Exogenous Porcine Viruses

  • P. S. Paul
  • P. Halbur
  • B. Janke
  • H. Joo
  • P. Nawagitgul
  • J. Singh
  • S. Sorden
Part of the Current Topics in Microbiology and Immunology book series (CT MICROBIOLOGY, volume 278)


Porcine organs, cells and tissues provide a viable source of transplants in humans, though there is some concern of public health risk from adaptation of swine infectious agents in humans. Limited information is available on the public health risk of many exogenous swine viruses, and reliable and rapid diagnostic tests are available for only a few of these. The ability of several porcine viruses to cause transplacen126 P. S. Paul et al.tal fetal infection (parvoviruses, circoviruses, and arteriviruses), emergenceor recognition of several new porcine viruses during the last two decades (porcine circovirus, arterivirus, paramyxoviruses, herpesviruses, and porcine respiratory coronavirus) and the immunosuppressed state of the transplant recipients increases the xenozoonoses risk of humans to porcine viruses through transplantation. Much of this risk can be eliminated with vigilance and sustained monitoring along with a better understanding of pathogenesis and development of better diagnostic tests. In this review we present information on selected exogenous viruses, highlighting their characteristics, pathogenesis of viral infections in swine, methods for their detection, and the potential xenozoonoses risk they present. Emphasis has been given in this review to swine influenza virus, paramyxovirus (Nipah virus, Menagle virus, LaPiedad paramyxovirus, porcine paramyxovirus), arterivirus (porcine reproductive and respiratory syndrome virus) and circovirus as either they represent new swine viruses or present the greatest risk. We have also presented information on porcine parvovirus, Japanese encephalitis virus, encephalomyocarditis virus, herpesviruses (pseudorabies virus, porcine lymphotropic herpesvirus, porcine cytomegalovirus), coronaviruses (TGEV,PRCV, HEV, PEDV) and adenovirus. The potential of swine viruses to infect humans needs to be assessed in vitro and in vivo and rapid and more reliable diagnostic methods need to be developed to assure safe supply of porcine tissues and cells for xenotransplantation.


Swine Influenza Virus Respiratory Syndrome Virus Japanese Encephalitis Virus Nipah Virus Postweaning Multisystemic Wasting Syndrome 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.


  1. Acland HM and Littlejohns IR (1975) Encephalomyocarditis virus infection of pigs. I. An outbreak in New South Wales. Aust Vet J 51:409–415PubMedCrossRefGoogle Scholar
  2. Albrecht JC, Nicholas J, Biller D, Cameron KR, Biesinger B, Newman C, Wittmann S, Craxton MA, Coleman H, et al. (1992) Primary structure of the herpesvirus saimiri genome. J Virol 66:5047–58PubMedGoogle Scholar
  3. Allan GM and Ellis JA (2000) Porcine circoviruses: a review. J Vet Diagn Invest 12:3–14PubMedCrossRefGoogle Scholar
  4. Allan GM, Phenix KV, Todd D and McNulty MS (1994) Some biological and physicochemical properties of porcine circovirus. Zentralbl Veterinarmed [B] 41:17–26Google Scholar
  5. Allan GM, McNeilly F, Cassidy JP, Reilly GA, Adair B, Ellis WA and McNulty MS (1995) Pathogenesis of porcine circovirus, experimental infections of colostrums deprived piglets and examination of pig fetal material. Vet Microbiol 44:49–64PubMedCrossRefGoogle Scholar
  6. Allan GM, Kennedy S, McNeilly F, Foster JC, Ellis JA, Krakowka SJ, Meehan B M and Adair BM (1999) Experimental reproduction of severe wasting disease by co-infection of pigs with porcine circovirus and porcine parvovirus. J Comp Pathol 121:1–11PubMedCrossRefGoogle Scholar
  7. Allan GM, McNeilly F, Ellis J, Krakowka S, Meehan B, McNair I, Walker I and Kennedy S (2000) Experimental infection of colostrum deprived piglets with porcine circovirus 2 (PCV2) and porcine reproductive and respiratory syndrome virus (PRRSV) potentiates PCV2 replication. Arch Virol 145:2421–9PubMedCrossRefGoogle Scholar
  8. Anusz Z, Szweda W, Popkko J and Trybala E (1992) Is Aujeszky¡¯s disease a zoonosis? Przegl Epidemiol 46:181–186PubMedGoogle Scholar
  9. Bach FH, Fishman JA, Daniels N, Proimos J, Anderson B, Carpenter CB, Forrow L, Robson SC and Fineberg HV (1998) Uncertainty in xenotransplantation: individual benefit versus collective risk [see comments]. Nat Med 4:141–4PubMedCrossRefGoogle Scholar
  10. Benfield DA, Nelson E, Collins JE, Harris L, Goyal SM, Robison D, Christianson WT, Morrison RB and Gorcyca D (1992) Characterization of swine infertility and respiratory syndrome (SIRS) virus (isolate ATCC VR-2332). J Vet Diagn Invest 4:127–133PubMedCrossRefGoogle Scholar
  11. Betts AO, Jennings AR, Lamont PH and Page Z (1962) Inoculation of pigs with adenoviruses of man. Nature 193:45–46PubMedCrossRefGoogle Scholar
