Uses and Abuses of Diagnostic Electron Microscopy
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It is almost exactly 20 years since it was realised that the electron microscopy technique of negative staining (Brenner and Home 1959) could be applied in a practical fashion to the recognition of viruses not only in purified suspension, but also in association with cell material (Almeida and Howatson 1963). At first the technique was applied to the visualisation of viruses and the determination of their substructure. Very shortly afterwards it was realised that the technique could be applied to clinical material either directly or after one cycle of growth, either in egg or in tissue culture. At this time smallpox was still extant, and by far the most important use of the method was the differentation between variola and varicella virus (Peters et al. 1962; Nagington 1964).
KeywordsSickle Cell Anaemia Virus Particle Negative Staining Rubella Virus Yellow Fever Virus
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