Defective Viral Infection: The Rescue of Virus from Antigen-Negative Cells
There is epidemiologic evidence for a viral infection in multiple sclerosis (MS). Over the years, frequent attempts to rescue virus and demonstrate viral antigens in the brain of MS patients have failed. There have been a number of isolates, but none have been found to play a role in the etiology of the disease. We have been studying an animal model of viral-induced acute and chronic demyelination. The virus, a mouse coronavirus, JHM, is a neurotropic strain of mouse hepatitis virus. In order to better understand JHM virus chronic infection, we have developed an in vitro carrier culture system of persistently infected mouse neuroblastoma cells .
KeywordsMultiple Sclerosis Multiple Sclerosis Patient Neuroblastoma Cell Viral Antigen Infectious Virus
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Knight P, Duff R, Glaser R, Rapp F (1973) Characteristics of release of measles virus from latently infected cells after co-cultivation with B5C-1 cells. Intervirology 2:287–298CrossRefGoogle Scholar
Lai MMC, Stohlman SA (1978) RNA of mouse hepatitis virus. J Virol 26:236–242PubMedGoogle Scholar
Neff JM, Enders JF (1968) Poliovirus replication and cytopathogenicity in monolayer hamster cell cultures fused with beta propiolactone-inactivated Sendai virus. Proc Soc Exp Biol Med 127:266–267Google Scholar
Pontecorvo G (1975) Production of mammalian somatic cell hybrids by means of polyethylene glycol treatment. Somatic Cell Genet 1:397–400PubMedCrossRefGoogle Scholar
Prasad K (1975) Differentiation of neuroblastoma cells in culture. Biol Rev 50:129–165PubMedCrossRefGoogle Scholar
Stohlman SA, Weiner LP (1978) Stability of neurotropic mouse hepatitis virus (JHM strain) during chronic infection of neuroblastoma cells. Arch Virol 57:53–61PubMedCrossRefGoogle Scholar
Stohlman SA, Wisseman CL, Eylar, OR, Silverman DJ (1975) Dengue virus-induced modifications of host cell membranes. J Virol 16:1017–1026PubMedGoogle Scholar
Weiner LP, Herndon RM, Naragan O, Johnson RT (1972) Further studies of an S\40-like virus isolated from human brain. J Virol 10:147–152PubMedGoogle Scholar
Worblewska Z, Santoli D, Gilden D, Lewandowski L, Koprowski H (1976) Persistent parainfluenza type 1 (6/94) infection of brain cells in tissue culture. Arch Virol 50:287–303CrossRefGoogle Scholar
© Springer-Verlag Berlin Heidelberg 1980