Flow Cytometry in Plant Research: A Success Story

  • Jan Vrána
  • Petr Cápal
  • Martina Bednářová
  • Jaroslav Doležel
Part of the Plant Cell Monographs book series (CELLMONO, volume 22)


Flow cytometry is a powerful technique with numerous applications in biomedical research, including immunology, haematology, oncology and other fields. It has also found important applications in plant science where it accompanied scientists for almost four decades. Without its invaluable outcomes, some areas of plant research would not be in the position where they are now. This chapter focuses on exploitation of this state-of-the-art technology for studying plants at cellular and subcellular level, first providing a general overview and then focusing on nuclei and nuclear DNA content – by far the most frequent and most important application of flow cytometry in plant science. We review applications of the method from the early days to recent advances and discuss its applied aspects.


Flow Cytometry Genome Size Ploidy Level Intact Nucleus Genome Size Estimation 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.



We thank our colleagues Beáta Petrovská and Jana Čížková for sharing unpublished results and useful suggestions. This work was supported by the Czech Science Foundation (awards P501/10/1740, P501/10/1778 and P501/12/G090) and by the Ministry of Education, Youth and Sports of the Czech Republic and the European Regional Development Fund (Operational Programme Research and Development for Innovations No. ED0007/01/01.


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© Springer-Verlag Berlin Heidelberg 2014

Authors and Affiliations

  • Jan Vrána
    • 1
  • Petr Cápal
    • 1
  • Martina Bednářová
    • 1
  • Jaroslav Doležel
    • 1
  1. 1.Centre of the Region Haná for Biotechnological and Agricultural ResearchInstitute of Experimental BotanyOlomoucCzech Republic

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