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Femtosecond spectroscopy and vibrational coherence of membrane-bound RCs of Rhodobacter sphaeroides genetically modified at Positions M210 and L181

  • Marten H. Vos
  • Michael R. Jones
  • C. Neil Hunter
  • Jacques Breton
  • Jean-Christophe Lambry
  • Jean-Louis Martin
Conference paper

Abstract

The mechanism of primary charge separation in bacterial reaction centers is still subject to debate [1]. Amongst other questions, the functional role of coherent motions from non-thermalized vibrational states has to be evaluated. The potential energy surface of the excited donor state P* is strongly displaced with respect to the ground state for a number of low-frequency vibrational modes. This has been extensively documented by photochemical holeburning [2, 3, 4] and resonance Raman [5,6] studies. Using femtosecond spectroscopy to monitor oscillations in the stimulated emission band shape, we have previously shown that phase correlations for those modes are maintained on the timescale of electron transfer [7,8]. In the usual assumption that vibrational dephasing precedes or at most is concomitant with the processes of energy exchange, these observations imply that the entire vibrational manifold of P* is not thermalized on the timescale of ET.

Keywords

Potential Energy Surface Transient Absorption Spectrum Ground State Absorption Primary Charge Separation Bacterial Reaction Center 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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Copyright information

© Springer-Verlag Berlin Heidelberg 1996

Authors and Affiliations

  • Marten H. Vos
    • 1
  • Michael R. Jones
    • 2
  • C. Neil Hunter
    • 2
  • Jacques Breton
    • 3
  • Jean-Christophe Lambry
    • 1
  • Jean-Louis Martin
    • 1
  1. 1.Laboratoire d’Optique Appliquée, INSERM U275, CNRS URA1406Ecole Polytechnique-ENSTAPalaiseauFrance
  2. 2.Krebs Institute for Biomolecular Research and Robert Hill Institute for Photosynthesis, Department of Molecular Biology and BiotechnologyUniversity of Sheffield, Western BankSheffieldUK
  3. 3.CEN de SaclaySBE/DBCMGif-sur-Yvette CedexFrance

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