Rapid Cycle Real-Time PCR pp 241-248 | Cite as
Relative Quantification of the HER2/neu Oncogene Using SYBR Green I
Chapter
Abstract
The HER2/neu oncogene is amplified in 10%–34% of breast tumors [1]. HER2/neu gene amplification has been correlated to poor patient prognosis and resistance to conventional therapies [2]. Current methods for detection of HER2/neu amplification include immunohistochemistry (IHC), fluorescence in-situ hybridization (FISH), southern blotting, and quantitative polymerase chain reaction (PCR). Quantitative PCR can be performed easily and rapidly on the LightCycler, making it an excellent alternative to the other methods of quantification.
Keywords
Gene Amplification Relative Quantification Control Cell Line Amplification Curve Poor Patient Prognosis
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