Methods for the Isolation of Higher Plant Vacuoles

  • A. Pugin
  • A. Kurkdjian
  • D. Le Quôc
  • K. Le Quôc
Chapter

Abstract

The central vacuole of the plant cell is a large membrane-bound organelle which occupies up to 90 % of the total cell volume. There is evidence indicating that the vacuole participates in preservation of cytoplasmic homeostasis, cell turgor, accumulation of ions and metabolites (sugars, aminoacids, organic acids) ; secondary metabolites such as alkaloids are also stored in this compartment (for reviews, see Matile, 1978 ; Leigh, 1983 ; Boller and Wiemken, 1986).

Keywords

Osmotic Shock Fluorescein Diacetate Conical Centrifuge Tube Start Reaction Plant Vacuole 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.
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Copyright information

© Springer-Verlag Berlin Heidelberg 1988

Authors and Affiliations

  • A. Pugin
  • A. Kurkdjian
  • D. Le Quôc
  • K. Le Quôc

There are no affiliations available

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