Cytokines in Hemopoiesis, Oncology, and AIDS II pp 443-448 | Cite as
Sequential Release of Tumor Necrosis Factor Alpha and Tumor Necrosis Factor Receptors in Complications of Human Bone Marrow Transplantation
Abstract
Previous studies performed in our laboratory demonstrated a high prognostic value of tumor necrosis factor alpha (TNFα) serum levels for outcome and development of transplant-related complications in allogeneic bone marrow transplantation (BMT) [1]. In these studies we combined monitoring of TNFα serum levels by enzyme-linked immunosorbent assay (ELISA) with titration of recombinant human TNFα (rhuTNFα) in individual samples to evaluate the role of serum factors interfering with immunological detection of TNFα. Using this method, we frequently observed induction of TNFα-masking activity which was most pronounced shortly after endogenous release of TNFα. As TNF inhibitors have been found in serum and urine after endogenous release of TNFα and TNF inhibitors have been recently reported to be identical with soluble TNF receptors (sTNFr; [2, 3]), we performed a study comparing release of both TNFα and sTNFr in 26 consecutive patients receiving BMT in our unit.
Keywords
Tumor Necrosis Factor Inhibitor Abstract Book Related Cytokine Soluble Tumor Necrosis Factor Receptor Sequential ReleasePreview
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References
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