Nucleosome Assembly and Remodeling
Packaging of the eukaryotic genome into chromatin functions not only to constrain the genome within the boundaries of the cell nucleus but also to permit dynamic and broad-ranging changes related to many important biological phenomena. Therefore, chromatin assembly is a process that affects DNA replication, repair, and gene expression. Chromatin structure is linked to transcriptional regulation, and recent studies show how chromatin is altered so as to facilitate transcription. In addition, modification of chromatin structure is an important regulatory mechanism. Here I review the mechanism of chromatin assembly in vitro and the changes of chromatin structure related to transcriptional activation.
Unable to display preview. Download preview PDF.
- Asaharal H, Tartare-Deckertl S, Nakagawa T, Ikehara T, Hirose F, Hunter T, Ito T, Montminy M (2002) Dual roles of p300 in chromatin assembly and transcriptional activation in cooperation with NAP-l in vitro. Mol Cell Biol (in press)Google Scholar
- Ikeda K, Steger DJ, Eberharter A, Workman JL (1999) Activation domain-specific and general transcription stimulation by native histone acetyltransferase complexes. Mol Cell BioI 19:855–863Google Scholar
- Kamakaka RT, Bulger M, Kaufman PD, Stillman B, Kadonaga JT (1996) Postreplicative chromatin assembly by Drosophila and human chromatin assembly factor 1. Mol Cell BioI 16:810–817Google Scholar
- Kleinschmidt JA, Seiter A, Zentgraf H (1990) Nucleosome assembly in vitro: separate histone transfer and synergistic interaction of native histone complexes purified from nuclei of Xenopus laevi s oocytes. EMBOJ 9:1309–1318Google Scholar
- Kraus WL, Manning ET, Kadonaga JT (1999) Biochemical analysis of distinct activation functions in p300 that enhance transcription initiation with chromatin templates. Mol Cell BioI 19:8123–8135Google Scholar
- Pfaffle P, Jackson V (1990) Studies on rates of nucleosome formation with DNA under stress. J Biol Chern 265:16821–16829Google Scholar
- Sahasrabuddhe CG, Van Holde KE (1974) The effect of trypsin on nuclease-resistant chromatin fragments. J BioI Chern 249:152–156Google Scholar
- Van Holde KE, Lohr DE, Robert C (1992) What happens to nucleosomes during transcription? J BioI Chern 267:2837–2840Google Scholar