NAD(P)H Oxidase in Non-Phagocytic Cells

  • A. Görlach
Conference paper
Part of the Yearbook of Intensive Care and Emergency Medicine book series (YEARBOOK, volume 1998)


The importance of reactive oxygen intermediates (ROI) is evident from the vast literature describing the involvement of free radicals in the pathogenesis of various disorders including neurological (Alzheimer’s disease, Parkinson’s disease), viral human immunodeficiency virus (HIV) and acquired immunodeficiency syndrome (AIDS), and degenerative (atherosclerosis, cancer, cataract) diseases.


NADPH Oxidase Reverse Transcriptase Polymerase Chain Reaction Chronic Granulomatous Disease Carotid Body Cytochrome B558 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.


  1. 1.
    Jones OTG (1994) The regulation of superoxide production by the NADPH oxidase of neutrophils and other mammalian cells. Bioessays 16: 919–922PubMedCrossRefGoogle Scholar
  2. 2.
    Khan AU, Wilson T (1995) Reactive oxygen species as cellular messengers. Chem Biol 2: 437–445PubMedCrossRefGoogle Scholar
  3. 3.
    Cross AR, Jones OTG (1991) Enzymatic mechanisms of superoxide production. Biochem Biophys Acta 1057: 281–298PubMedCrossRefGoogle Scholar
  4. 4.
    Leusen JHW, Verhoeven AJ, Roos D (1996) Interactions between the components of the human NADPH oxidase: Intrigues in the phox family. J Lab Clin Med 128: 461–476PubMedCrossRefGoogle Scholar
  5. 5.
    Curnutte JT (1993) Chronic granulomatous disease: the solving of a clinical riddle. Clin Immunol Immunopath 67: S2–S15CrossRefGoogle Scholar
  6. 6.
    DeLeo FR, Quinn M (1996) Assembly of the phagocyte NADPH oxidase: molecular interaction of oxidase proteins. J Leukoc Biol 60: 677–691PubMedGoogle Scholar
  7. 7.
    Saythyamoorthy M, deMendez I, Adams AG, Leto TL (1997) p40phox down-regulates NADPH oxidase activity through interactions with its SH3 domain. J Biol Chem 272: 9141–9146Google Scholar
  8. 8.
    Henderson LM, Thomas S, Banting G, Chappell JB (1997) The arachidonate-activable, NADPH oxidase-associated H+ channel is contained within the multi-membrane-spanning N-terminal region of gp91-phox. Biochem J 325: 701–705PubMedGoogle Scholar
  9. 9.
    Gorlach A, Lee PL, Roester J, et al (1997) A p47-phox Pseudogene carries the most common mutation causing p47-p/iox-deficient chronic granulomatous disease. J Clin Invest 100: 1907–1918PubMedCrossRefGoogle Scholar
  10. 10.
    Lien LL, Lee Y Orkin SH (1997) Regulation of the myeloid-cell-expressed human gp91phox gene as studied by transfer of yeast artificial chromosome clones into embryonic stem cells: suppression of a variegated cellular pattern of expression requires a full complement of distant cis elements. Mol Cell Biol 17: 2279–2290PubMedGoogle Scholar
  11. 11.
    Li SL, Valente AJ, Zhao SJ, Clark RA (1997) Pu.I is essential for p47(phox) promoter activity in myeloid cells. J Biol Chem 272: 17802–17809PubMedCrossRefGoogle Scholar
  12. 12.
    Lander HM (1997) An essential role for free radicals and derived species in signal transduction. FASEB J 11: 118–124PubMedGoogle Scholar
  13. 13.
    Irani K, Xia Y, Zweier JL, et al (1997) Mitogenic signaling mediated by oxidants in Ras transformed fibroblasts. Science 275: 1649–1652PubMedCrossRefGoogle Scholar
