Mouse Hepatitis Virus Infection, Liver, Mouse

  • Stephen W. Barthold
Part of the Monographs on Pathology of Laboratory Animals book series (LABORATORY)


Gross lesions can occur in liver, intestine, and lymphoreticular organs. Intestinal lesions are described in detail elsewhere. Affected livers have random, small, pale or hemorrhagic foci to multiple confluent foci with depression of the capsular surface. The liver may be diffusely pale and covered with fibrinous peritoneal exudate. Infant mice can be runted, jaundiced, or may manifest neurologic signs, including tremor, incoordination, or convulsions (Piazza 1969). During the acute phase of infection, involution of lymph nodes, spleen, and thymus can occur. Recovered mice develop mild splenomegaly or lymphadenomegaly, particularly in cervical nodes. Athymic nude mice can become progressively cachectic (wasting disease). Their livers are contracted with rough, nodular surfaces (Ward et al. 1977), and splenomegaly can be pronounced (Ishida et al. 1978).


Hepatoencephalitis virus murine hepatitis virus infection mouse Coronavirus infection 


  1. Bang FB (1978) Genetics of resistance of animals to viruses. I. Introduction and studies in mice. Adv Virus Res 23:269–348PubMedCrossRefGoogle Scholar
  2. Barthold S (1985) Research complications and state of knowledge of rodent coronaviruses. In: Hamm TE Jr (ed) Complications of viral and mycoplasmal infections in rodents to toxicology research and testing. Hemisphere, Washington DC, pp 53–89Google Scholar
  3. Barthold SW (1986a) Mouse hepatitis virus biology and epizootiology. In: Bhatt PN, Jacoby RO, Morse HC II, New AE (eds) Viral and mycoplasmal infections of laboratory rodents: effects on biomedical research. Academic, Orlando, pp 571–601Google Scholar
  4. Barthold SW (1986b) Olfactory neural pathway in mouse hepatitis virus nasoencephalitis. Acta Neuropathol (Berl) 76:502–506CrossRefGoogle Scholar
  5. Barthold SW, Smith AL (1983) Mouse hepatitis virus S in weanling Swiss mice following intranasal inoculation. Lab Anim Sci 33:355–360PubMedGoogle Scholar
  6. Barthold SW, Smith AL (1984) Mouse hepatitis virus strain-related pattern of tissue tropism in suckling mice. Arch Virol 81:103–112PubMedCrossRefGoogle Scholar
  7. Barthold SW, Smith AL (1987) Response of genetically susceptible and resistant mice to mice to intranasal inoculation with mouse hepatitis virus JHM. Virus Res 7:225–239PubMedCrossRefGoogle Scholar
  8. Barthold SW, Smith AL (1989a) Duration of challenge immunity to coronavirus JHM in mice. Arch Virol 107:171–177PubMedCrossRefGoogle Scholar
  9. Bartold SW, Smith AL (1989b) Virus strain specificity of challenge immunity to coronavirus. Arch Virol 104:187–196CrossRefGoogle Scholar
  10. Barthold SW, Smith AL (1990) Duration of mouse hepatitis virus infection: studies in immunocompetent and chemically immunosuppressed mice. Lab Anim Sci 40:133–137PubMedGoogle Scholar
  11. Barthold SW, Smith AL (1992) Viremic dissemination of mouse hepatitis virus-JHM following intranasal inoculation of mice. Arch Virol 122:35–44PubMedCrossRefGoogle Scholar
  12. Barthold SW, Smith AL, Lord PF, Bhatt PN, Jacoby RO, Main AJ (1982) Epizootic coronaviral typhlocolitis in suckling mice. Lab Anim Sci 32:376–383PubMedGoogle Scholar
  13. Barthold SW, Beck DS, Smith AL (1988) Mouse hepatitis virus and host determinants of vertical transmission and maternally-derived passive immunity in mice. Arch Virol 100:171–183PubMedCrossRefGoogle Scholar
  14. Barthold SW, de Souza MS, Smith AL (1990) Susceptibility of laboratory mice to intranasal and contact infection with coronaviruses of other species. Lab Anim Sci 40:481–485PubMedGoogle Scholar
  15. Brownstein DG, Barthold SW (1982) Mouse hepatitis virus immunofluorescence in formalin-or Bouin’s-fixed tissues using trypsin digestion. Lab Anim Sci 32:37–39PubMedGoogle Scholar
  16. Carthew P (1981) Inhibition of the mitotic response in regenerating mouse liver during viral hepatitis. Infect Immun 33:641–642PubMedGoogle Scholar
