A Microculture Method for Production of Monoclonal Antibodies

  • P. I. Terasaki
  • P. Kaszubowski
Conference paper

Abstract

The technique of producing monoclonal antibodies has become somewhat standardized [1]. Although devices for transferring supernatants and adding fluids have been commercially developed, the technique remains a tedious one. Repetitive works is required to search out the clone that is producing the desired antibody. Since the laws of chance determine succes in finding certain rare antibodies, various strategies must be developed. Among these are modifications of the antigen so that it is presented in different ways, different means of immunizing the mice, methods of concentrating the responding cells, and so on. We wish to discuss here one strategy that we consider to be basic.

Keywords

Repetitive Work Monoclonal Antibody OKT5 Antibody OKT5 Thymic Factor Culture Tray 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.
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References

  1. 1.
    Fazekas de St. Groth S, Scheidegger D (1980) Production of monoclonal antibodies: Strategy and tactics. J Immunol Methods 35: 1–21CrossRefGoogle Scholar
  2. 2.
    Terasaki PI, Bernoco D, Park MS, Ozturk G, Iwaki Y (1978) Microdroplet testing for HLA-A, -B, -C, and -D antigens. Am J Clin Pathol 69: 103–120PubMedGoogle Scholar
  3. 3.
    Landsdorp PM, Oosterhof F, Astaldi GCB, Zeijlemaker WP (1982) Detection of HLA antigens on blood platelets and lymphocytes by means of monoclonal antibodies in an ELISA technique. Tissue Antigens 19: 11–19CrossRefGoogle Scholar

Copyright information

© Springer-Verlag Berlin Heidelberg 1984

Authors and Affiliations

  • P. I. Terasaki
  • P. Kaszubowski

There are no affiliations available

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