  12. Bolin SR, Stoffregen WC, Nayar GP and Hamel AL (2001) Postweaning multisystemic wasting syndrome induced after experimental inoculation of cesarean-derived, colostrum-deprived piglets with type 2 porcine circovirus. J Vet Diagn Invest 13:185–94PubMedCrossRefGoogle Scholar
  13. Botner A, Strandbygaard B, Sorensen KJ, Have P, Madsen KG, Madsen S and Alexandersen S (1997) Appearance of acute PRRS-like symptoms in sow herds after vaccination with a modified live PRRS vaccine. Vet Rec 141:497–499PubMedCrossRefGoogle Scholar
  14. Brown IH (2000) The epidemiology and evolution of influenza viruses in pigs. Vet Microbiol 74:29–46PubMedCrossRefGoogle Scholar
  15. Brown IH, Done SH, Spencer YI, Cooley WA, Harris PA and Alexander DJ (1993) Pathogenicity of a swine influenza H1N1 virus antigenically distinguishable from classical and European strains. Vet Rec 132:598–602PubMedCrossRefGoogle Scholar
  16. Brown IH, Chakraverty P, Harris PA and Alexander DJ (1995) Disease outbreaks in pigs in Great Britain due to an influenza A virus of H1N2 subtype. Vet Rec 136:328–329PubMedCrossRefGoogle Scholar
  17. Burke DS, Tingpalapong M, Elwell MR, Paul PS, Van Deusen RA. (1985) Japanese ncephalitis virus immunoglobulin M antibodies in porcine sera. Am J Vet Res 46:2054–2057PubMedGoogle Scholar
  18. Callebaut P, Pensaert MB and Hooyberghs J (1989) A competitive inhibition ELISA for the differentiation of serum antibodies from pigs infected with transmissible gastroenteritis virus (TGEV) or with the TGEV-related porcine respiratory coronavirus. Vet Microbiol 20:9–19PubMedCrossRefGoogle Scholar
  19. Chan KP, Rollin PE, Ksiazek TG, Leo YS, Goh KT, Paton NI, Sng EH, Ling AE (2002) A survey of Nipah virus infection among various risk groups in Singapore. Epidemiol Infect 128:93–98PubMedCrossRefGoogle Scholar
  20. Chant K, Chan R, Smith M, Dwyer DE, Kirkland P and the NSW Expert Group (1998) Probable human infection with a newly described virus in the family Paramyxoviridae. The NSW Expert Group Emerg Infect Dis 4:273–5CrossRefGoogle Scholar
  21. Chapman LE, Folks TM, Salomon DR, Patterson AP, Eggerman TE and Noguchi PD (1995) Xenotransplantation and xenogeneic infections. N Engl J Med 333:1498–501PubMedCrossRefGoogle Scholar
  22. Chew MHL, Arguin PM, Shay DK, Goh KT, Rollin PE, Shieh WJ, Zaki SR, Rota PA, Ling AE, Ksiazek TG, Chew SK and Anderson LA (2000) Risk factors for Nipah virus infection among abattoir workers in Singapore. J Infect Dis 181:1760–1763PubMedCrossRefGoogle Scholar
  23. Christianson WT, Kim HS, Yoon IJ and Joo HS (1992) Transplacental infection of midgestation sows with encephalomyocarditis virus. Am J Vet Res 53:44–47PubMedGoogle Scholar
  24. Christopher-Hennings J, Nelson EA, Nelson JA, Hines RJ, Swenson SL, Hill HT, Zimmerman JJ, Katz JB, Yaeger MJ, Chase CCL and Benfield DA (1995) Detection of porcine reproductive and respiratory syndrome virus in boar semen by PCR. J Clin Microbiol 33:1730–1734PubMedGoogle Scholar
  25. Chua KB, Bellini WJ, Rota PA, Harcourt BH, Tamin A, Lam SK, Ksiazek TG, Rollin PE, Zaki SR, Shieh WJ, Goldsmith CS, Gubler DJ, Roehrig JT, Eaton B, Gould AR, Olson J, Field H, Daniels P, Ling AE, Peters CJ, Anderson LJ and Mahy BWJ (2000a) Nipah virus: a recently emergent deadly paramyxovirus. Science 288:1432–1435CrossRefGoogle Scholar
  26. Chua KB, Goh KJ, Wong KT, Kamarulzaman A, Tan PSK, Ksiazek TG, Zaki SR, Paul G, Lam SK and Tan CT (2000b) Fatal encephalitis due to Nipah virus among pigfarmers in Malaysia. Lancet 354:1257–1259CrossRefGoogle Scholar
  27. Claas ECJ, Kawaoka Y, de Jong JC, Masurel N and Webster RG (1994) Infection of children with avian-human reassortant influenza virus from pigs in Europe. Virology 204:453–457PubMedCrossRefGoogle Scholar
  28. Collins JE, Benfield DA, Christianson WT, Harris L, Hennings JC, Shaw DP, Goyal SM, McCullough S, Morrison RB and Joo HS (1992) Isolation of swine infertility and respiratory syndrome virus (isolate ATCC VR-2332) in North America and experimental reproduction of the disease in gnotobiotic pigs. J Vet Diagn Invest 4:117–126PubMedCrossRefGoogle Scholar
  29. Croghan DL, Matchett A and Koski TA (1973) Isolation of porcine parvovirus from commercial trypsin. Appl Microbiol 26:431–433PubMedGoogle Scholar
  30. Daniels P (1999) Experimental infection of pigs and cats at CSIRO-AAHL-Preliminary Observations. A working paper for WHO Meeting on Zoonotic Paramyxoviruses, Kuala Lumpur, Malaysia, 19–21 July, 1999Google Scholar
  31. Done JT (1955) An“inclusion body”rhinitis of pigs. Vet Record 70:877–878Google Scholar
  32. Dulac GC and Afshar A (1989) Porcine circovirus antigens in PK-15 cell line (ATCC CCL-33) and evidence of antibodies to circovirus in Canadian pigs. Can J Vet Res 53:431–3PubMedGoogle Scholar
  33. Easterday BC and Van Reeth K (1999) Swine influenza. In Straw BE, D’Allaire S, Mengeling WL, et al. (eds). Diseases of Swine. Iowa State University Press, Ames, IA. 8th ed. Pp. 277–290Google Scholar
