Laser Assisted Bio-printing (LAB) of Cells and Bio-materials Based on Laser Induced Forward Transfer (LIFT)
Laser assisted bio-printing (LAB) is an emerging and complementary technology in the field of tissue engineering envisaging biomimetics applications. LAB allows to print cells and liquid materials with a cell-level resolution, which is comparable to the complex histology of living tissues. By giving tissue engineers control on cell density and organization, LAB potentially holds promise to fabricate living tissues with biomimetic physiological functionality. In this chapter, the physical parameters related to laser induced forward transfer (LIFT) technique, which is implemented in the LAB, are presented. These parameters, such as laser pulse energy and bio-ink viscosity are critical to control the cell printing process. They must be tuned according to each other in order to print viable cell patterns with respect to cell-level histological organization. Processing time is a concern when addressing tissue engineering involving living material like cells. Therefore, concerns regarding the design and technical implementation of LAB based rapid prototyping workstation are discussed. Experimental requirements are described in order to fabricate tissues using LAB. Some typical multi-component printing, 3D printing approaches and bio-printing in vivo are presented.
KeywordsTissue Engineering Droplet Size Human Umbilical Vein Endothelial Cell Sodium Alginate Tissue Construct
We acknowledge financial support from GIS-AMA (Advanced Materials in Aquitaine), ANR (Agence Nationale pour la Recherche), and Région Aquitaine.
- 5.Guillemot F, Mironov V, Nakamura M (2010) Bioprinting is coming of age. In: Report from the international conference on bioprinting and biofabrication in Bordeaux (3B’09). Biofabrication 2:010201. doi: 10.1088/1758-5082/2/1/010201
- 11.Brisbane (1971) Pattern Deposit by Laser-Google PatentsGoogle Scholar
- 13.Bohandy J, Kim BF, Adrian FJ, (Aug1986) Metal deposition from a supported metal film using an excimer laser. J Appl Phys 60:1538–1539Google Scholar
- 22.Wu PK, Ringeisen BR (2010) Development of human umbilical vein endothelial cell (HUVEC) and human umbilical vein smooth muscle cell (HUVSMC) branch/stem structures on hydrogel layers via biological laser printing (BioLP). Biofabrication 2:014111. doi: 10.1088/1758-5082/2/1/014111 ADSCrossRefGoogle Scholar
- 23.Gaebel R, Ma N, Liu J et al (2011) Patterning human stem cells and endothelial cells with laser printing for cardiac regeneration. Biomaterials 32(35):9218–9230Google Scholar
- 24.Koch L, Kuhn S, Sorg H et al (2009) Laser printing of skin cells and human stem cells. Tissue Eng Part C Methods 091221133515000: doi: 10.1089/ten.tec.2009.0397
- 26.Gruene M, Pflaum M, Hess C et al (2011) Laser printing of three-dimensional multicellular arrays for studies of cell-cell and cell-environment interactions. Tissue Eng Part C Methods 110629135038006: doi: 10.1089/ten.tec.2011.0185