume 10)


The genus Linum contains one species of economic importance as a crop (usitatissimum), although some other species have limited value as ornamentals. Linum usitatissimum has been cultivated by man for thousands of years; its importance even in ancient times is evidenced by its importation by the Egyptians over 6000 years ago (Trump 1980) and it has been found in remains of prehistoric settlements. The plants were useful as a source of fibre for weaving, oil from the seeds and feed from the meal, hence the species name, usitatissimum — meaning “most useful”. In addition to being versatile, the plant has been adapted to grow in many parts of the world, and is cropped on all arable continents.


Somaclonal Variation Linum Usitatissimum Aster Yellow Flax Plant Flax Genome 
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  1. Barakat MN, Cocking EC (1983) Plant regeneration from protoplast-derived tissue of Linum usitatissimum L. (flax). Plant Cell Rep 2: 314–317CrossRefGoogle Scholar
  2. Barakat MN, Cocking EC (1984) An assessment of the cultural capabilities of protoplasts of some wild species of Linum. Plant Cell Rep 4: 164–167CrossRefGoogle Scholar
  3. Bari G, Godward MBE (1970) Interspecific crosses in Linum. Euphytica 19: 443–446CrossRefGoogle Scholar
  4. Basiran N, Armitage P, Scott R, Draper J (1987) Genetic transformation of flax (Linum usitatissimum) by Agrobacterium tumefaciens: Regeneration of transformed shoots via a callus phase. Plant Cell Rep 6: 396–399Google Scholar
  5. Chaleff RS (1983) Isolation of agronomically useful mutants from plant cell cultures. Science 219: 676–682PubMedCrossRefGoogle Scholar
  6. Chlyah H, Squalli-Khalil M, Chlyah A (1980) Dimorphism in bud regeneration in flax CLinum usitatissimum). Can J Bot 58: 637–641CrossRefGoogle Scholar
  7. Ciamporova M, Pretova A (1981) Ultrastructural changes of plastids in flax embryos cultivated in vitro. New Phytol 87: 473–479CrossRefGoogle Scholar
  8. Cullis CA (1977) Molecular aspects of the environmental induction of heritable changes in flax. Heredity 38: 129–154CrossRefGoogle Scholar
  9. Cullis CA (1981) DNA sequence organisation in the flax genome. Biochim Biophys Acta 652: 1–15PubMedGoogle Scholar
  10. Cullis CA (1984) The flax genome - its plasticity and response to stress. (Abstr) Heredity 53: 576Google Scholar
  11. Cullis CA, Cleary W (1986) DNA variation in flax tissue culture. Can J Plant Sci 28: 247–251Google Scholar
  12. Dillman AC (1936) Improvement in flax. Yearbook of Agriculture. USDA, Washington, pp 745–784Google Scholar
  13. Durrant A (1958) Environmental conditioning in flax. Nature (Lond) 181: 928–929CrossRefGoogle Scholar
  14. Dybing CD, Lay C (1981) Flax. In: McClure TA, Lipinsky ES (eds) Dybing CD, Lay C, pp 71–85 FAOGoogle Scholar
  15. (1982).
    Production Yearbook, FAO, RomeGoogle Scholar
  16. Flor HH (1982) Plastic vs. stable flax lines. In: Proc 49th Ann Flax Institute of the US, Fargo, pp 74–93Google Scholar
  17. Gamborg OL, Shyluk JP (1976) Tissue culture, protoplasts and morphogenesis in flax. Bot Gaz 137: 301–306CrossRefGoogle Scholar
  18. Gamborg OL, Miller RA, Ojima K (1968) Nutrient requirements of suspension cultures of soybean root cells. Exp Cell Res 21: 359–368Google Scholar
  19. Gill KS, Yermanos DM (1967) Cytogenetic studies on the genus Linum. 1. Hybrids among taxa with 15 as the haploid number. Crop Sci 7: 623–627CrossRefGoogle Scholar
  20. Green AG (1986) A mutant genotype of flax (Linum usitatissimum L.) containing very low levels of linolenic acid in its seed oil. Can J Plant Sci 66: 499–503CrossRefGoogle Scholar
  21. Haughn G, Smith J, Mazur B, Somerville C (1988) Transformation with a mutant Arabidopsis acetolactate synthase gene renders tobacco resistant to sulfonylurea herbicides. Mol Gen Genet 211: 266–271CrossRefGoogle Scholar
  22. Hepburn AG, Clarke LE, Blundy KS, White J (1983) Nopaline Ti-plasmid, pTiT37, T-DNA insertions into a flax genome. Molec Appl Genet 2: 211–224Google Scholar
  23. Hoes JA (1975) Diseases of flax in Western Canada. In: Harapiuk JT (ed) Oilseed and pulse crops in Western Canada. Western Co-operative Fertilizers, Calgary, pp 415–424Google Scholar
  24. Ibrahim RK (1971) Media for growth of flax tissue culture. Can J Bot 49: 295–298CrossRefGoogle Scholar
  25. Ibrahim RK, Phan CT (1978) Phenolic synthesis in relation to chloroplast ultrastructure in flax callus and cell suspension cultures. Biochem Physiol Pflanz 172: 199–212Google Scholar