  34. Edington N, Plowright Wand Watt RG (1976a) Generalized porcine cytomegalic inclusion disease: distribution of cytomegalic cells and virus. J Comp Pathol 86:191–202CrossRefGoogle Scholar
  35. Edington N, Watt RG and Plowright W (1976b) Cytomegalovirus excretion in gnotobiotic pigs. J Hyg (Camb) 77:283–290CrossRefGoogle Scholar
  36. Edington N, Watt RG and Plowright W (1977) Experimental transplacental transmission of porcine cytomegalovirus. J Hyg (Lond) 78:243–51CrossRefGoogle Scholar
  37. Edington N (1999) Cytomegalovirus. In Straw B, D_allaire S, Mengeling WL, et al. (Eds) Diseases of Swine, Iowa State University Press, Ames, Ia 8th Edition pp 125–131Google Scholar
  38. Ehlers B, Ulrich S and Goltz M (1999) Detection of two novel porcine herpesviruses with high similarity to gammaherpesviruses. J Gen Virol 80:971–8PubMedGoogle Scholar
  39. Ellis J, Hassard L, Clark E, Harding J, Allan G, Willson P, Strokappe J, Martin K, McNeilly F, Meehan B, Todd D and Haines D (1998) Isolation of circovirus from lesions of pigs with postweaning multisystemic wasting syndrome. Can Vet J 39:44–51PubMedGoogle Scholar
  40. Ellis JA, Bratanich A, Clark EG, Allan G, Meehan B, Haines DM, Harding J, West KH, Krakowka S, Konoby C, Hassard L, Martin K and McNeilly F (2000) Coinfection by porcine circoviruses and porcine parvovirus in pigs with naturally acquired postweaning multisystemic wasting syndrome. J Vet Diagn Invest 12:21–7PubMedCrossRefGoogle Scholar
  41. Ensser A, Pflanz R and Fleckenstein B. (1997) Primary structure of the alcelaphine herpesvirus 1 genome. J Virol 71:6517–25PubMedGoogle Scholar
  42. Erdman DD, Evatt BL, Anderson LJ, Torok TJ, Jamil M, Barnhart E, Tepper M, Burrill HN, Pickett AM, Mengeling WL (2000) Investigation of porcine parvovirus among persons with hemophilia receiving Hyate:C porcine factor VIII concentrate. Transfusion-Bethesda 40:708–711Google Scholar
  43. Fenaux M, Halbur PG, Gill M, Toth TE and Meng XJ (2000) Genetic characterization of type 2 porcine circovirus (PCV-2) from pigs with postweaning multisystemic wasting syndrome in different geographic regions of North America and development of a differential PCR- restriction fragment length polymorphism assay to detect and differentiate between infections with PCV-1 and PCV-2. J Clin Microbiol 38:2494–503PubMedGoogle Scholar
  44. Fenaux M, Halbur PG, Haqshenas G, Royer R, Thomas P, Nawagitgul P, Gill M, Toth TE and Meng XJ (2002) Cloned genomic DNA of type 2 porcine circovirus is infectious when injected directly into the liver and lymph nodes of pigs: Character ization of clinical disease, virus distribution, and pathologic lesions. J Virol 76:541–551PubMedCrossRefGoogle Scholar
  45. Feng WH, Laster SM, Tompkins M, Brown T, Xu JS, Altier C, Gomez W, Benfield, D and McCaw MB (2001) In utero infection by porcine reproductive and respiratory syndrome virus is sufficient to increase susceptibility of piglets to challenge with Streptococcus suis type II. J Virol 75:4889–4895PubMedCrossRefGoogle Scholar
  46. Fishman JA (1994) Miniature swine as organ donors for man: strategies for prevention of xenotransplant-associated infections. Xenotransplantation 1:47–57CrossRefGoogle Scholar
  47. Fryer-Jacqueline FL, Griifiths PD, Fishman JA, Emery VC and Clark DA (2001) Quantitation of porcine cytomegalovirus in pig tissues by PCR. J Clin Microbiol 39:1155–1156CrossRefGoogle Scholar
  48. Fujisaki Y, Sugimori T, Morimoto T, Miura Y, Kawakami Yand Nakano K (1975) Immunization of pigs with the attenuated S strain of Japanese encephalitis virus. National Institute Animal Health Quarterly (Tokyo) 15:55–60Google Scholar
  49. Gainer JH (1967) Encephalomyocarditis virus infection in Florida, 1960–1966. J Am Vet Med Assoc 151:1960–1966PubMedGoogle Scholar
  50. Goh KJ, Tan CT, Chew NK, Tan PSK, Kamarulzaman A, Sarji SA, Wong KT, Abdullah BJJ, Cua KB and Lam SK (2000) Clinical features of Nipah virus encephalitis among pig farmers in Malaysia. The New England J Med 342:1229–1235CrossRefGoogle Scholar
  51. Goldfield M, Noble G and Dowdle WR (1977) Identification and preliminary antigenic analysis of swine influenza-like viruses isolated during an influenza outbreak at Fort Dix, New Jersey. J Infect Dis 136 (Suppl):381–385CrossRefGoogle Scholar
  52. Gourreau JM, Kaiser C, Valette M, Douglas AR, Labie J and Aymard M (1994) Isolation of two H1N2 influenza viruses from swine in France. Arch Virol 135:365–382PubMedCrossRefGoogle Scholar
  53. Greig AS, Johnson CM and Bovillant AMP (1971) Encephalomyelitis of swine caused by a hemagglutinating virus. VI. Morphology of the virus. Res Vet Sci 12:305–307PubMedGoogle Scholar
  54. Groshup MH, Brun A and Haas B (1993) Serological studies on the potential synergism of porcine respiratory and reproductive syndrome virus and influenza-, corona-, and paramyxoviruses in the induction of respiratory syndrome in swine. J Vet Med B 40:681–689CrossRefGoogle Scholar
