Fluorescence Techniques in Cell Biology pp 219-233 | Cite as
Rapid Microfluorometry for Biochemistry of the Living Cell in Correlation with Cytomorphology and Transport Phenomena
Abstract
The living cell represents the ultimate biochemical system, the complexity of which is based on a structural microarchitecture forming compartments for a great variety of reacting substances (Chance 1963). For the purpose of description, more or less complicated “cell models” can be constructed (Zeigler and Weinberg 1970). Such models, however, say little about the dynamic state of the living cell, in which microcompartments containing pools of various chemicals form and disappear continuously. To probe this multireaction system, one obviously needs methods which can qualitatively and quantitatively analyze amounts and states of substances within very small and localized portions of the functioning cell.
Keywords
Substrate Utilization Pyridine Nucleotide Ascites Cell Glycolytic Intermediate Chang Liver CellPreview
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References
- ATKINSON, D.E., (1965), Science, 150, 851.PubMedCrossRefGoogle Scholar
- CHANCE, B., (1963), Ann. N.Y. Acad. Sci., 108,322.PubMedCrossRefGoogle Scholar
- CHANCE, B. and Baltscheffsky, H., (1958), J. Biol. Chem., 233, 736.PubMedGoogle Scholar
- CHANCE, B., Kohen, E., Kohen, C. and Legallais, V., (1967), In: Advances in Enzyme Regulation, (G. Weber, ed.), Vol. 5, Pergamon Press, New York, 3.Google Scholar
- CHANCE, B. and Thorell, B., (1959), J. Biol. Chem., 234, 3044.PubMedGoogle Scholar
- DE DUVE, C., (1973), In: Wenner-Gren Symposium on Structure and Function of Oxidation-Reduction Enzymes, Wenner-Gren Center, Stockholm, August 23–27, 1970 (A. Åeson and A. Ehrenberg, eds.), Pergamon Press Ltd., Oxford. farber, E., (1963), Adv. Cancer Res., 7, 383.Google Scholar
- HESS, B., (1971), In: Photo Synthesis, Bio energetics, Regulation, Origin of Life, Proceedings First European Biophysics Congress, (E. Broda, A. Locker, and H. Springer-Lederer, eds.), Vol. 4, Verlag der Wiener Medizinischen Academie, Vienna, 447.Google Scholar
- JOHANNISSON, E. and Hagenfeldt, K. (1971), Karolinska Symposia on Research Methods in Reproductive Endocrinology, Third Symposium: In: In-Vitro Metabolism in Reproductive Cell Biology, Geneva, January 25–27, (A. Diczfalusy, ed.), Forum, Copenhagen, 81.Google Scholar
- KALANT, N. and Beitner, R., (1971), J. Biol. Chem., 246, 504.PubMedGoogle Scholar
- KALCKAR, H.M., (1953), Biochim. Biophys. Acta, 12, 250.PubMedCrossRefGoogle Scholar
- KENNEY, F.T., (1970), In: Mammalian Protein Metabolism, (H.N. Munro, ed.), Academic Press, New York, 131.Google Scholar
- KEYHANI, E. and Chance, B., (1971), FEBS Letters, 17, 127.PubMedCrossRefGoogle Scholar
- KLINGENBERG, M. and Pfaff, E., (1966), In: Methods in Enzymology, (J.M. Tager, S. Papa, E. Quagliariello, and E.C. Slater, eds.), Vol. 10 Elsevier, Amsterdam, 180.Google Scholar
- KOHEN, E., Kohen, C. and Jenkins, W., (1966), Exptl. Cell Res., 44, 175.PubMedCrossRefGoogle Scholar
- KOHEN, E., Kohen, C. and Thorell, B., (1968a), Acta Pharm. Toxicol., 26, 556.CrossRefGoogle Scholar
