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Reactionary and Reparative Dentin-Like Structures

  • Michel GoldbergEmail author
Chapter

Abstract

Reactionary dentin-like is produced by odontoblasts and Hoehl’s cell after a carious decay, beneath a calciotraumatic line. Five SIBLINGs have been identified, together with the metalloproteinase-2 (MMP-2), whereas SMAD-2 and SMAD-4 are implicated in the synthesis of collagen. Reparative dentin is similar to a bone-like (osteodentin) structure and produced by pulp cells. BMPs, OP-1 or BMP-7, and other growth factors have also been identified in the reparative dentin. A series of SIBLINGs, fibronectin, ameloblastin, MMP-3, and MMP-9 are implicated in the formation of reparative dentin allowing the closure of a pulp exposure.

Keywords

Dental Pulp Pulp Cell Pulp Chamber Dental Pulp Cell Dental Pulp Stem Cell 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

References

  1. 1.
    Ohshima H. Ultrastructural changes in odontoblasts and pulp capillaries following cavity preparation in rat molars. Arch Histol Cytol. 1990;53:423–38.PubMedCrossRefGoogle Scholar
  2. 2.
    Six N, Lasfargues JJ, Goldberg M. Differential repair responses in the coronal and radicular areas of the exposed rat molar pulp induced by recombinant human bone morphogenetic protein 7 (osteogenic protein 1). Arch Oral Biol. 2002;47:177–87.PubMedCrossRefGoogle Scholar
  3. 3.
    Smith AJ, Cassidy N, Perry H, Bégue-Kirn C, Ruch J-V, Lesot H. Reactionary dentinogenesis. Int J Dev Biol. 1995;39:273–80.PubMedGoogle Scholar
  4. 4.
    Rutherford B, Spångberg L, Tucker M, Charette M. Transdentinal stimulation of reparative dentine formation by osteogenic protein-1 in monkeys. Arch Oral Biol. 1995;40:681–3.PubMedCrossRefGoogle Scholar
  5. 5.
    Smith AJ, Tobias RS, Murray PE. Transdentinal stimulation of reactionary dentinogenesis in ferrets by dentine matrix components. J Dent. 2001;29:341–6.PubMedCrossRefGoogle Scholar
  6. 6.
    Smith AJ, Murray PE, Sloan AJ, Matthews JB, Zhao S. Trans-dentinal stimulation of tertiary dentinogenesis. Adv Dent Res. 2001;15:51–4.PubMedCrossRefGoogle Scholar
  7. 7.
    Murray PE, About I, Lumley PJ, Franquin JC, Remusat M, Smith AJ. Cavity remaining dentin thickness and pulpal activity. Am J Dent. 2002;15:41–6.PubMedGoogle Scholar
  8. 8.
    Moses KD, Butler WT, Qin C. Immunohistochemical study of small integrin-binding ligand, N-linked glycoproteins in reactionary dentin of rat molars at different ages. Eur J Oral Sci. 2006;114:216–22.PubMedCrossRefGoogle Scholar
  9. 9.
    Yuan G, Yang G, Song G, Chen Z, Chen S. Immunohistochemical localization of the NH(2)-terminal and COOH-terminal fragments of dentin sialoprotein in mouse teeth. Cell Tissue Res. 2012;349:605–14.PubMedCentralPubMedCrossRefGoogle Scholar
  10. 10.
    Aguiar MC, Arana-Chavez VE. Ultrastructural and immunocytochemical analyses of osteopontin in reactionary and reparative dentine formed after extrusion of upper rat incisors. J Anat. 2007;210:418–27.PubMedCrossRefGoogle Scholar
  11. 11.
    Charadram N, Farahani RM, Harty D, Rathsam C, Swain MV, Hunter N. Regulation of reactionary dentin formation by odontoblasts in response to polymicrobial invasion of dentin matrix. Bone. 2012;50:265–75.PubMedCentralPubMedCrossRefGoogle Scholar
  12. 12.
    Charadram N, Austin C, Trimby P, Simonian M, Swain MV, Hunter N. Structural analysis of reactionary dentin formed in response to polymicrobial invasion. J Struct Biol. 2013;181:207–22.PubMedCentralPubMedCrossRefGoogle Scholar
  13. 13.
    Yang IS, Lee DS, Park JT, Kim HJ, Son HH, Park JC. Tertiary dentin formation after direct pulp capping with odontogenic ameloblast-associated protein in rat teeth. J Endod. 2010;36:1956–62.PubMedCrossRefGoogle Scholar
  14. 14.
    D’Souza RN, Bachman T, Baumgardner KR, Butler WT, Litz M. Characterization of cellular responses involved in reparative dentinogenesis in rat molars. J Dent Res. 1995;74:702–9.PubMedCrossRefGoogle Scholar
