Restricted Neutralization of Divergent HTLV-III/LAV Isolates by Antibodies to the Major Envelope Glycoprotein

  • Th. J. Matthews
  • A. J. Langlois
  • W. G. Robey
  • N. T. Chang
  • R. C. Gallo
  • P. J. Fischinger
  • D. P. Bolognesi
Conference paper
Part of the Haematology and Blood Transfusion / Hämatologie und Bluttransfusion book series (HAEMATOLOGY, volume 31)

Summary

By analogy to other retroviruses, the major envelope glycoprotein — gp120 — of HTLV-III/LAV is a probable target for neutralizing antibody. This antigen has been purified from H9 cells chronically infected with the HTLV-IIIB prototype strain. Several goats immunized with the gp120 produced antibodies that neutralized infection of H9 by the homologous virus isolate. These same sera failed to neutralize the divergent HTLV-IIIRF isolate. Individuals infected with HTLV-III/LAV commonly develop antibodies to gp120 which could be isolated using the gp120 antigen coupled to an immunoadsorbent resin. The antibody fraction that bound tightly to such a resin was found to neutralize the IIIB but not the RF isolate in a fashion similar to that of the goat anti-gp120 sera. However, the nonbinding fraction (effluent) from the resin also contained neutralizing activity which was able to block infection by both virus isolates with similar efficacy. Human antibodies to the other virus envelope gene product, the transmem-brane gp41, were also affinity-purified utilizing the recombinant peptide 121, but they failed to influence infection by either virus isolate.

Keywords

Neutralize Activity Recombinant Peptide Goat Seron Antibody Fraction Divergent Isolate 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.
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References

