Genospecies-Specific Melting Temperature of the recA PCR Product for the Detection of Borrelia burgdorferi sensu lato and Differentiation of Borrelia garinii from Borrelia afzelii and Borrelia burgdorferi sensu stricto
Chapter
Abstract
Lyme borreliosis, manifesting as arthritis, was first reported in the USA in 1977 [1] and the expanded clinical spectrum of this tick-borne multisystem Borrelia infection has since been recognized in many countries. The initial symptom and hallmark of lyme borreliosis is an expanding skin rash called erythema migrans emerging around the tick-bite site. Borrelia burgdorferi sensu stricto, B. garinii, and B. afzelii are the genospecies responsible for human Lyme borreliosis [2]. In Europe B. garinii and B. afzelii are the most prevalent genospecies, whereas B. burgdorferi sensu stricto is the only genospecies encountered in North America [2].
Keywords
Lyme Disease Sensu Stricto Borrelia Burgdorferi Lyme Borreliosis Flagellin Gene
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.
Preview
Unable to display preview. Download preview PDF.
References
- 1.Steere AC, Malawista SE, Snydman DR, et al. (1977) Lyme arthritis: an epidemic of oligoarticular arthritis in children and adults in three Connecticut communities. Arthritis Rheum 20: 7–17PubMedCrossRefGoogle Scholar
- 2.Wang G, van Dam AP, Schwartz I, Dankert J (1999)Molecular typing of Borrelia burgdorferi sensu lato: taxonomic, epidemiological, and clinical implications. Clin Microbiol Rev 12: 633–653Google Scholar
- 3.Marconi RT, Garon CF (1992) Development of polymerase chain reaction primer sets for diagnosis of Lyme disease and for species-specific identification of Lyme disease isolates by 16 S rRNA signature nucleotide analysis (published erratum appears in J Clin Microbiol 31:1026). J Clin Microbiol 30: 2830–2834PubMedGoogle Scholar
- 4.Liebisch G, Sohns B, Bautsch W (1998) Detection and typing of Borrelia burgdorferi sensu lato in Ixodes ricinus ticks attached to human skin by PCR. J Clin Microbiol 36: 3355–3358PubMedGoogle Scholar
- 5.Welsh J, Pretzman C, Postic D, Saint Girons I, Baranton G, McClelland M (1992) Genomic fingerprinting by arbitrarily primed polymerase chain reaction resolves Borrelia burgdorferi into three distinct phyletic groups. Int J Syst Bacteriol 42: 370–377PubMedCrossRefGoogle Scholar
- 6.Valsangiacomo C, Balmelli T, Piffaretti JC (1997) A phylogenetic analysis of Borrelia burgdorferi sensu lato based on sequence information from the hbb gene, coding for a histone-like protein. Int J Syst Bacteriol 47: 1–10PubMedCrossRefGoogle Scholar
- 7.Fukunaga M, Hamase A (1995) Outer surface protein C gene sequence analysis of Borrelia burgdorferi sensu lato isolates from Japan. J Clin Microbiol 33: 2415–2420PubMedGoogle Scholar
- 8.Fukunaga M, Koreki Y (1996) A phylogenetic analysis of Borrelia burgdorferi sensu lato isolates associated with Lyme disease in Japan by flagellin gene sequence determination. Int J Syst Bacteriol 46: 416–421PubMedCrossRefGoogle Scholar
- 9.Gassmann GS, Jacobs E, Deutzmann R, Gobel UB (1991) Analysis of the Borrelia burgdorferi GeHo fla gene and antigenic characterization of its gene product. J Bacterial 173: 1452–1459Google Scholar
- 10.Misonne MC, Hoet PP (1998) Species-specific plasmid sequences for PCR identification of the three species of Borrelia burgdorferi sensu lato involved in Lyme disease. J Clin Microbiol 36: 269–272PubMedGoogle Scholar
- 11.Liveris D, Wormser GP, Nowakowski J et al. (1996) Molecular typing of Borrelia burgdorferi from Lyme disease patients by PCR-restriction fragment length polymorphism analysis. J Clin Microbiol 34: 1306–1309PubMedGoogle Scholar
- 12.Postic D, Assous MV, Grimont PA, Baranton G (1994) Diversity of Borrelia burgdorferi sensu lato evidenced by restriction fragment length polymorphism of rrf (5 S)-rrl (23 S) intergenic spacer amplicons. Int J Syst Bacteriol 44: 743–752PubMedCrossRefGoogle Scholar
- 13.Rijpkema SG, Herbes RG, Verbeek-De Kruif N, Schellekens JF (1996) Detection of four species of Borrelia burgdorferi sensu lato in Ixodes ricinus ticks collected from roe deer (Capreolus capreolus) in The Netherlands. Epidemiol Infect 117: 563–566PubMedCrossRefGoogle Scholar
- 14.Fraser CM, Casjens S, Huang WM et al. (1997) Genomic sequence of a Lyme disease spirochete, Borrelia burgdorferi. Nature 390: 580–586PubMedCrossRefGoogle Scholar
- 15.Morrison TB, Ma Y, Weis JH, Weis JJ (1999) Rapid and sensitive quantification of Borrelia burgdorferi-infected mouse tissues by continuous fluorescent monitoring of PCR. J Clin Microbiol 37: 987–992PubMedGoogle Scholar
- 16.Schmidt B, Muellegger RR, Stockenhuber C et al. (1996) Detection of Borrelia burgdorferispecific DNA in urine specimens from patients with erythema migrans before and after antibiotic therapy. J Clin Microbiol 34: 1359–1363PubMedGoogle Scholar
- 17.Junttila J, Peltomaa M, Soini H, Marjamaki M, Viljanen MK (1999) Prevalence of Borrelia burgdorferi in Ixodes ricinus ticks in urban recreational areas of Helsinki. J Clin Microbiol 37: 1361–1365PubMedGoogle Scholar
- 18.SantaLucia J Jr (1998) A unified view of polymer, dumbbell, and oligonucleotide DNA nearest-neighbor thermodynamics. Proc Natl Acad Sci USA 95: 1460–1465PubMedCrossRefGoogle Scholar
- 19.Ririe KM, Rasmussen RP, Wittwer CT (1997) Product differentiation by analysis of DNA melting curves during the polymerase chain reaction. Anal Biochem 245: 154–160PubMedCrossRefGoogle Scholar
Copyright information
© Springer-Verlag Berlin Heidelberg 2002