  17. David-Ferreira JF, Manaker RA (1965) An electron microscope study of the development of a mouse hepatitis virus in tissue culture cells. J Cell Biol 24:57–78PubMedCrossRefGoogle Scholar
  18. Dupuy J, Levy-Leblond E, Le Prevost C (1975) Immuno-pathology of mouse hepatitis virus type 3 infection. II. Effect of immunosuppression in resistant mice. J Immunol 114:226–230PubMedGoogle Scholar
  19. Fujiwara K, Tamura T, Taguchi F, Hirano N, Ueda K (1977) Wasting disease in nude mice infected with facultatively virulent mouse hepatitis virus. Proceedings of the 2nd international workshop on nude mice, pp 53–60Google Scholar
  20. Hirano N, Takenaka S, Fujiwara K (1975) Pathogenicity of mouse hepatitis virus for mice depending upon host age and route of infection. Jpn J Exp Med 45:285–292PubMedGoogle Scholar
  21. Hirano N, Murakami T, Taguchi F, Fujiwara K, Matumoto M (1981) Comparison of mouse hepatitis virus strains for pathogenicity in weanling mice infected by various routes. Arch Virol 70:69–73PubMedCrossRefGoogle Scholar
  22. Ishida T, Tamura T, Ueda K, Fujiwara K (1978) Hepatosplenic myelosis in naturally occurring mouse hepatitis virus infection in the nude mouse. Nippon Juigaku Zasshi 40:739–743PubMedCrossRefGoogle Scholar
  23. Jones WA, Cohen RB (1962) The effect of murine hepatitis virus on the liver. An anatomic and histochemical study. Am J Pathol 41:329–347PubMedGoogle Scholar
  24. Lampert PW, Sims JK, Kniazeff AJ (1973) Mechanism of demyelination in JHM virus encephalomyelitis. Acta Neuropathol (Bed) 24:76–85CrossRefGoogle Scholar
  25. Le Prevost C, Levy-Leblond E, Virelizier JI, Dupuy JM (1975) Immunopathology of mouse hepatitis virus type 3 infection. Role of humoral and cell-mediated immunity in resistance mechanisms. J Immunol 114:221–225PubMedGoogle Scholar
  26. Levy-Leblond E, Dupuy JM (1977) Neonatal susceptibility to MHV 3 infection in mice. I. Transfer of resistance. J Immunol 118:1219–1222PubMedGoogle Scholar
  27. Piazza M (1969) Experimental viral hepatitis. Thomas, SpringfieldGoogle Scholar
  28. Ruebner BH, Hirano T, Slusser RJ (1967) Electron microscopy of the hepatocellular and Kupffer-cell lesions of mouse hepatitis, with particular reference to the effect of cortisone. Am J Pathol 51:163–189PubMedGoogle Scholar
  29. Smith AL (1983) An immunofluorescence test for detection of serum antibody to rodent coronaviruses. Lab Anim Sci 33:157–160PubMedGoogle Scholar
  30. Smith AL, Winograd DF (1986) Two enzyme immunoassays for the detection of antibody to rodent coronaviruses. J Virol Methods 14:335–343PubMedCrossRefGoogle Scholar
  31. Stohlman SA, Weiner LP (1981) Chronic central nervous system demyelination in mice after JHM virus infection. Neurology 31:38–44PubMedGoogle Scholar
  32. Svoboda D, Nielson A, Werder A, Higginson J (1962) An electron microscopic study of viral hepatitis in mice. Am J Pathol 41:205–224PubMedGoogle Scholar
  33. Taguchi F. Hirano N, Kiuchi Y, Fujiwara K (1976) Difference in response to mouse hepatitis virus among susceptible mouse strains. Jpn J Microbial 20:293–302Google Scholar
  34. Taguchi F, Yamada A, Fujiwara K (1979) Factors involved in the age-dependent resistance of mice infected with low-virulence mouse hepatitis virus. Arch Virol 62:333–340PubMedCrossRefGoogle Scholar
  35. Tamura T, Taguchi F, Ueda K, Fujiwara K (1977) Persistent infection with mouse hepatitis virus of low virulence in nude mice. Microbial Immunol 21:683–691Google Scholar
  36. Tardieu M, Hery C, Dupuy JM (1980) Neonatal susceptibility to MHV3 infection in mice. II. Role of natural effecter marrow cells in transfer of resistance. J Immunol 124:418–423PubMedGoogle Scholar
  37. Ward JM, Collins MJ Jr, Parker JC (1977) Naturally occurring mouse hepatitis virus infection in the nude mouse. Lab Anim Sci 27:372–376PubMedGoogle Scholar

Copyright information

© Springer-Verlag Berlin Heidelberg 1997

Authors and Affiliations

  • Stephen W. Barthold

There are no affiliations available

Personalised recommendations