Rapid Microfluorometry for Biochemistry of the Living Cell in Correlation with Cytomorphology and Transport Phenomena

  • Elli Kohen
  • Bo Thorell
  • Cahide Kohen
  • Moritz Michaelis

Abstract

The living cell represents the ultimate biochemical system, the complexity of which is based on a structural microarchitecture forming compartments for a great variety of reacting substances (Chance 1963). For the purpose of description, more or less complicated “cell models” can be constructed (Zeigler and Weinberg 1970). Such models, however, say little about the dynamic state of the living cell, in which microcompartments containing pools of various chemicals form and disappear continuously. To probe this multireaction system, one obviously needs methods which can qualitatively and quantitatively analyze amounts and states of substances within very small and localized portions of the functioning cell.

Keywords

Substrate Utilization Pyridine Nucleotide Ascites Cell Glycolytic Intermediate Chang Liver Cell 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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References

  1. ATKINSON, D.E., (1965), Science, 150, 851.PubMedCrossRefGoogle Scholar
  2. CHANCE, B., (1963), Ann. N.Y. Acad. Sci., 108,322.PubMedCrossRefGoogle Scholar
  3. CHANCE, B. and Baltscheffsky, H., (1958), J. Biol. Chem., 233, 736.PubMedGoogle Scholar
  4. CHANCE, B., Kohen, E., Kohen, C. and Legallais, V., (1967), In: Advances in Enzyme Regulation, (G. Weber, ed.), Vol. 5, Pergamon Press, New York, 3.Google Scholar
  5. CHANCE, B. and Thorell, B., (1959), J. Biol. Chem., 234, 3044.PubMedGoogle Scholar
  6. DE DUVE, C., (1973), In: Wenner-Gren Symposium on Structure and Function of Oxidation-Reduction Enzymes, Wenner-Gren Center, Stockholm, August 23–27, 1970 (A. Åeson and A. Ehrenberg, eds.), Pergamon Press Ltd., Oxford. farber, E., (1963), Adv. Cancer Res., 7, 383.Google Scholar
  7. HESS, B., (1971), In: Photo Synthesis, Bio energetics, Regulation, Origin of Life, Proceedings First European Biophysics Congress, (E. Broda, A. Locker, and H. Springer-Lederer, eds.), Vol. 4, Verlag der Wiener Medizinischen Academie, Vienna, 447.Google Scholar
  8. JOHANNISSON, E. and Hagenfeldt, K. (1971), Karolinska Symposia on Research Methods in Reproductive Endocrinology, Third Symposium: In: In-Vitro Metabolism in Reproductive Cell Biology, Geneva, January 25–27, (A. Diczfalusy, ed.), Forum, Copenhagen, 81.Google Scholar
  9. KALANT, N. and Beitner, R., (1971), J. Biol. Chem., 246, 504.PubMedGoogle Scholar
  10. KALCKAR, H.M., (1953), Biochim. Biophys. Acta, 12, 250.PubMedCrossRefGoogle Scholar
  11. KENNEY, F.T., (1970), In: Mammalian Protein Metabolism, (H.N. Munro, ed.), Academic Press, New York, 131.Google Scholar
  12. KEYHANI, E. and Chance, B., (1971), FEBS Letters, 17, 127.PubMedCrossRefGoogle Scholar
  13. KLINGENBERG, M. and Pfaff, E., (1966), In: Methods in Enzymology, (J.M. Tager, S. Papa, E. Quagliariello, and E.C. Slater, eds.), Vol. 10 Elsevier, Amsterdam, 180.Google Scholar
  14. KOHEN, E., Kohen, C. and Jenkins, W., (1966), Exptl. Cell Res., 44, 175.PubMedCrossRefGoogle Scholar
  15. KOHEN, E., Kohen, C. and Thorell, B., (1968a), Acta Pharm. Toxicol., 26, 556.CrossRefGoogle Scholar
  16. KOHEN, E., Kohen, C. and Thorell, B., (1968b), Exptl. Cell Res., 49, 169.PubMedCrossRefGoogle Scholar
  17. KOHEN, E., Kohen, C. and Thorell, B., (1970a), Biochim. Biophys Acta, 198, 1.PubMedGoogle Scholar
  18. KOHEN, E., Kohen, C. and Thorell, B., (1970b), Exptl. Cell Res. 59, 307.PubMedCrossRefGoogle Scholar
