Quantification on the LightCycler

  • Randy Rasmussen


Over the last 15 years, PCR has become an essential part of most laboratories involved in biomedical research. PCR amplification turns a few attograms of a specific fragment of nucleic acid (far too little to be analyzed directly or used in biochemical reactions) into as much as a microgram of DNA.


Human Immunodeficiency Virus Type Fluorescence Resonance Energy Transfer Noise Band Amplification Curve Threshold Line 
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Copyright information

© Springer-Verlag Berlin Heidelberg 2001

Authors and Affiliations

  • Randy Rasmussen
    • 1
  1. 1.Idaho TechnologySalt Lake CityUSA

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