The Role of the Escherichia Coli Galactose-Binding Protein in Galactose Transport and Chemotaxis

  • T. J. Silhavy
  • W. Boos
  • H. M. Kalckar
Conference paper
Part of the Colloquium der Gesellschaft für Biologische Chemie 25.–27. April 1974 in Mosbach/Baden book series (MOSBACH, volume 25)

Abstract

The galactose-binding protein (GBP) was first isolated by Anraku, and he suggested that GBP was involved in galactose transport in Escherichia coli (Anraku, 1968). The study of galactose transport in this organism is complicated by the existence of at least six transport systems with specificity for galactose (Kalckar, 1971; Rotman et al., 1968). This overlap made the design of selection procedures needed to obtain well defined mutants rather complex. Since no well characterized mutants were available, and since no other galactose derivatives were checked for binding, it was not possible at this time to tell which transport system required GBP.

Keywords

Difference Spectrum Tryptophan Residue Deuterium Oxide Shocked Cell Shock Fluid 
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Copyright information

© Springer-Verlag Berlin · Heidelberg 1974

Authors and Affiliations

  • T. J. Silhavy
    • 1
  • W. Boos
    • 1
  • H. M. Kalckar
    • 1
  1. 1.Department of Biological Chemistry, Harvard Medical School and the Biochemical Research LaboratoryMassachusetts General HospitalBostonUSA

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