Evaluation of an Immunodiagnostic Test Based on the Use of Wuchereria bancrofti Mf ES Antigen in ELISA for Bancroftian Filariasis

  • B. C. Harinath
  • A. Malhotra
Conference paper
Part of the Health Systems Research book series (HEALTH)

Abstract

Bancroftian filariasis caused by human filarial parasite Wuchereria bancrofti affecting 250 million people in the world is a major public health problem in developing tropical countries. Parasitological examination techniques are not useful for diagnosis of filariasis with low microfilaraemia, occult or chronic infections. Studies using homologous antigen for diagnosis are scanty. Presence of filarial antibody in filariasis was detected by indirect haemagglutination, indirect fluorescent antibody test and penicillinase enzyme linked immunosorbent assay (Kaliraj et al. 1981). Fractionation of soluble microfilarial antigen by Sephadex G-150 gel filtration followed by DEAE-cellulose chromatography gave mfS3e antigen fraction which was useful in distinguishing microfilaraemia from endemic normals and clinical filariasis by ELISA (Kaliraj et al. 1982). Wuchereria bancrofti excretory and secretory (ES) antigens were found to be highly sensitive and as little as 0.35 ng of antigen protein per well in ELISA was found to be sufficient in detection of antibody in filarial sera (Kharat et al. 1982). Filter paper blood sample has been used for detection of filarial antibody by ELISA (Malhotra et al. 1982).

Keywords

Indirect Fluorescent Antibody Test Bancroftian Filariasis Homologous Antigen Endemic Normal Indirect Hemagglutination Test 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.
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References

  1. Kaliraj P, Ghirnikar SN, Harinath, BC (1981) Immunodiagnosis of bancroftian filariasis: comparative efficiency of the indirect hemagglutination test, indirect fluorescent antibody test, and enzyme-linked immunosorbent assay done with Wuchereria bancrofti microfilarial antigens. Am J Trop Med Hyg 30 (5): 982–987.PubMedGoogle Scholar
  2. Kaliraj P, Harinath BC, Ghirnikar SN(1982) Fractionation and evaluation of Wuchereria bancrofti microfilarial antigens in immunodiagnosis of bancroftian filariasis. Ind J Exit Biol 20: 440–444.Google Scholar
  3. Kharat I, Harinath BC, Ghirnikar SN (1982) Antibody analysis in human filariasis sera by ELISA using Wuchereria bancrofti microfilariae culture antigen. Ind J Expt Biol 20: 378–380.Google Scholar
  4. Malhotra A, Reddy MVR, Naidu JN, Ghirnikar SN, Harinath BC (1982) Detection of filarial infection using Wuchereria bancrofti microfilariae culture antigen and filter paper blood samples in enzyme linked immunosorbent assay. J Biosci 4: 507–512.CrossRefGoogle Scholar

Copyright information

© Springer Verlag , Berlin Heidelberg 1984

Authors and Affiliations

  • B. C. Harinath
    • 1
  • A. Malhotra
    • 1
  1. 1.Department of BiochemistryMahatma Gandhi Institute of Medical SciencesSevagram, WardhaIndia

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