  15. 15.
    Kim JH, Lee EH, Park HJ, Park EK, Kwon TG, Shin HI, Cho JY. The role of lysyl oxidase-like 2 in the odontogenic differentiation of human dental pulp stem cells. Mol Cells. 2013;35:543–9.PubMedCentralPubMedCrossRefGoogle Scholar
  16. 16.
    Stanislawski L, Carreau JP, Pouchelet M, Chen ZH, Goldberg M. In vitro culture of human dental pulp cells: some aspects of cells emerging early from the explant. Clin Oral Investig. 1997;1:131–40.PubMedCrossRefGoogle Scholar
  17. 17.
    Sela J, Tamari I, Hirschfeld Z, Bab I. Transmission electron microscopy of reparative dentin in rat molar pulps. Primary mineralization via extracellular matrix vesicles. Acta Anat (Basel). 1981;109:247–51.CrossRefGoogle Scholar
  18. 18.
    Frozoni M, Balic A, Sagomonyants K, Zaia AA, Line SR, Mina M. A feasibility study for the analysis of reparative dentinogenesis in pOBCol3.6GFPtpz transgenic mice. Int Endod J. 2012;45:907–14.PubMedCentralPubMedCrossRefGoogle Scholar
  19. 19.
    Zheng Y, Wang XY, Wang YM, Liu XY, Zhang CM, Hou BX, Wang SL. Dentin regeneration using deciduous pulp stem/progenitor cells. J Dent Res. 2012;91:676–82.PubMedCrossRefGoogle Scholar
  20. 20.
    Scheller EL, Chang J, Wang CY. Wnt/β-catenin inhibits dental pulp stem cell differentiation. J Dent Res. 2008;87:126–30.PubMedCentralPubMedCrossRefGoogle Scholar
  21. 21.
    Sloan AJ, Smith AJ. Stimulation of the dentine-pulp complex of rat incisor teeth by transforming growth factor-beta isoforms 1–3 in vitro. Arch Oral Biol. 1999;44:149–56.PubMedCrossRefGoogle Scholar
  22. 22.
    Hwang YC, Hwang IN, Oh WM, Park JC, Lee DS, Son HH. Influence of TGF-beta1 on the expression of BSP, DSP, TGF-beta1 receptor I and Smad proteins during reparative dentinogenesis. J Mol Histol. 2008;39:153–60.PubMedCrossRefGoogle Scholar
  23. 23.
    Kitamura C, Kimura K, Nakayama T, Terashita M. Temporal and spatial expression of c-jun and jun-B proto-oncogenes in pulp cells involved with reparative dentinogenesis after cavity preparation of rat molars. J Dent Res. 1999;78:673–80.PubMedCrossRefGoogle Scholar
  24. 24.
    Rutherford RB, Wahle J, Tucker M, Rueger D, Charette M. Induction of reparative dentine formation in monkeys by recombinant human osteogenic protein-1. Arch Oral Biol. 1993;38:571–6.PubMedCrossRefGoogle Scholar
  25. 25.
    Rutherford RB, Spångberg L, Tucker M, Rueger D, Charette M. The time-course of the induction of reparative dentine formation in monkeys by recombinant human osteogenic protein-1. Arch Oral Biol. 1994;39:833–8.PubMedCrossRefGoogle Scholar
  26. 26.
    Rutherford RB, Gu K. Treatment of inflamed ferret dental pulps with recombinant bone morphogenetic protein-7. Eur J Oral Sci. 2000;108:202–6.PubMedCrossRefGoogle Scholar
  27. 27.
    Rutherford RB. BMP-7 gene transfer to inflamed ferret dental pulps. Eur J Oral Sci. 2001;109:422–4.PubMedCrossRefGoogle Scholar
  28. 28.
    Iohara K, Nakashima M, Ito M, Ishikawa M, Nakasima A, Akamine A. Dentin regeneration by dental pulp stem cell therapy with recombinant human bone morphogenetic protein 2. J Dent Res. 2004;83:590–5.PubMedCrossRefGoogle Scholar
  29. 29.
    Gu K, Smoke RH, Rutherford RB. Expression of genes for bone morphogenetic proteins and receptors in human dental pulp. Arch Oral Biol. 1996;41:919–23.PubMedCrossRefGoogle Scholar
  30. 30.
    Nakatomi M, Ida-Yonemochi H, Ohshima H. Lymphoid enhancer-binding factor 1 expression precedes dentin sialophosphoprotein expression during rat odontoblast differentiation and regeneration. J Endod. 2013;39:612–8.PubMedCrossRefGoogle Scholar
  31. 31.
    Nakashima M, Mizunuma K, Murakami T, Akmine A. Induction of dental pulp stem cell differentiation into odontoblasts by electroporation-mediated gene delivery of growth/differentiation factor 11(Gdf11). Gene Ther. 2002;9:814–8.PubMedCrossRefGoogle Scholar
  32. 32.
