Change in Membrane Fluidity after pinocytosis of a new Cytokine IK on Resting and Activated T cells

  • Raffaele Pereno
  • Patricia Krief
  • Zohar Mishal
Conference paper
Part of the NATO ASI Series book series (volume 95)

Abstract

Pinocytosis is a mechanism by which the majority of eukaiyotic cells internalize external fluid. However, endocytosis has come to be used synonymously with pinocytosis. When considering pinocytosis, it is important to distinguish between two types of uptake. One involves specific sites on the cell surface, as moiphologically or biochemically distinguished, where surface binding is important and the uptake may depend on special mechanism. The other involves a non selective uptake of solute molecules that take place by suiface vesiculation and engulfment of medium and does not seem to use specific binding sites (Van Deurs et al., 1990).

Keywords

Major Histocompatibility Complex Class Membrane Fluidity Fluorescence Polarization Fluorescence Laser Scanning Confocal Microscopy Bare Lymphocyte Syndrome 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.
This is a preview of subscription content, log in to check access.

Preview

Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.

References

  1. Anel A., Naval J., Gonzales B., Torres JM., Mishal Z., Uriel J. and Pineiro A. (1990) Fatty acid metabolism in human lymphocytes. I.Time-course changes in fatty acid composition and membrane fluidity during blastic transformation of periferal blood lymphocytes. Biochim. Biophys. Acta 1044 pp 323–331PubMedGoogle Scholar
  2. Benoist, C. & Mathis, D. (1990) Regulation of Major Histocompatibility complex class II genes: X, Y and other letters of alphabet. Annu. Rev. Immunol., 8, 681–715.PubMedCrossRefGoogle Scholar
  3. Gorvel JP., Mawas C., Maroux S. and Mishal Z. (1984) Flow cytometry is a new method for the charaterization of intestinal plasma membrane. Biochem. J., 221, pp 453–457.PubMedGoogle Scholar
  4. Grant, J. and Scrutton M. (1978) Amines inhibit the clustering of (α2-macroglobulin and EGF on the fibroblast cell surface. Nature Vol.277 22 February 661–663Google Scholar
  5. Janeway C. A., Bottomly JR., Babic K., Conrad J., Conzen P.J., Jones S., Kaye B., Katz J., McVay M., Murphy L., D.B. and Tite J.P. (1984) Quantitative variation in la-antigen expression plays a central role in immunoregulation. Immunol. Today, 5, 99–105.CrossRefGoogle Scholar
  6. Krief, P. Augery-Bourget Y., Plaisance S., Merck M., Assier E., Tanchou V., Billard M., Boucheix C., Jasmin C. & Azzarone B. (1994) A new cytokine (IK) down-regulating HLA class II: Monoclonal antibodies, cloning and chromosome localization. Oncogene, 9, 3449–3456.PubMedGoogle Scholar
  7. Lechler R.I., Norcross M.A., and Germain R.N. (1985) Qualitative and quantitative studies of antigens-presenting cell function by using I-A-expressing L cells. The Journal of Immunology. Vol 135 pp 2914–2922.PubMedGoogle Scholar
  8. Matis L.A., Glimcher L.H., Paul W.E. and Schwartz R.H. (1983) Magnitude of response of Histocompatibility-restricted T-cell clones is a function of the product of the concentrations of antigen la molecules. Proc.Natl.Acad.Sci. USA Vol 80, pp 6019–6023.PubMedCrossRefGoogle Scholar
  9. Mazia D., Schatten G., and Sale W. (1975) Adhesion of cells to surfaces coated with polylysine. Journal of Cell Biology Vol 66, 198–200PubMedCrossRefGoogle Scholar
  10. Rodes JF., Bonnenfant J., Tremolieres A., and Brown S. (1995) Modulation of membrane fluidity and lipidic metabolism in transformed rat fibroblasts induced by the sesquiterpenic hormone farnesylacetone. Cytometry 19: 217–225.PubMedCrossRefGoogle Scholar
  11. Shinitzky, M. (1984) The lipid fluidity of cell membranes. CRC Press Inc. (ed) Physiology of Membrane Fluidity Vol I, Boca Raton Florida, pp 1–57Google Scholar
  12. Shinitzky, M. and Y. Barenholz, (1978) Fluidity parameters of lipid regions determined by fluorescence polarization.Biochim. Biophys. Acta 515, 367–396.PubMedGoogle Scholar
  13. Sillacci, P., A. Mottet, Steimle V., W. Reith, and B. Mach (1994) Developmental extinction of Major Histocompatibility Complex class II gene expression in plasmocytes is mediated by silencing of the transactivator gene CIITA. J. Exp. Med., Vol.180 1329–1336.CrossRefGoogle Scholar
  14. Van Deurs, B., O.W. Petersen, S. Olsnes, K. Sandvig, (1990) The ways of endocytosis. Int. Rev. Cytol., Vol. 117 131–177.Google Scholar

Copyright information

© Springer-Verlag Berlin Heidelberg 1996

Authors and Affiliations

  • Raffaele Pereno
    • 1
  • Patricia Krief
    • 1
  • Zohar Mishal
    • 2
  1. 1.INSERUM U268 Hôpital Paul BrousseVillejuifFrance
  2. 2.Laboratoire de CytométrieC.N.R.S UPS 47VillejuifFrance

Personalised recommendations