Isolation, Properties, and Functions of Tonoplast ATPase from Higher Plants

  • Willy Lin
  • George J. Wagner
Conference paper

Abstract

It has long been speculated that tonoplast ATPase plays important roles in regulating solute concentration in plant cells and solute transport at the tonoplast (Dainty, 1968 ; Hodges, 1976 ; Leigh, 1983 ; Mac Robbie, 1979 ; Marin, Crétin, d’Auzac, 1982 ; Wagner, 1983) but until recently detailed biochemical investigations were limited by the lack of methods for isolating and identifying membrane preparation from plant tissues. In the mid-1970s and early-1980s methods were developed which allowed the isolation of large number of higher plant vacuoles thus for tonoplast membrane vesicles relatively free of contamination by other organelles (Leigh, Branton, 1976 ; Lorz, Harms, Potrykus, 1976 ; Wagner, Siegelman, 1976). Also, methods were developed for isolating tightly sealed membrane vesicles from tissue homogenates which some have suggested to be tonoplast derived (Bennett, O’Neill, Spanswick, 1984 ; Dupont, Bennett, Spanswick, 1982 ; Dupont, Giorgi, Spanswick, 1982 ; Poole, Briskin, Kratky, Johnstone, 1984 ; Sze, 1980). Using these methods a number of workers have accumulated evidence which indicate that tonoplast does possess a membrane-bound ATPase activity and associated proton pump.

Keywords

ATPase Activity Membrane Vesicle Proton Transport Crassulacean Acid Metabolism Plant Tonoplast Vesicle 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.
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Copyright information

© Springer-Verlag Berlin Heidelberg 1985

Authors and Affiliations

  • Willy Lin
    • 1
  • George J. Wagner
    • 2
  1. 1.Central Research and Development DepartmentExperimental Station E.I. Du Pont de Nemours and CompanyWilmingtonUSA
  2. 2.Department of Agronomy, College of AgricultureUniversity of KentuckyLexingtonUSA

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