A Growth-Factor Dependent B-Cell Hybridoma

  • P. M. Lansdorp
  • L. A. Aarden
  • J. Calafat
  • W. P. Zeiljemaker
Part of the Current Topics in Microbiology and Immunology book series (CT MICROBIOLOGY, volume 132)

Abstract

Injection of pristane (2,6,10,14 tetramethylpentadecane) into the peritoneal cavity of Balb/c mice has been used to induce plasmacytomas (1) and to induce a microenvironment that will support the growth of transplanted plasmacytomas derived from primary hosts (2) and B-cell hybridomas (3). In vitro growth of primary plasmacytomas (4) and B-cell hybrids immediately after fusion (5) requires the addition of “feeder” cells to the culture medium. In previous studies we have shown that this feeder-cell requirement of B-cell hybrids can be overcome by the addition of the supernatant of human endothelial cell cultures to the culture medium (6,7). The characterization of this growth-promoting activity has been hampered by the lack of rapid and reproducible assay.

Keywords

Conditioned Medium Rough Endoplasmatic Reticulum Human Peripheral Blood Leukocyte Reproducible Assay Fetal Skin Fibroblast 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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Copyright information

© Springer-Verlag Berlin · Heidelberg 1986

Authors and Affiliations

  • P. M. Lansdorp
    • 1
  • L. A. Aarden
    • 1
  • J. Calafat
    • 2
  • W. P. Zeiljemaker
    • 1
  1. 1.Central Laboratory of the Netherlands Red Cross Blood Transfusion Service and Laboratory for Experimental and Clinical ImmunologyUniversity of AmsterdamAmsterdamThe Netherlands
  2. 2.Netherlands Cancer InstituteAmsterdamThe Netherlands

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