  19. KOHEN, E., Kohen, C. and Thorell, B., (1970c), Mikrochimica Acta, 1190.Google Scholar
  20. KOHEN, e., Kohen, C. and Thorell, B., (1970d), Symposium of Homologies in Enzymes and Metabolic Pathways and Symposium of Metabolic Alterations in Cancer, (W.J. whelan and J. Schultz, eds.), North Holland Press, Amsterdam, 481.Google Scholar
  21. KOHEN, E., Kohen, C. and Thorell, B.,(1970e), In: Wenner-Green Symposium on Structure and Function of Oxidation-Reduction Enzymes, Wenner-Gren Center, Stockholm, August 23–27, (Å. Åkeson and A. Ehrenberg eds.) Pergamon Press, Ltd., Oxford, 557.Google Scholar
  22. KOHEN, E., Kohen, C. and Thorell, B., (1971a), Biochim. Biophys. Acta, 234, 531.PubMedCrossRefGoogle Scholar
  23. KOHEN, E., Kohen, C. and Thorell, B., (1971b), Exptl. Cell Res. 64, 339.PubMedCrossRefGoogle Scholar
  24. KOHEN, E., Kohen, C. and Thorell, B., (1971c), Hoppe-Seyler’s Z. Physiol. Chemie., 352, 635.CrossRefGoogle Scholar
  25. KOHEN, E., Kohen, C. and Thorell, B., (1971d) In: Photosynthesis, Bioenergetics Regulation, Origin of Life. Proceedings First European Biophysics Congress, (E. Broda, A. Locker and H. Springer-Lederer, eds.) Vol. 4, Verlag der Wiener Medizinischen Akademie, Vienna, 465.Google Scholar
  26. KOHEN, E., Kohen, C. and Thorell, B.,(1972), Mikrochimica Acta, 103.Google Scholar
  27. KOHEN, E., Kohen, C., Thorell, B. and Wagener, G., (1973), This Volume.Google Scholar
  28. KOHEN, E., Siebert, G. and Kohen, C., (1972), Hoppe Seylers r s Z. Physiol. Chemie, 352, 927.CrossRefGoogle Scholar
  29. KREBS, H.A., (1968) In: Advances in Enzyme Regulation, (G. Weber ed.), Vol. 6, Pergamon Press, Oxford, 467.Google Scholar
  30. KREBS, H.A. and Veech, R.L., (1969), In: Advances in Enzyme Regulation, (G. Weber ed.) Vol. 7, Pergamon Press, New York, 397.Google Scholar
  31. LARDY, H., (1965), In: Control of Energy Metabolism, (B. Chance, R. Estabrook and J.R. Williamson, eds.), Academic Press, New York, 69.Google Scholar
  32. LELOIR, L.F., (1951, In: Phosphorus Metabolism, Vol. 1, Johns Hopkins Press, Baltimore, 67.Google Scholar
  33. LUPU, C.J. and Farber, E., (1954), Proc. Soc. Exp/Biol., 86, 701.Google Scholar
  34. MANTEIFEL, V.M. and Meisel, M.N., (1966), Fed. Proc, Translation Suppl., 25, T 981–88.Google Scholar
  35. MEYERHOF, O. and Wilson, J.R., (1949), Arch. Biochem., 21, 22.PubMedGoogle Scholar
  36. ORRENIUS, S., (1965), In: Studies on the Drug-hydroxylating Enzyme of Rat Liver Microsomes, Almquist and Wiksells Boktryckeri AB, Uppsala.Google Scholar
  37. STROMINGER, J.C., Maxwell, E.S., Axelrod, J., and Kalckar, H.M., (1957), J. Biol. Chem. 224, 79.PubMedGoogle Scholar
  38. SUND, H., (1968), In: Biological Oxidations, (T. P. Singer, ed.) Interscience Publishers, New York, 603.Google Scholar
  39. SUZUKI, K. (1969), Science, 163, 81.PubMedCrossRefGoogle Scholar
  40. VELICK, S.F., (1958), J. Biol. Chem., 233, 1455.Google Scholar
  41. ZEIGLER, B.P., and Weinberg, R., (1970), J. Theor. Biol., 29, 35.PubMedCrossRefGoogle Scholar

Copyright information

© Springer-Verlag Berlin · Heidelberg 1973

Authors and Affiliations

  • Elli Kohen
    • 1
  • Bo Thorell
    • 2
  • Cahide Kohen
    • 3
  • Moritz Michaelis
    • 4
  1. 1.Papanicolaou Cancer Research InstituteMiamiUSA
  2. 2.Department of PathologyKarolinska InstituteStockholmSweden
  3. 3.Clinical Faculty, Department of Pathology, School of MedicineUniversity of MiamiMiamiUSA
  4. 4.Ophtalmology Research LaboratorySchool of MedicineUniversity of MarylandBaltimoreUSA

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