- KOHEN, E., Kohen, C. and Thorell, B., (1968b), Exptl. Cell Res., 49, 169.PubMedCrossRefGoogle Scholar
- KOHEN, E., Kohen, C. and Thorell, B., (1970a), Biochim. Biophys Acta, 198, 1.PubMedGoogle Scholar
- KOHEN, E., Kohen, C. and Thorell, B., (1970b), Exptl. Cell Res. 59, 307.PubMedCrossRefGoogle Scholar
- KOHEN, E., Kohen, C. and Thorell, B., (1970c), Mikrochimica Acta, 1190.Google Scholar
- KOHEN, e., Kohen, C. and Thorell, B., (1970d), Symposium of Homologies in Enzymes and Metabolic Pathways and Symposium of Metabolic Alterations in Cancer, (W.J. whelan and J. Schultz, eds.), North Holland Press, Amsterdam, 481.Google Scholar
- KOHEN, E., Kohen, C. and Thorell, B.,(1970e), In: Wenner-Green Symposium on Structure and Function of Oxidation-Reduction Enzymes, Wenner-Gren Center, Stockholm, August 23–27, (Å. Åkeson and A. Ehrenberg eds.) Pergamon Press, Ltd., Oxford, 557.Google Scholar
- KOHEN, E., Kohen, C. and Thorell, B., (1971a), Biochim. Biophys. Acta, 234, 531.PubMedCrossRefGoogle Scholar
- KOHEN, E., Kohen, C. and Thorell, B., (1971b), Exptl. Cell Res. 64, 339.PubMedCrossRefGoogle Scholar
- KOHEN, E., Kohen, C. and Thorell, B., (1971c), Hoppe-Seyler’s Z. Physiol. Chemie., 352, 635.CrossRefGoogle Scholar
- KOHEN, E., Kohen, C. and Thorell, B., (1971d) In: Photosynthesis, Bioenergetics Regulation, Origin of Life. Proceedings First European Biophysics Congress, (E. Broda, A. Locker and H. Springer-Lederer, eds.) Vol. 4, Verlag der Wiener Medizinischen Akademie, Vienna, 465.Google Scholar
- KOHEN, E., Kohen, C. and Thorell, B.,(1972), Mikrochimica Acta, 103.Google Scholar
- KOHEN, E., Kohen, C., Thorell, B. and Wagener, G., (1973), This Volume.Google Scholar
- KOHEN, E., Siebert, G. and Kohen, C., (1972), Hoppe Seylers r s Z. Physiol. Chemie, 352, 927.CrossRefGoogle Scholar
- KREBS, H.A., (1968) In: Advances in Enzyme Regulation, (G. Weber ed.), Vol. 6, Pergamon Press, Oxford, 467.Google Scholar
- KREBS, H.A. and Veech, R.L., (1969), In: Advances in Enzyme Regulation, (G. Weber ed.) Vol. 7, Pergamon Press, New York, 397.Google Scholar
- LARDY, H., (1965), In: Control of Energy Metabolism, (B. Chance, R. Estabrook and J.R. Williamson, eds.), Academic Press, New York, 69.Google Scholar
- LELOIR, L.F., (1951, In: Phosphorus Metabolism, Vol. 1, Johns Hopkins Press, Baltimore, 67.Google Scholar
- LUPU, C.J. and Farber, E., (1954), Proc. Soc. Exp/Biol., 86, 701.Google Scholar
- MANTEIFEL, V.M. and Meisel, M.N., (1966), Fed. Proc, Translation Suppl., 25, T 981–88.Google Scholar
- MEYERHOF, O. and Wilson, J.R., (1949), Arch. Biochem., 21, 22.PubMedGoogle Scholar
- ORRENIUS, S., (1965), In: Studies on the Drug-hydroxylating Enzyme of Rat Liver Microsomes, Almquist and Wiksells Boktryckeri AB, Uppsala.Google Scholar
- STROMINGER, J.C., Maxwell, E.S., Axelrod, J., and Kalckar, H.M., (1957), J. Biol. Chem. 224, 79.PubMedGoogle Scholar
- SUND, H., (1968), In: Biological Oxidations, (T. P. Singer, ed.) Interscience Publishers, New York, 603.Google Scholar
- SUZUKI, K. (1969), Science, 163, 81.PubMedCrossRefGoogle Scholar
- VELICK, S.F., (1958), J. Biol. Chem., 233, 1455.Google Scholar
- ZEIGLER, B.P., and Weinberg, R., (1970), J. Theor. Biol., 29, 35.PubMedCrossRefGoogle Scholar