    Paula-Silva FWG, Ghosh A, Silva LAB, Kapila YL. TNF-α promotes an odontoblastic phenotype in dental pulp cells. J Dent Res. 2009;88:339–44.PubMedCentralPubMedCrossRefGoogle Scholar
  33. 33.
    Muromachi K, Kamio N, Matsumoto T, Matsushima K. Role of CTGF/CCN2 in reparative dentinogenesis in human dental pulp. J Oral Sci. 2012;54:47–54.PubMedCrossRefGoogle Scholar
  34. 34.
    Lovschall H, Fejerskov O, Flyvbjerg A. Pulp-capping with recombinant human insulin-like growth factor I (rhIGF-I) in rat molars. Adv Dent Res. 2001;15:108–12.PubMedCrossRefGoogle Scholar
  35. 35.
    Murakami Masuda Y, Wang X, Yokose S, Yamada Y, Kimura Y, Okano T, Matsumoto K. Effect of glypican-1 gene on the pulp cells during the reparative dentine process. Cell Biol Int. 2010;34:1069–74.PubMedCrossRefGoogle Scholar
  36. 36.
    Chen L, Liu H, Sun W, Bai X, Karaplis AC, Goltzman D, Miao D. Fibroblast growth factor 23 overexpression impacts negatively on dentin mineralization and dentinogenesis in mice. Clin Exp Pharmacol Physiol. 2011;38:395–402.PubMedCrossRefGoogle Scholar
  37. 37.
    Nagata K, Huang YH, Ohsaki Y, Kukita T, Nakata M, Kurisu K. Demonstration of type III collagen in the dentin of mice. Matrix. 1992;12:448–55.PubMedCrossRefGoogle Scholar
  38. 38.
    Yasuda Y, Izumikawa M, Okamoto K, Tsukuba T, Saito T. Dentin phosphophoryn promotes cellular migration of human dental pulp cells. J Endod. 2008;34:575–8.PubMedCrossRefGoogle Scholar
  39. 39.
    Decup F, Six N, Palmier B, Buch D, Lasfargues JJ, Salih E, Goldberg M. Bone sialoprotein-induced reparative dentinogenesis in the pulp of rat’s molar. Clin Oral Investig. 2000;4:110–9.PubMedCrossRefGoogle Scholar
  40. 40.
    Goldberg M, Six N, Decup F, Buch D, Soheili Majd E, Lasfargues JJ, Salih E, Stanislawski L. Application of bioactive molecules in pulp-capping situations. Adv Dent Res. 2001;15:91–5.PubMedCrossRefGoogle Scholar
  41. 41.
    Almushayt A, Narayanan K, Zaki AE, George A. Dentin matrix protein 1 induces cyto differentiation of dental pulp stem cells into odontoblasts. Gene Ther. 2006;13:611–20.PubMedCrossRefGoogle Scholar
  42. 42.
    Six N, Septier D, Chaussain-Miller C, Blacher R, DenBesten P, Goldberg M. Dentonin, a MEPE fragment, initiates pulp-healing response to injury. J Dent Res. 2007;86:780–5.PubMedCrossRefGoogle Scholar
  43. 43.
    Yoshiba K, Yoshiba N, Nakamura H, Iwaku M, Ozawa H. Immunolocalization of fibronectin during reparative dentinogenesis in human teeth after pulp capping with calcium hydroxide. J Dent Res. 1996;75:1590–7.PubMedCrossRefGoogle Scholar
  44. 44.
    Nakamura Y, Slaby I, Spahr A, Pezeshki G, Matsumoto K, Lyngstadaas SP. Ameloblastin fusion protein enhances pulpal healing and dentin formation in porcine teeth. Calcif Tissue Int. 2006;78:278–84.PubMedCrossRefGoogle Scholar
  45. 45.
    Lacerda-Pinheiro S, Septier D, Tompkins K, Veis A, Goldberg M, Chardin H. Amelogenin gene splice products A+4 and A-4 implanted in soft tissue determine the reorientation of CD45-positive cells to an osteo- chondrogenic lineage. J Biomed Mater Res A. 2006;79:1015–22.PubMedCrossRefGoogle Scholar
  46. 46.
    Huang Y, Goldberg M, Le T, Qiang R, Warner D, Witkowska HE, Liu H, Zhu L, DenBesten P, Li W. Amelogenin exons 8 and 9 encoded peptide enhances Leucine Rich Amelogenin Peptide (LRAP) mediated dental pulp repair. Cells Tissues Organs. 2012;196:151–60.PubMedCentralPubMedCrossRefGoogle Scholar
  47. 47.
    Zheng L, Amano K, Iohara K, Ito M, Imabayashi K, Into T, Matsushita K, Nakamura H, Nakashima M. Matrix metalloproteinase-3 accelerates wound healing following dental pulp injury. Am J Pathol. 2009;175:1905–14.PubMedCentralPubMedCrossRefGoogle Scholar

Copyright information

© Springer-Verlag Berlin Heidelberg 2014

Authors and Affiliations

  1. 1.Department of Oral Biology, Institut National de la Santé et de la Recherche MédicaleUniversité Paris DescartesParisFrance

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