  55. Gunzburg WH and Salmons B (2000) Xenotransplantation: is the risk of viral infection as great as we thought? Mol Med Today 6:1999–208CrossRefGoogle Scholar
  56. Halbur PG, Andrews JJ, Huffman EL, Paul PS, Meng XJ and Niyo Y (1994) Development of a streptoavidin-biotin immunoperoxidase procedure for the detection of porcine reproductive and respiratory syndrome virus antigen in porcine lung. J Vet Diagn Invest 6:254–257PubMedCrossRefGoogle Scholar
  57. Halbur PG, Paul PS, Frey ML, Landgraf J, Eernisse K, Meng X-J, Lum MA, Andrews JJ and Rathje JA (1995) Comparison of the pathogenicity of two U.S. porcine reproductive and respiratory syndrome virus isolates with that of the Lelystad virus. Vet Pathol 32:648–660PubMedCrossRefGoogle Scholar
  58. Hamel AL, Lin LL and Nayar GP (1998) Nucleotide sequence of porcine circovirus associated with postweaning multisystemic wasting syndrome in pigs. J Virol 72:5262–7PubMedGoogle Scholar
  59. Hamel AL, Lin L, Sachvie C, Grudeski E and Nayar GPS (1999) PCR assay for detecting porcine cytomegalovirus. J Clin Microbiol 37:3767–8PubMedGoogle Scholar
  60. Harms PA, Sorden SD, Halbur PG, Bolin SR, Lager KM, Morozov I and Paul PS (2001) Experimental reproduction of severe disease in CD/CD pigs concurrently infected with type 2 porcine circovirus and porcine reproductive and respiratory syndrome virus. Vet Pathol 38:528–39PubMedCrossRefGoogle Scholar
  61. Harcourt BH, Tamin A, Ksiazek TG, Rollin PE, Anderson LJ, Bellini WJ and Rota PA (2000) Molecular characterization of Nipah virus, a newly emergent paramyxovirus. Virology 271:334–349PubMedCrossRefGoogle Scholar
  62. Harding JC and Clark EG (1997) Recognizing and diagnosing postweaning multisystemic wasting syndrome (PMWS). Swine Health and Production 5:201–203Google Scholar
  63. Harding JCS, Clark EG, Strokappe JH, Willson PI and Ellis JA (1998) Post-weaning multisystemic wasting syndrome (PMWS): epidemiology and clinical presentation. Swine Health Prod 6:249–254Google Scholar
  64. Hay AJ (1998) The virus genome and its replication. In Nicholson KG, Webster RG, Hay AJ (eds), Textbook of Influenza. Blackwell Science, Oxford pp. 43–53Google Scholar
  65. Haynes JS, Halbur PG, Sirinarumitr T, Paul PS, Meng XJ and Huffman EL (1997) Temporal and morphologic characterization of the distribution of porcine reproductive and respiratory syndrome virus (PRRSV) by in situ hybridization in pigs infected with isolates of PRRSV that differ in virulence. Vet Pathol 34:39–43PubMedCrossRefGoogle Scholar
  66. Heinen E, Herbst Wand Schmeer N (1998) Isolation of cytopathogenic virus from a case of porcine respiratory and reproductive syndrome (PRRS) and its characterization as parainfluenza virus type 2. Arch Virol 143:2233–2239PubMedCrossRefGoogle Scholar
  67. Hill H (1990) Overview and history of mystery swine disease (swine infertility and respiratory syndrome). Proc. Mystery Swine Dis Committee Mtg, October 6, Denver, CO, pp. 29–31. Livestock Conservation Institute, Madison, WIGoogle Scholar
  68. Hinshaw VS, Bean WJ jr, Webster RG and Easterday BC (1978) The prevalence of influenza viruses in swine and the antigenic and genetic relatedness of influenza viruses of man and swine. Virology 84:51–62PubMedCrossRefGoogle Scholar
  69. Hooper P, Zaki S, Daniels P and Middleton D (2001) Comparative pathology of the diseases caused by Hendra and Nipah viruses. Microbes and Infection 34:323–329Google Scholar
  70. Hori H, Morita K and Igarashi A (1986) Oligonucleotide fingerprint analysis on Japanese encephalitis virus strains isolated in Japan and Thailand. Acta Virol 30:353–359PubMedGoogle Scholar
  71. Hsu ST, Chang LC, Lin SY, Chuang TY, Ma CH, Inoue YK and Okuno T (1972) The effect of vaccination with a live attenuated strain of Japanese encephalitis virus on stillbirths in swine in Taiwan. Bulletin WHO 46:465–471Google Scholar
  72. Janke BH (2000) Diagnosis of swine influenza. Swine Health Prod 8:79–84Google Scholar
  73. Janke BH, Paul PS, Landgraf JG, Halbur PG and Huinker CD (2001) Paramyxovirus infection in pigs with interstitial pneumonia and encephalitis in the United States. J Vet Diagn Invest 13:428–433PubMedCrossRefGoogle Scholar
  74. Joo HS, Kim HS and Leman AD (1988) Detection of antibody to encephalomyocarditis virus in mummified or stillborn pigs. Arch Virol 100:131–134PubMedCrossRefGoogle Scholar
  75. Josephson G and Charbonneau G (2001) Case report of reproductive failure in a new startup operation. J Swine Health Prod 9:258–259Google Scholar
  76. Karasin AI, Olsen CW and Anderson GA (2000a) Genetic characterization of an H1N2 influenza virus isolated from a pig in Indiana. J Clin Microbiol 38:2453–2456Google Scholar
  77. Karasin AI, Brown IH, Carman S and Olsen CW (2000b) Isolation and characterization of H4N6 avian influenza viruses from pigs with pneumonia in Canada. J Virol 74:9322–9327CrossRefGoogle Scholar
  78. Kim HS, Christianson WTand Joo HS (1989) Pathogenic properties of encephalomyocarditis virus isolates in swine fetuses. Arch Virol 109:51–57PubMedCrossRefGoogle Scholar
