Fundamental Aspects of Neoplasia pp 269-277 | Cite as
An Inhibitor of DNA Polymerase Produced by Tumor Cells
Abstract
We have previously described the isolation and partial characterization of several DNA polymerases from the murine myeloma, MOPC 21 (1,2). In particular, we have called attention to a distinct enzyme from this line of cells (R-1 polymerase), which has the ability to utilize the synthetic polynucleotide duplex poly rA:oligo(dT)12–18 very efficiently. In this regard, R-1 polymerase resembles reverse transcriptase from the Rauscher murine leukemia virus, but it can be readily distinguished from the latter by its sedimentation coefficient and isolectric point. A summary of the comparative properties of the R-1 polymerase and the reverse transcriptase from the Rauscher murine leukemia virus are listed in Table 20-1.
Keywords
Multiple Myeloma Ammonium Sulfate Murine Leukemia Virus Sedimentation Coefficient Syngeneic MousePreview
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