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Studies on the ATP-binding Site of Actin Using Site-directed Mutagenesis

  • Herwig Schüler
  • Elena Korenbaum
  • Uno Lindberg
  • Roger Karlsson
Part of the NATO ASI Series book series (volume 102)

Abstract

Hydrolysis of the actin-bound AT? is linked to actin filament turnover. However, neither the role nor the mechanism of the actin ATPase are well established. A novel role for the actin ATPase in muscle contraction has been proposed, making actin the principal force generator (1). The crystal structures of α- (2,3) and β-actin (4) suggest involvement of serine 14 (S14) and aspartic acid 157 (D157) in ATP hydrolysis (2,5). Myslik (5) suggested that the serine hydroxyl, polarized by the aspartic carboxyl, might be transiently phosphorylated under the hydrolysis reaction. According to his analysis (5) of the β-actin structure (4), the geometry of the ATP-site would favor an in-line attack on the ATPγP by the serine hydroxyl. This model was also inspired by the geometric homology of actin to the ATPase domain of Hsc70, which places alcoholic and acidic side chains at similar positions (6).

Keywords

ATPase Activity Actin Filament Motility Assay Myosin Binding Heat Shock Cognate Protein 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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Copyright information

© Springer-Verlag Berlin Heidelberg 1997

Authors and Affiliations

  • Herwig Schüler
    • 1
  • Elena Korenbaum
    • 1
  • Uno Lindberg
    • 1
  • Roger Karlsson
    • 1
  1. 1.Department of Cell Biology, The Wenner-Gren InstituteStockholm UniversityStockholmSweden

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