Cell-Free Translation Systems pp 157-163 | Cite as
Cell-Free Expression of Soluble Human Erythropoietin
Abstract
Erythropoietin (EPO) is a haematopoietic hormone produced by the kidney and secreted into the bloodstream to stimulate self-renewal and differentiation of late erythroid precursor cells toward mature red blood cells (Graber and Krantz 1978). As with many circulating hormones, EPO is highly glycosylated at conserved sites. It contains four complex carbohydrate chains, which have been implicated in the biological activity and stabilization of the protein (Goldwasser et al. 1974, Takeuchi and Kobata 1991). A possible role of these sugar chains for the correct folding and the solubility of EPO has been proposed, but this glycosylation is not necessary for binding of EPO to the specific EPO receptor (Delorme et al. 1992).
Keywords
Fusion Partner Maltose Binding Protein Sugar Chain Recombinant Chinese Hamster Ovary Cell Rapid Translation SystemPreview
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