Characterization of Ribonucleic Acids and Their Modifications by Fourier Transform Ion Cyclotron Resonance Mass Spectrometry

Chapter
Part of the Physical Chemistry in Action book series (PCIA)

Abstract

Recent advances in electrospray ionization and the extension of radical ion based dissociation techniques to ribonucleic acids (RNA) were key factors for developing top-down mass spectrometry as a powerful method for the detailed characterization of posttranscriptional and synthetic modifications of RNA. This new approach identifies and localizes all mass-altering modifications without the need for labeling reactions, and can be used for characterization of RNA of unknown sequence.

Keywords

RNA Posttranscriptional modifications Synthetic modifications Collisionally activated dissociation Electron detachment dissociation Top-down mass spectrometry Fourier transform ion cyclotron resonance Mass spectrometry 

Abbreviations/Formulae

BIRD

Blackbody infrared multiphoton dissociation

CAD

Collisionally activated dissociation (synonymous with CID)

CID

Collision-induced dissociation (synonymous with CAD)

cDNA

Complementary desoxyribonucleic acid

DNA

Desoxyribonucleic acid

ECD

Electron capture dissociation

EDD

Electron detachment dissociation

EPD

Electron photodetachment dissociation

ESI

Electrospray ionization

FTICR

Fourier transform ion cyclotron resonance

IRMPD

Infrared multiphoton dissociation

MALDI

Matrix-assisted laser desorption/ionization

MS

Mass spectrometry

nETD

Negative electron transfer dissociation

nt

Nucleotide(s)

PTM

Posttranscriptional modification

RNA

Ribonucleic acid

siRNA

Small interfering RNA

tRNA

Transfer RNA

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Copyright information

© Springer-Verlag Berlin Heidelberg 2014

Authors and Affiliations

  1. 1.Institute of Organic Chemistry and Center for Molecular Biosciences Innsbruck (CMBI)University of InnsbruckInnsbruckAustria

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