  1. 1.
    Gonda MA, Wong-Staal F, Gallo RC, Clements JT, Narayan O, Gilden RV (1985) Science 227:173–177PubMedCrossRefGoogle Scholar
  2. 2.
    Gallo RC, Salahuddin SZ, Popovic M, Shearer GM, Kaplan M, Haynes BF, Palker TJ et al. (1984) Science 224:500–503PubMedCrossRefGoogle Scholar
  3. 3.
    Shaw GM, Hahn B, Arya SK, Groopman JE, Gallo RC, Wong-Staal F (1984) Science 226:1165–1171PubMedCrossRefGoogle Scholar
  4. 4.
    Sarngadharan MG, Popovic M, Bruch L, Schupbach J, Gallo RC (1984) Science 224:506–508PubMedCrossRefGoogle Scholar
  5. 5.
    Safai B, Groopman JE, Popovic M, Schupbach J, Sarngadharan MG, Arnett K, Sliski NA, Gallo RC (1984) Lancet 1:1438–1440PubMedCrossRefGoogle Scholar
  6. 6.
    Wain-Hobson S, Sonigo P, Danos O, Coles S, Alizon M (1985) Cell 40:9–17PubMedCrossRefGoogle Scholar
  7. 7.
    Ratner L, Haseltine W, Patarca R, Livak KJ, Starcich B, Josephs SF, Doran ER et al. (1985) Nature 313:277–284PubMedCrossRefGoogle Scholar
  8. 8.
    Muesing MA, Smith DH, Cabradilla CD, Benton CV, Lasky LA, Capon DJ (1985) Nature 313:450–458PubMedCrossRefGoogle Scholar
  9. 9.
    Sodroski J, Patarca R, Rosen CA, Wong-Staal F, Haseltine WA (1985) Science 229:74–77PubMedCrossRefGoogle Scholar
  10. 10.
    Allan JS, Coligan JE, Barin F, McLane MF, Sodroski JG, Rosen CA, Haseltine WA, Lee TH, Essex M (1985) Science 228:1091–1093PubMedCrossRefGoogle Scholar
  11. 11.
    Barin F, McLane MF, Allan JS, Lee TH, Groopman JE, Essex M (1985) Science 228:1094–1096PubMedCrossRefGoogle Scholar
  12. 12.
    Veronese FD, DeVico AL, Copeland TD, Oroszlan S, Gallo RC, Sarngadharan MG (1985) Science 229:1402–1405PubMedCrossRefGoogle Scholar
  13. 13.
    Weiss RA, Clapham PR, Cheingsong-Popov R, Dalgleish AG, Carne CA, Weller IVD, Tedder RS (1985) Nature 316:69–72PubMedCrossRefGoogle Scholar
  14. 14.
    Robert-Guroff M, Brown M, Gallo RC (1985) Nature 316:72–74PubMedCrossRefGoogle Scholar
  15. 15.
    Robey WG, Safai B, Oroszlan S, Arthur LO, Gonda MA, Gallo RC, Fischinger PJ (1985) Science 228:593–595PubMedCrossRefGoogle Scholar
  16. 16.
    Matthews TJ, Langlois AJ, Robey WG, Chang NT, Gallo RC, Fischinger PJ, Bolognesi DP (in press) Proc Natl Acad Sci USAGoogle Scholar
  17. 17.
    Ho DD, Rota TR, Hirsch MS (1985) N Engl J Med 312:649–650PubMedCrossRefGoogle Scholar
  18. 18.
    Wong-Staal F, Shaw GM, Hahn BH, Salahuddin SZ, Popovic M, Markham P, Redfield R, Gallo RC (1985) Science 229:759–762PubMedCrossRefGoogle Scholar
  19. 19.
    Starcich BR, Hahn BH, Shaw GM, McNeely PD, Modrow S, Wolf H, Parks ES et al. (in press) ScienceGoogle Scholar
  20. 20.
    Montagnier L, Clarel F, Krust B, Chamaret S, Rey F, Barre-Sinoussi F, Chermann JC (1985) Virology 144:283–289PubMedCrossRefGoogle Scholar
  21. 21.
    Schafer W, Bolognesi DP (1977) Contemp Top Immunobiol 6:127–167PubMedGoogle Scholar
  22. 22.
    Lee TH, Essex M, de Noronha F, Azocar J (1982) Cancer Res 42:3995–3999PubMedGoogle Scholar
  23. 23.
    Arthur L et al. (in press) J ImmunolGoogle Scholar
  24. 24.
    Popovic M, Sarngadharan MG, Read E, Gallo RC (1984) Science 224:497–500PubMedCrossRefGoogle Scholar
  25. 25.
    Poiesz BJ, Ruscetti FW, Gazdar AF, Bunn PA, Minna JD, Gallo RC (1980) Proc Natl Acad Sci USA 77:7415–7419PubMedCrossRefGoogle Scholar
  26. 26.
    Robey WG, Arthur LO, Matthews TJ, Langlois A, Copeland TD, Oroszlan S, Bolognesi DP, Gilden RV, Fischinger PJ (in press) Proc Natl Acad Sci USAGoogle Scholar
  27. 27.
    Chang TW, Kato I, McKinney S, Chanda P, Barone AD, Wong-Staal F, Gallo RC, Chang NT (1985) Biotechnology 3:905–909CrossRefGoogle Scholar
  28. 28.
    Porath J, Kristiansen T (1975) In: Neurath H, Hill RL (eds) The proteins, vol 1. Academic, New York, p 95Google Scholar
  29. 29.
    Essex M, Allan J, Kanki P, McLane MF, Malone G, Kitchen L, Lee TH (1985) Ann Intern Med 103:700–703PubMedGoogle Scholar
  30. 30.
    Fischinger PJ, Schafer W, Bolognesi DP (1976) Virology 71:169–184PubMedCrossRefGoogle Scholar

Copyright information

© Springer-Verlag Berlin Heidelberg 1987

Authors and Affiliations

  • Th. J. Matthews
    • 1
  • A. J. Langlois
    • 1
  • W. G. Robey
    • 2
  • N. T. Chang
    • 3
  • R. C. Gallo
    • 4
  • P. J. Fischinger
    • 2
  • D. P. Bolognesi
    • 1
  1. 1.Department of SurgeryDuke University Medical SchoolDurhamUSA
  2. 2.Office of DirectorNational Cancer Institute-Frederick Cancer Research Facility (NCI-FCRF)FrederickUSA
  3. 3.Center for BiotechnologyBaylor College of MedicineHoustonUSA
  4. 4.Laboratory of Tumor Cell BiologyNational Cancer Institute, National Institutes of HealthBethesdaUSA

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