Characterization of Membrane Mimetic Systems with Fluorescence

  • M. Hink
  • A. J. W. G. Visser
Chapter

Abstract

One of our major goals in signal transduction research is to understand how a living cell processes information in response to external stimuli leading ultimately to proliferation, differentiation or apoptosis. The plasma membrane has a crucial role in this process which is reflected by the fact that membrane interactions involving proteins and lipid cofactors are continuously modulated (switched off, turned on) enabling transmission of signals at the right moment and along the correct pathway. To increase our understanding of the complexity of the signal transduction network in living cells, experimental data on spatial- temporal organization of the signalling partners are required. Because of their sensitivity, microspectroscopic techniques are the methods of choice since they provide quantitative information on molecular interactions and dynamic events involving signalling molecules carrying fluorescent probes.

Keywords

Diffusion Time Fluorescence Correlation Spectroscopy Rotational Correlation Time Detergent Molecule Autocorrelation Curve 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.
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Copyright information

© Springer-Verlag Berlin Heidelberg 1999

Authors and Affiliations

  • M. Hink
  • A. J. W. G. Visser

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