  26. Jordan MC, McHughen A (1987) Selection for chlorsulfuron resistance in flax ( Linum usitatissimum) cell cultures. J Plant Physiol 131: 333–338Google Scholar
  27. Jordan MC, McHughen A ( 1988 a). Glyphosate tolerant flax plants from Agrobacterium-medizted gene transfer. Plant Cell Rep 7: 281–284Google Scholar
  28. Jordan MC, McHughen A ( 1988 b). Transformed callus does not necessarily regenerate transformed shoots. Plant Cell Rep 7: 285–287Google Scholar
  29. Kenaschuk EO (1975). Flax breeding and genetics. In: Harapiuk JT (ed) Oilseed and pulse crops in Western Canada. Western-Cooperative Fertilizers, Calgary, pp 203–221Google Scholar
  30. Lane WD (1979) Influence of growth regulators on root and shoot initiation from flax meristem-tips and hypocotyls in vitro. Physiol Plant 45: 260–264CrossRefGoogle Scholar
  31. Larkin PJ, Scowcroft WR (1981) Somaclonal variation - a novel source of variability from cell cultures for plant improvement. Theor Appl Genet 60: 197–214CrossRefGoogle Scholar
  32. Ling HQ, Binding H (1987) Plant regeneration from protoplasts in Linum. Plant Breed 98: 312–317CrossRefGoogle Scholar
  33. Link GKK, Eggers V (1946) Mode, site and time of initiation of hypocotyledonary bud primordia in Linum usitatissimum L. Bot Gaz 107: 441–454CrossRefGoogle Scholar
  34. Linsmaier EM, Skoog F (1965) Organic growth factor requirements of tobacco tissue cultures. Physiol Plant 18: 100–127CrossRefGoogle Scholar
  35. Martin JH, Leonard WH (1949) Principles of field crop production. MacMillan, New YorkGoogle Scholar
  36. Mathews VH, Narayanaswamy S (1976) Phytohormone control of regeneration in cultured tissues of flax. Z Pflanzenphysiol 80: 436–442Google Scholar
  37. McHughen A (1984) Salt-tolerant flax plants regenerated from cells surviving a highly saline culture medium. In: Symp Genetic manipulation in Crops. 22–26 October, 1984, Beijing, ChinaGoogle Scholar
  38. McHughen A (1987) Salt tolerance through increased vigor in a flax line (STS-II) selected for salt tolerance in vitro. Theor Appl Gen 74: 727–732CrossRefGoogle Scholar
  39. McHughen A, Rowland G (1988) Canada’s first somaclonal variant crop cultivar. 2nd Canadian Plant Tissue Culture Genetic Engineering Workshop. May-June, 1988, Ottawa, Canada, p 43Google Scholar
  40. McHughen A, Swartz M (1984) A tissue-culture derived salt-tolerant line of flax ( Linum usitatissimum ). J Plant Physiol 117: 109–117Google Scholar
  41. McHughen A, Browne R, Kneeshaw D, Jordan M (1986) Ti-mediated transformation and regeneration of flax plants in vitro. 6th Int Congr IAPTC; Minneapolis, USA, p 130Google Scholar
  42. Murashige T, Skoog F (1962) A revised medium for rapid growth and bioassays with tobacco tissue cultures. Physiol Plant 15: 473–497CrossRefGoogle Scholar
  43. Murray BE, Handyside RJ, Keller WA (1977) In vitro regeneration of shoots on stem explants of haploid and diploid flax (.Linum usitatissimum). Can J Genet Cytol 19: 177–186Google Scholar
  44. Norstog K (1973) New synthetic medium for the culture of premature barley embryos. In Vitro 8: 307–308Google Scholar
  45. Prefova A, Williams EG (1986) Direct somatic embryogenesis from immature zygotic embryos of flax ( Linum usitatissimum L. ). J Plant Physiol 126: 155–161Google Scholar
  46. Quatrano R (1968) Freeze-preservation of cultured flax cells utilizing dimethyl sulfoxide. Plant Physiol 43: 2057–2061PubMedCrossRefGoogle Scholar
  47. Rybczynski J J (1975) Callus formation and organogenesis of mature cotyledons of Linum usitatissimum L. var. szokijskij in vitro culture. Genet Pol 16: 1–12Google Scholar
  48. Seetharam A (1972) Interspecific hybridization in Linum. Euphytica 212: 489–495CrossRefGoogle Scholar
  49. Singh BD, Harvey BL, Kao KN, Miller RA (1975) Karyotypic changes and selection pressure in Haplopappus gracilis suspension cultures. Can J Genet Cytol 17: 109–116Google Scholar
  50. Sun H, Fu W (1981) Induction of pollen plants in flax ( Linum usitatissimum) and preliminary observations on performance of their progenies. Acta Genet Sin 8: 369–374Google Scholar
  51. Tammes T (1928) The genetics of the genus Linum. Bibliogr Genet 4: 1–36Google Scholar
  52. Trump DH (1980) The prehistory of the Mediterranean. Yale Univ Press, New Haven, LondonGoogle Scholar
  53. White PR (1963) The cultivation of animal and plant cells. 2nd ed. Ronald, New YorkGoogle Scholar

Copyright information

© Springer-Verlag Berlin Heidelberg 1990

Authors and Affiliations

  • A. McHughen
    • 1
  1. 1.Crop Development CentreUniversity of SaskatchewanSaskatoonCanada

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