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Coronavirus Infection in Rats

  • K. Nagashima
  • H. Wege
  • R. Meyermann
  • V. ter Meulen
Conference paper
  • 40 Downloads

Summary

Murine coronavirus JHM infection in rats can be accompanied by different types of demyelinating central nervous system (CNS) diseases. Infection of suckling rats results in an acute, disseminated encephalomyelitis affecting both neurons and oligodendroglia cells. After an average incubation period of 2–3 weeks, weanling rats develop a predominantly subacute demyelinating encephalomyelitis (SDE) characterized by perivascular cuffings, demyelinated foci, and destruction of oligodendroglia cells, leaving the neurons and axons intact. After 3–8 months, some of the weanling rats which do not after 3 weeks display any CNS affection develop a neurological disease with neuropathological changes similar to SDE. However, in addition to demyelination, remyelination is detectable in this disease pattern. Infectious coronavirus can be isolated from all diseased animals. The infection of weanling rats with coronavirus JHM offers the possibility to explore experimentally the pathogenetic mechanisms responsible for a virus-associated demyelinating process.

Keywords

Myelin Sheath Demyelinating Disease Mouse Hepatitis Virus Coronavirus Infection Spongy State 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

References

  1. 1.
    Bailey OT, Pappenheimer AM, Cheever FS, Daniels JB (1949) A murine virus (JHM) causing disseminated encephalomyelitis with extensive destruction of myelin. II. Pathology. J Exp Med 90:195–212PubMedCrossRefGoogle Scholar
  2. 2.
    Blakemore WF (1974) Pattern of remyelination in the CNS. Nature 249:575–578CrossRefGoogle Scholar
  3. 3.
    Blakemore WF (1975) Remyelination by Schwann cells of axons demyelinated by intraspinal injection of 6-aminonicotinamide in the rat. J Neurocytol 4:745–757PubMedCrossRefGoogle Scholar
  4. 4.
    Haspel VM, Lampert PW, Oldstone MBA (1978) Temperature-sensitive mutants of mouse hepatitis virus produce a high incidence of demyelination. Proc Natl Acad Sci USA 75:4033–4036PubMedCrossRefGoogle Scholar
  5. 5.
    Lampert PW, Sims JK, Kniazeff AJ (1973) Mechanism of demyelination in JHM virus encephalomyelitis. Electron microscopic studies. Acta Neuropathol. (Berl.) 24, 76–85Google Scholar
  6. 6.
    Lucas A, Coulter M, Anderson R, Dales S, Flintoff W (1978) In vivo and in vitro models of demyelinating diseases II. Persistence and host-regulated thermosensitivity in cells of neuronal derivation infected with mouse hepatitis and measles viruses. Virology 88:325–337PubMedCrossRefGoogle Scholar
  7. 7.
    Nagashima K, Wege H, ter Meulen V (1978) Early and late CNS-effects of Coronavirus infection in rats. Adv Exp Med Biol 100:395–409PubMedGoogle Scholar
  8. 8.
    Nagashima K, Wege H, Meyermann R, ter Meulen V (1978) Coronavirus induced subacute demyelinating encephalomyelitis in rats. A morphological analysis. Acta Neuropathol (Berl) 44:63–70CrossRefGoogle Scholar
  9. 9.
    Nagashima K, Wege H, Meyermann R, ter Meulen V (1979) Demyelinating Encephalomyelitis induced by a long-term Coronavirus infection in rats. Acta Neuropathol (Berl) 45:205–213CrossRefGoogle Scholar
  10. 10.
    Stohlman SA, Weiner LP (1978) Stability of neurotropic mouse hepatitis virus (JHM strain) during chronic infection of neuroblastoma cell. Arch Virol 57:53–61PubMedCrossRefGoogle Scholar
  11. 11.
    Stohlman SA, Frelinger JA (1978) Resistance to fatal central nervous system disease by mouse hepatitis virus, strain JHM. I. Genetic analysis. Immunogenetics 6:277–282CrossRefGoogle Scholar
  12. 12.
    ter Meulen V, Wege H (1978) Virus infection in demyelinating diseases. Adv Exp Med Biol 100:383–394PubMedGoogle Scholar
  13. 13.
    Virelizier JL, Dayan AD, Allison AC (1975) Neuropathological effects of persistent infection of mice by mouse hepatitis virus. Infect Immun 12:1127–1140PubMedGoogle Scholar
  14. 14.
    Wege H, Müller A, ter Meulen V (1978) Genomic RNA of the murine Coronavirus JHM. J Gen Virol 41:217–227PubMedCrossRefGoogle Scholar
  15. 15.
    Wege H, Wege H, Nagashima K, ter Meulen V (1979) Structural Polypeptides of the murine Coronavirus JHM. J Gen Virol 42:37–47PubMedCrossRefGoogle Scholar
  16. 16.
    Weiner, LP (1973) Pathogenesis of demyelination induced by a mouse hepatitis virus (JHM virus). Arch Neurol 28:293–303Google Scholar

Copyright information

© Springer-Verlag Berlin Heidelberg 1980

Authors and Affiliations

  • K. Nagashima
    • 1
  • H. Wege
    • 2
  • R. Meyermann
    • 3
  • V. ter Meulen
    • 2
  1. 1.Department of Pathology, Faculty of MedicineUniversity of TokyoBunkyo-ku, Tokyo 113Japan
  2. 2.Institut für Virologie und ImmunobiologieUniversität WürzburgWürzburgGermany
  3. 3.Neuropathologisches InstitutUniversität GöttingenGöttingenGermany

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