  79. Kim J, Han DU, Choi C and Chae C (2001) Differentiation of porcine circovirus (PCV)-1 and PCV-2 in boar semen using a multiplex nested polymerase chain reaction. J Virol Methods 98:25–31PubMedCrossRefGoogle Scholar
  80. Kennedy S, Moffett D, McNeilly F, Meehan B, Ellis J, Krakowka S and Allan GM (2000) Reproduction of lesions of postweaning multisystemic wasting syndrome by infection of conventional pigs with porcine circovirus type 2 alone or in combination with porcine parvovirus. J Comp Pathol 122:9–24PubMedCrossRefGoogle Scholar
  81. Kluge JP, Beran GW, Hill HT, Platt KB (1992) Pseudorabies(Aujeszky_s Disease) In Leman AD, Straw BE, Mengeling WL, D’Allaire S, Taylor DJ (eds).Diseases of Swine, Iowa State University Press, Ames, IA. 7th ed. pp.312–323Google Scholar
  82. Krakowka S, Ellis JA, Meehan B, Kennedy S, McNeilly F and Allan G (2000) Viral wasting syndrome of swine: experimental reproduction of postweaning multisystemic wasting syndrome in gnotobiotic swine by coinfection with porcine circovirus 2 and porcine parvovirus. Vet Pathol 37:254–63PubMedCrossRefGoogle Scholar
  83. Krakowka S, Ellis JA, McNeilly F, Ringler S, Rings DM and Allan G (2001) Activation of the immune system is the pivotal event in the production of wasting disease in pigs infected with porcine circovirus-2 (PCV-2). Vet Pathol 38:31–42PubMedCrossRefGoogle Scholar
  84. Larochelle R, Antaya M, Morin M and Magar R (1999) Typing of porcine circovirus in clinical specimens by multiplex PCR. J Virol Methods 80:69–75PubMedCrossRefGoogle Scholar
  85. Larochelle R, Bielanski A, Muller P and Magar R (2000) PCR detection and evidence of shedding of porcine circovirus type 2 in boar semen. J Clin Microbiol 38:4629–4632PubMedGoogle Scholar
  86. Lipkind M, Shohan D and Shihmanter E (1986) Isolation of paramyxovirus from pigs in Israel and its antigenic relationship with avian paramyxoviruses. J Gen Virol 67:422–439CrossRefGoogle Scholar
  87. Littlejohns IR and Acland HM (1975) Encephalomyocarditis virus infection in pigs. II. Experimental diseases. Aust Vet J 51:416–422PubMedCrossRefGoogle Scholar
  88. Liu Q, Wang L, Willson P and Babiuk L A (2000) Quantitative, competitive PCR analysis of porcine circovirus DNA in serum from pigs with postweaning multisystemic wasting syndrome. J Clin Microbiol 38:3474–3477PubMedGoogle Scholar
  89. Love RJ and Grewal AS (1986) Reproductive failure in pigs caused by encephalomyocarditis virus. Aust Vet J 63:128–129PubMedCrossRefGoogle Scholar
  90. Magar R, Larochelle R, Thibault S and Lamontagne L (2000) Experimental transmission of porcine circovirus type 2 (PCV2) in weaned pigs: a sequential study. J Comp Pathol 123:258–269PubMedCrossRefGoogle Scholar
  91. McCormack JG (2000) Hendra, Menangle and Nipah viruses. Aust NZ J Med 30:9–10CrossRefGoogle Scholar
  92. Meng XJ, Paul PS, Vaughn EM and Zimmerman JJ (1993) Development of a Nucleic Acid Probe for the Detection of Encephalomyocarditis Virus of Swine. J Vet Diag Invest 5:254–258CrossRefGoogle Scholar
  93. Meng XJ, Paul PS, Halbur PG and Morozov I (1995) Sequence comparison of open reading frames 2 to 5 of low and high virulence United States isolates of porcine reproductive and respiratory syndrome virus. J gen Virol 76:3181–3188PubMedCrossRefGoogle Scholar
  94. Meng XJ, Paul PS, Halbur PG and Lum MA (1996) Characterization of a high-virulence US isolate of porcine reproductive and respiratory syndrome virus in a continuous cell line, ATCC CRL11171. J Vet Diagn Invest 8:374–381PubMedCrossRefGoogle Scholar
  95. Mengeling WL. Porcine parvovirus. (1992) In Leman AD, Straw BE, Mengeling WL, D’Allaire S, Taylor DJ (eds). Diseases of Swine. Iowa State University Press, Ames, IA. 7th edition pp. 299–311Google Scholar
  96. Mengeling WL, Vorwald AC, Lager KM and Brockmeier SL (1996) Comparison among strains of porcine reproductive and respiratory syndrome virus for their ability to cause reproductive failure. Am J Vet Res 57:834–839PubMedGoogle Scholar
  97. Miyata H, Tsunoda H, Kazi A, Yamada A, Khan MA, Murakami J, Kamahora T, Shiraki K and Hino S (1999) Identification of a novel GC-rich 113-nucleotide region to complete the circular, single-stranded DNA genome of TT virus, the first human circovirus. J Virol 73:3582–3586PubMedGoogle Scholar
  98. Morimoto T (1969) Epizootic swine stillbirth caused by Japanese encephalitis virus. Proceedings of a symposium on factors producing embryonic and fetal abnormalities, death, and abortion in swine. US ARS 91–73:91–73Google Scholar
  99. Morozov I, Sirinarumitr T, Sorden SD, Halbur PG, Morgan MK, Yoon KJ, Paul PS (1998) Detection of a novel strain of porcine circovirus in pigs with postweaning multisystemic wasting syndrome. J Clin Microbiol 36:2535–2541PubMedGoogle Scholar
  100. Murray K, Selleck P, Hooper P, Hyatt A, Gould A, Gleeson L, Westbury H, Hiley L, Selvey L and Rodwell B (1995) A morbillivirus that caused fatal disease in horses and humans. Science 268:94–97PubMedCrossRefGoogle Scholar
