Modern Trends in Human Leukemia VII pp 367-368 | Cite as
Use of Fluorescence-Activated Cell Sorting to Select Hybrid Hybridomas Producing Bispecific Monoclonal Antibodies
Conference paper
Abstract
Antibodies containing two different antigenbinding sites were prepared for the first time in 1961 by Nisonoff and Rivers [1] by dissociating two different antibodies and reassociating the mixture of half molecules. But this method of chemical recombination has some disadvantages because it results in protein denaturation and therefore loss of antibody activity.
Keywords
Ascitic Fluid Parental Cell Line Antibody Activity Bispecific Antibody Incubation Sequence
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References
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Copyright information
© Springer-Verlag Berlin Heidelberg 1987