Data-Controlled Micro-Scale Liquid Chromatography — Tandem Mass Spectrometry of Peptides and Proteins: Strategies for Improved Sensitivity, Efficiency and Effectiveness

  • Douglas C. Stahl
  • Terry D. Lee
Chapter
Part of the Principles and Practice book series (PRINCIPLES)

Abstract

Over the past decade, electrospray mass spectrometry (ESMS) has emerged as one of the most useful analytical tools for characterising peptides and proteins. As originally conceived, it was a technique that performed best at flow rates of a few microlitres per minute. Such flow rates are significantly lower than those utilised in standard analytical [4.6-mm internal diameter (ID) column] and microbore (2.1-mm ID column) chromatography. As a result, a flurry of activity ensued on the part of mass spectrometer manufacturers in order to design electrospray (ES) interfaces that would accommodate higher flow rates.

Keywords

Ally Topic Charged Droplet Sheath Liquid Capillary Liquid Chromatography Peak Parking 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.
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Copyright information

© Springer-Verlag Berlin Heidelberg 2001

Authors and Affiliations

  • Douglas C. Stahl
  • Terry D. Lee

There are no affiliations available

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