Fluorescence Techniques in Cell Biology pp 89-93 | Cite as
Quantitative Determination of DNA and Protein in Single Cells
Chapter
Abstract
Uneven staining of slides and poor reproducibility can make quantitative work difficult or even impossible. These conditions often occur with protein stainings in smears, especially in the case of the histone reactions using fast green or sulfaflavine. Below is a brief discussion of experiments on human leukocytes stained with sulfaflavine at pH 8.0.
Keywords
Fast Green Bromphenol Blue Ethanol Acetic Acid Unfixed Cell Uneven Staining
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© Springer-Verlag Berlin · Heidelberg 1973