  101. Nawagitgul P, Morozov I, Bolin SR, Harms PA, Sorden SD and Paul PS (2000) Open reading frame 2 of porcine circovirus type 2 encodes a major capsid protein. J gen Virol 9:2281–2287Google Scholar
  102. Nawagitgul P, Harms PA, Morozov I, Thacker BJ, Sorden SD, Lekcharoensuk C and Paul PS (2002) Modified indirect porcine circovirus (PCV) type 2-based and recombinant capsid protein (ORF2)-based enzyme-linked immunosorbent assays for detection of antibodies to PCV. Clin Diagn Lab Immunol 9:33–40PubMedGoogle Scholar
  103. Nelson EA, Christopher-Hennings J, Drew T, Wensvoort G, Collins JE and Benfield DA (1993) Differentiation of U.S. and European isolates of porcine reproductive and respiratory syndrome virus by monoclonal antibodies. J Clin Microbiol 31:3184–3189PubMedGoogle Scholar
  104. Nielsen HS, Oleksiewicz MB, Forsberg R, Stadejek T, Botner A and Storgaard T (2001) Reversion of a live porcine reproductive and respiratory syndrome virus vaccine investigated by parallel mutations. J gen Virol 82:1263–1272PubMedGoogle Scholar
  105. Ogasa A, Yokoki Y, Fujisaki Y and Habu A (1977) Reproductive disorders in boars infected experimentally with Japanese encephalitis virus. Jap J Animal Reproduction 23:171–175CrossRefGoogle Scholar
  106. Ouchi A, Nerome K, Kanegae Y, Ishida M, Nerome R, Hayashi K, Hashimoto T, Kaji M, Kaji Yand Inaba Y (1996) Large outbreak of swine influenza in southern Japan caused by reassortant (H1N2) influenza viruses: Its epizootic background and characterisation of the causative viruses. J gen Virol 77:1751–1759PubMedCrossRefGoogle Scholar
  107. Parashar UD, Sunn LM, Ong F, Mounts AW, Arif MT, Ksiazek TG, Kamaluddin MA, Mustafa AN, Kaur H, Ding LM, Othman G, Radzi HM, Kitsutani PT, Stockton PC, Arokiasam J, Gary HEJr and Anderson LJ (2000) Case-control study of risk factors for human infection with a new zoonotic paramyxovirus, Nipah virus, during a 1998–1999 outbreak of severe encephalitis in Malaysia. J Infect Dis 181:1998–1999PubMedCrossRefGoogle Scholar
  108. Patience C, Takeuchi Y and Weiss RA (1997) Infection of human cells by an endogenous retrovirus of pigs [see comments]. Nature Med 3:282–286PubMedCrossRefGoogle Scholar
  109. Patience C, Takeuchi Y and Weiss RA (1998) Zoonosis in xenotransplantation. Curr Opin Immunol 10:539–542PubMedCrossRefGoogle Scholar
  110. Patriarca RA, Kendal AR, Zakowski RC, Cox NJ, Trautman MS, Cherry JD, Auervach DM, McCusker J, Belliveau RR and Kappus KD (1984) Lack of significant personto-person spread of swine infuenza-like virus following fatal infection in an immunocompromised child. Am J Epidemiol 119:152–158PubMedGoogle Scholar
  111. Paul PS, Mengeling WL and Pirtle EC (1982) Differentiation of pseudorabies virus isolates by restriction endonuclease analysis. Arch Virol 73:193–198PubMedCrossRefGoogle Scholar
  112. Paul PS, Halbur PG and Vaughn E (1994) Significance of porcine respiratory coronavirus infection. Compend Cont Educ Pract Vet 16:1223–1234Google Scholar
  113. Pensaert MB, Callebaut P and Vergote J (1986) Isolation of porcine respiratory nonenteric coronavirus related to transmissible gastroenteritis. Vet Quart 8:257–261CrossRefGoogle Scholar
  114. Philbey A, Kirkland DP, Ross A, Davis RJ, Gleeson AB, Love RJ, Daniels PW, Gould AR and Hyatt AD (1998) An apparently new virus (Family Paramyxoviridae) infectious for pigs, humans, and fruit bats. Emerging Infectious Diseases 2:269–271CrossRefGoogle Scholar
  115. Plagemann PG and Moenning B (1992) Lactate dehydrogenase-elevating virus, equine arteritis virus and simian hemorrhagic fever virus: A new group of positive stranded RNA viruses. Adv Virus Res 41:99–102PubMedCrossRefGoogle Scholar
  116. Plowright W, Edington N and Watt RG (1976) The behavior of porcine cytomegalovirus in commercial pig herds. J Hyg (Lond) 76:125–135CrossRefGoogle Scholar
  117. Royer RL, Nawagitgul P, Halbur PG and Paul PS (2001) Susceptibility of porcine circovirus type 2 to commercial and laboratory disinfectants. Swine Health Prod 9:281–284Google Scholar
  118. Rupasinghe V, Tajima T, Maeda K, Iwatsuki HK, Sugii S and Horimoto T (1999) Analysis of porcine cytomegalovirus DNA polymerase by consensus primer PCR. J Vet Med Sci 61:1253–1255PubMedCrossRefGoogle Scholar
  119. Saif LJ and Wesley RD (1999) Transmissible gastroenteritis and porcine respiratory coronavirus. In Straw BE, D’Allaire S, Mengeling WL, et al. (Eds) Diseases of Swine, Iowa State University Press, Ames, IA 8th edition pp 295–325Google Scholar
  120. Sasahara J, Hayashi S, Kumagai T, Yamamoto Y, Hirasawa K, Munekata K, Okaniwa A and Kato K (1954) On a swine virus disease newly discovered in Japan. 1. Isolation of the virus. 2. Some properties of the virus. Virus 4:131–139CrossRefGoogle Scholar
  121. Shimizu T, Kawakami Y, Fukuhaira S and Matsumoto M (1954) Experimental stillbirth in pregnant swine infected with Japanese encephalitis virus. Jap J Exp Med 24:363–375PubMedGoogle Scholar