  14. 14.
    van Klaveren RJ, Roelant C, Boogaerts M, Demedts M, Nemery B (1997) Involvement of an NAD(P)H oxidase-like enzyme in superoxide anion and hydrogen peroxide generation by rat type II cells. Thorax 52: 465–471PubMedCrossRefGoogle Scholar
  15. 15.
    O’Donnell VB, Tew DG, Jones OTG, England PJ (1993) Studies on the inhibitory mechanism of iodonium compounds with specific reference to neutrophil NADPH oxidase. Biochem J 290: 41–49PubMedGoogle Scholar
  16. 16.
    Bunn HF, Poyton RO (1996) Oxygen sensing and molecular adaptation to hypoxia. Physiol Rev 76: 839–885PubMedGoogle Scholar
  17. 17.
    Acker H (1994) Mechanisms and meaning of cellular oxygen sensing in the organism. Resp Physiol 95: 1–10CrossRefGoogle Scholar
  18. 18.
    Gorlach A, Holtermann G, Jelkmann W, et al (1993) Photometric characteristics of haem proteins in erythropoietin-producing hepatoma cells (HepG2). Biochem J 290: 771–776PubMedGoogle Scholar
  19. 19.
    Fandrey J, Frede S, Jelkmann W (1994) Role of hydrogen peroxide in hypoxia-induced erythropoietin production. Biochem J 303: 507–510PubMedGoogle Scholar
  20. 20.
    Acker H, Bölling B, Delpiano MA, Dufau E, Görlach A, Holtermann G (1992) The meaning of H2O2 generation in carotid body cells for p02 chemoreception. J Auton Nerv Syst 41: 41–52PubMedCrossRefGoogle Scholar
  21. 21.
    Kummer W, Acker H (1995) Immunohistochemical detection of four subunits of neutrophil NAD(P)H oxidase in type I cells of carotid body. J Appl Physiol 78: 1904–1909PubMedGoogle Scholar
  22. 22.
    Wang D, Youngson C, Wong V, et al (1996) NADPH-oxidase and a hydrogen peroxide-sensitive K+ channel may function as an oxygen sensor complex in airway chemoreceptors and small lung cell carcinoma cell lines. Proc Natl Acad Sci USA 93: 13182–13187PubMedCrossRefGoogle Scholar
  23. 23.
    Youngson C, Nurse C, Yeger H, et al (1997) Immunocytochemical localization of 02-sensing proteins (NADPH oxidase) in chemoreceptor cells. Microsc Res Tech 37: 101–106PubMedCrossRefGoogle Scholar
  24. 24.
    Kummer W, Acker H (1997) Cytochrome b558 and hydrogen peroxide production in small intensely fluorescent cells of sympathetic ganglia. Histochem Cell Biol 197: 151–158CrossRefGoogle Scholar
  25. 25.
    Marshall C,Mamary AJ,Verhoeven AJ, Marshall BE (1996) Pulmonary artery NADPH-oxidase is activated in hypoxic pulmonary vasoconstriction. Am J Resp Cell Mol Biol 15: 633–644Google Scholar
  26. 26.
    Brandes RP, Barton M, Philippens KHM, Schweitzer G, Mügge A (1997) Endothelial-derived superoxide anions in pig coronary arteries: evidence from lucigene chemiluminescence and histochemical techniques. J Physiol 500: 331–342PubMedGoogle Scholar
  27. 27.
    Jones SA, O’Donnell V, Wood JD, Broughton JP, Hughes EJ, Jones OTG (1996) Expression of phagocyte NADPH oxidase components in human endothelial cells. Am J Physiol 271: H1626–H1634PubMedGoogle Scholar
  28. 28.
    Zulueta JJ,Yu FS,Hertig I A, Thannickal VJ,Hassoun PM (1995) Release of hydrogen peroxide in response to hypoxia-reoxygenation: role of an NAD(P)H oxidase-like enzyme in endothelial cell plasma membrane. Am J Resp Cell Mol Biol 12: 41–49CrossRefGoogle Scholar
  29. 29.
