Methods for the Experimental Evaluation of Neuromuscular Blocking Agents

  • D. A. Kharkevich
Chapter
Part of the Handbook of Experimental Pharmacology book series (HEP, volume 79)

Abstract

In the quest for more perfect neuromuscular blocking agents numerous techniques have been suggested (see reviews by LEWIS and MUIR 1959 a, b, c; BOWMAN 1964; BARLOW 1968; KHARKEVICH 1969; CHEYMOL and BOURILLET 1972; MACLAGAN 1976). They are mainly based on the ability of the agents to block the neuromuscular transmission on the electrical stimulation of the corresponding motor nerve and to cause myorelaxation. Nondepolarizing agents can also be tested by their antagonism with cholinomimetics (by the elimination of the stimulating effect of the latter). At the same time, depolarizing neuromuscular blocking agents can stimulate the skeletal muscles, thus leading to more or less stable contractions (from twitch to contracture). Neuromuscular blocking agents are studied in various animal species and on their neuromuscular preparations both in vivo, and in vitro. The experiments are carried out in vivo on conscious animals and on animals immobilized by general anesthetics or decerebrated animals. In such experiments the agents tested are usually administered intravenously or intraperitoneally (rarely subcutaneously). In addition, close-arterial injection is sometimes used.

Keywords

Gastrocnemius Muscle Neuromuscular Blocking Neuromuscular Transmission Artificial Ventilation Neuromuscular Blocking Agent 
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  • D. A. Kharkevich

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