  122. Sirinarumitr T, Paul PS, Kluge JP and Halbur PG (1996) In situ hybridization technique for the detection of swine enteric and respiratory coronaviruses, transmissible gastroenteritis virus (TGEV) and porcine respiratory coronavirus (PRCV), in formalin-fixed paraffin-embedded tissues. J Virol Methods 56:149–160PubMedCrossRefGoogle Scholar
  123. Sirinarumitr T, Paul PS, Halbur PG and Kluge JP (1997) An overview of immunological and genetic methods for detecting swine coronaviruses, transmissible gastroenteritis virus, and porcine respiratory coronavirus in tissues. Mechanisms in the pathogenesis of enteric diseases ed. Paul, Francis and Benfield. Plenum Press, New York, pp. 37–46Google Scholar
  124. Sirinarumitr T, Zhang Y, Kluge JP, Halbur PG and Paul PS (1998) A pneumo-virulent United States isolate of porcine reproductive and respiratory syndrome virus induces apoptosis in bystander cells both in vitro and in vivo. J gen Virol 79:2989–2995PubMedGoogle Scholar
  125. Sirinarumitr T, Morozov I, Nawagitgul P, Sorden SD, Harms PA and Paul PS (2000) Utilization of a rate enhancement hybridization buffer system for rapid in situ hybridization for the detection of porcine circovirus in cell cultures and in tissues of pigs with postweaning multisystemic wasting syndrome (PMWS). J Vet Diagn Invest 12:562–565PubMedCrossRefGoogle Scholar
  126. Sorden SD, Harms PA, Nawagitgul P, Cavanaugh D and Paul PS (1999) Development of a polyclonal-antibody-based immunohistochemical method for the detection of type 2 porcine circovirus in formalin-fixed, paraffin-embedded tissue. J Vet Diagn Invest 11:528–530PubMedCrossRefGoogle Scholar
  127. Sorden SD (2000) Update on porcine circovirus and postweaning multisystemic wasting syndrome (PMWS). Swine Health Prod 8:133–136Google Scholar
  128. Stephano HA (1999) Blue eye disease. In Diseases of swine, 8th edition, editors B. Straw, S. Allaire, W.Mengeling, D.Taylor. Iowa State University Press, Ames, Iowa, pp. 103–112Google Scholar
  129. Stephano HA, Gay GM and Ramirez TC (1988) Encephalomyelitis, reproductive failure and corneal opacity (blue eye) in pigs, associated with a paramyxovirus infection. Vet Record 122:6–10CrossRefGoogle Scholar
  130. Stevenson GW, Kiupel M, Mittal SK, Choi J, Latimer KS and Kanitz CL (2001) Tissue distribution and genetic typing of porcine circoviruses in pigs with naturally occurring congenital tremors. J Vet Diagn Invest 13:57–62PubMedCrossRefGoogle Scholar
  131. Takashima I, Watanabe T, Ouchi N and Hashimoto N (1988) Ecological studies of Japanese encephalitis virus in Hokkaido: Inter-epidemic outbreaks of swine abortion and evidence for the virus to over winter locally. Am J Tropical Med Hygiene 38:420–427Google Scholar
  132. Tchoyoson-Lim CC, Sitoh YY, Hui F, Lee KE, Ang BSP, Lim E, Lim WEH, Oh HML, Tambyah PA, Wong JSL, Tan CB and Chee TSG (2000) Nipah viral encephalitis or Japanese encephalitis? MR findings in a new zoonotic disease. Am J Neurological Res 21:455–461Google Scholar
  133. Tesh RB (1978) The prevalence of encephalomyocarditis virus neutralizing antibodies among various human populations. Am J Tropical Med Hygiene 27:144–149Google Scholar
  134. Tischer I, Rasch R and Tochtermann G (1974) Characterization of papovavirus-and picornavirus-like particles in permanent pig kidney cell lines. Zentralbl Bakteriol Mikrobiol Hyg [A]. 226:153–167Google Scholar
  135. Tischer I, Gelderblom H, Vettermann W and Koch MA (1982) A very small porcine virus with circular single-stranded DNA. Nature 295:64–6PubMedCrossRefGoogle Scholar
  136. Tischer I, Mields W, Wolff D, Vagt M and Griem W (1986) Studies on epidemiology and pathogenicity of porcine circovirus. Arch Virol 91:271–6PubMedCrossRefGoogle Scholar
  137. Tischer I, Peters D, Rasch R and Pociuli S (1987) Replication of porcine circovirus: induction by glucosamine and cell cycle dependence. Arch Virol 96:39–57PubMedCrossRefGoogle Scholar
  138. Tischer I, Bode L, Apodaca J, Timm H, Peters D, Rasch R, Pociuli S and Gerike E (1995) Presence of antibodies reacting with porcine circovirus in sera of humans, mice, and cattle. Arch Virol 140:1427–39PubMedCrossRefGoogle Scholar
  139. Ulrich S, Goltz M and Ehlers B (1999) Characterization of the DNA polymerase loci of the novel porcine lymphotropic herpesviruses 1 and 2 in domestic and feral pigs. J Gen Virol 80:3199–3205PubMedGoogle Scholar
  140. Umene K (1999) Mechanism and application of genetic recombination in herpesviruses. Rev Med Virol 9:171–182PubMedCrossRefGoogle Scholar
  141. Underdahl NR, Mebus CA, Stair EL and Twiehaus MJ (1975) Recovery of transmissible gastroenteritis virus from chronically infected experimental pigs. Am J Vet Res 36:1473–1476PubMedGoogle Scholar
  142. Van Reeth K, Brown IH and Pensaert M (2000) Isolations of H1N2 influenza A virus from pigs in Belgium. Vet Rec 146:588–589PubMedCrossRefGoogle Scholar