    Kinnula VK, Mirza Z, Cerapo JD, Whorton AR (1993) Modulation of hydrogen peroxide release from vascular endothelial cells by oxygen. Am J Resp Cell Mol Biol 9: 603–609Google Scholar
  30. 30.
    Bhunia AK, Han H, Snowden A, Chatterjee S (1997) Redox-regulated signaling by lactosylceramide in the proliferation of human aortic smooth muscle cells. J Biol Chem 272: 15642–15649PubMedCrossRefGoogle Scholar
  31. 31.
    Griendling KK, Minieri CA, Ollerenshaw JD, Alexander RW (1994) Angiotensin II stimulates NADH and NADPH oxidase activity in cultured vascular smooth muscle cells. Circ Res 74: 1141–1148PubMedGoogle Scholar
  32. 32.
    Rajagopalan S, Kurz S, Münzel T, et al (1996) Angiotensin II-mediated hypertension in the rat increases vascular superoxide production via membrane NADH/NADPH oxidase activation. J Clin Invest 97: 1916–1923PubMedCrossRefGoogle Scholar
  33. 33.
    Ushio-Fukai M, Mazia-Zafari A, Fukui T, Ishizaka N, Griendling KK (1996) p22phox is a critical component of the superoxide-generating NADH/NADPH oxidase system and regulates angiotensin II-induced hypertrophy in vascular smooth muscle cells. J Biol Chem 271: 23317–23321Google Scholar
  34. 34.
    Jones SA, Hancock JT, Jones OTG, Neubauer A, Topley N (1995) The expression of NADPH oxidase components in human glomerular mesangial cells: detection of protein and mRNA for p47phox, p67phox, and p22phox. J Am Soc Nephrol 5: 1483–1491PubMedGoogle Scholar
  35. 35.
    Neale TJ, Ullrich R, Ojha P, Poczewski H, Verhoeven AJ, Kerjaschki D (1993) Reactive oxygen species and neutrophil respiratory burst cytochrome b558 are produced by kidney glomerular cells in passive Heymann nephritis. Proc Natl Acad Sei USA 90: 3645–3649CrossRefGoogle Scholar
  36. 36.
    Cui XL, Douglas JG (1997) Arachidonic acid activates c-jun N-terminal kinase through NADPH oxidase in rabbit proximal tubular epithelial cells. Proc Natl Acad Sei USA 94: 3771–3776CrossRefGoogle Scholar
  37. 37.
    Meier B, Jesaitis AJ, Emmendoerfer A, Roesler J, Quinn MT (1993) The cytochrome b-558 molecules involved in the fibrobalst and polymorphonuclear leucocyte superoxide-generating NADPH oxidase systems are structurally and genetically distinct. Biochem J 289: 481–486PubMedGoogle Scholar
  38. 38.
    Jones SA, Wood JD, Coffey MJ, Jones OTG (1994) The functional expression of p47-phox and p67-phox may contribute to the generation of superoxide by an NADPH oxidase-like system in human fibroblasts. FEBS Lett 355: 178–182PubMedCrossRefGoogle Scholar
  39. 39.
    Hiran TS, Moulton PJ, Hancock JT (1997) Detection of superoxide and NADPH oxidase in porcine articular chondrocytes. Free Rad Biol Med 23: 736–743PubMedCrossRefGoogle Scholar
  40. 40.
    Rathakrishanan C, Tiku K, Raghavan A, Tiku MT (1992) Release of oxygen radicals by articular chondrocytes: a study of luminol-dependent chemiluminescence and hydrogen peroxide secretion. J Bone Min Res 7: 1139–1148CrossRefGoogle Scholar
  41. 41.
    Steinbeck MJ, Appel WH, Verhoeven AJ, Karnovsky MJ (1994) NADPH-oxidase expression and in situ production of superoxide by osteoclasts actively resorbing bone. J Cell Biol 126: 765–772PubMedCrossRefGoogle Scholar

Copyright information

© Springer-Verlag Berlin Heidelberg 1998

Authors and Affiliations

  • A. Görlach

There are no affiliations available

Personalised recommendations