  143. van-Vugt-Joke JFA, Storgaard T, Oleksiewicz MB and Botner A. (2001) High frequency RNA recombination in porcine reproductive and respiratory syndrome virus occurs preferentially between parental sequences with high similarity. J gen Virol 2:2615–2620Google Scholar
  144. Vaughn EM, Halbur PG and Paul PS (1995) Sequence comparison of porcine respiratory coronavirus isolates reveals heterogenecity in the S, 3, 3–1 genes. J Virol 69:3–1PubMedGoogle Scholar
  145. Vincent LL, Janke BH, Paul PS and Halbur PG (1997) A monoclonal-antibody-based immunohistochemical method for the detection of swine influenza virus in formalin-fixed, paraffin embedded tissues. J Vet Diagn Invest 9:191–195PubMedCrossRefGoogle Scholar
  146. Walker IW, Konoby CA, Jewhurst VA, McNair I, McNeilly F, Meehan BM, Cottrell TS, Ellis JA, Allan GM (2000) Development and application of a competitive enzymelinked immunosorbent assay for the detection of serum antibodies to porcine circovirus type 2. J Vet Diagn Invest 12: 400–405PubMedCrossRefGoogle Scholar
  147. Virgin HW, Latreille P, Wamsley P, Hallsworth K, Weck KE, Dal-Canto AJ and Speck SH (1997) Complete sequence and genomic analysis of murine gammaherpesvirus 68. J Virol 71:5894–904PubMedGoogle Scholar
  148. Wang LF, Yu M, Hansson E, Pritchard LI, Shiell B, Michalski WP and Eaton BT (2000) The exceptional large genome of Hendra virus: support for creation of a new genus within the family Paramyxoviridae. J Virol 74:9972–9979PubMedCrossRefGoogle Scholar
  149. Weiss RA (1998) Transgenic pigs and virus adaptation. Nature 391:327–8PubMedCrossRefGoogle Scholar
  150. Wensvoort G, Terpstra C, Pol JM, TerLaak EA, Bloemradd M, DeKluyver EP, Kragten C, Van Butten L, Den BEsten A, Wagenaar F, Broekhuijsen JM, Moonen PLJM, Zetstra T, DeBoer EA, Tibben HJ, De Jong MF, Vanit Veld P, Groenland GJR, Van Gennep JA, Voets MT, Verheijden JHM and Braamskamp J (1991) Mystery swine disease in the Netherlands: the isolation of Lelystad virus. Veterinary Quarterly 13:121–130PubMedCrossRefGoogle Scholar
  151. Wentworth DE, Thompson BL, Xu X, Regnery HL, Cooley AJ, McGregor MW, Cox NJ and Hinshaw VS (1994) An influenza A (H1N1) virus, closely related to swine influenza virus, responsible for a fatal case of human influenza. J Virol 68:2051–2058PubMedGoogle Scholar
  152. Wentworth DE, McGregor MW, Macklin MD, Neumann V and Hinshaw VS (1997) Transmission of swine influenza virus to humans after exposure to experimentally infected pigs [see comments]. J Infect Dis 175:7–15PubMedCrossRefGoogle Scholar
  153. Wesley RD, Mengeling WL, Andreyev V and Lager LM (1996) Differentiation of vaccine (Strain RespPRRS) and field strains of porcine reproductive and respiratory syndrome virus by restriction enzyme analysis. In Proc. 27th Annual Meeting Am Assoc Swine Pract pp141–143Google Scholar
  154. West KH, Bystrom JM and Wjnarowicz C (1999) Myocarditis and abortion associated with intrauterine infection of sows with porcine circovirus 2. Vet Diagn Invest 11:530–532CrossRefGoogle Scholar
  155. Widen BF, Lowings JP, Belak S and Banks M (1999) Development of a PCR system for porcine cytomegalovirus detection and determination of the putative partial sequence of its DNA polymerase gene. Epidemiol Infect 123:177–80PubMedCrossRefGoogle Scholar
  156. Wills RW, Zimmerman JJ, Yoon KJ, Swenson SL, McGinley MJ, Hill HT, Platt KB, Christopher-Hennings J and Nelson EA (1997) Porcine reproductive and respiratory syndrome virus: A persistent infection. Vet Microbiol 55:231–240PubMedCrossRefGoogle Scholar
  157. Wong SC, Ooi MH, Wong MNL, Tio PH, Solomon T and Cardosa MJ (2001) Late presentation of Nipah virus encephalitis and kinetics of the humoral immune response. J Neurol Neurosurg Psychiatry 71:552–554PubMedCrossRefGoogle Scholar
  158. Yoon IJ, Joo HS, Christianson WT, Kim HS, Collins JE, Morrison RB and Dial GD (1992) An indirect fluorescent antibody test for the detection of antibody to swine infertility and respiratory syndrome virus in swine sera. J Vet Diagn Invest 4:144–147PubMedCrossRefGoogle Scholar
  159. Yoon KJ, Wu LL, Zimmerman JJ, Hill HT and Platt KB (1996) Antibody-dependent enhancement (ADE) of porcine reproductive and respiratory syndrome virus (PRRSV) infection in pigs. Viral Immunol 9:51–63PubMedCrossRefGoogle Scholar

Copyright information

© Springer-Verlag Berlin Heidelberg 2003

Authors and Affiliations

  • P. S. Paul
    • 1
  • P. Halbur
    • 2
  • B. Janke
    • 2
  • H. Joo
    • 3
  • P. Nawagitgul
    • 4
  • J. Singh
    • 2
  • S. Sorden
    • 2
  1. 1.Department of Veterinary and Biomedical SciencesUniversity of Nebraska-LincolnLincolnUSA
  2. 2.College of Veterinary MedicineIowa State UniversityAmesUSA
  3. 3.College of Veterinary MedicineUniversity of MinnesotaSaint PaulUSA
  4. 4.Faculty of Veterinary MedicineKasetsart UniversityKasetsartThailand

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