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Characterization of Bacterial Community of “Hetao” Strong-Flavor Chinese Liquor Daqus by PCR–SSCP

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Proceedings of the 2012 International Conference on Applied Biotechnology (ICAB 2012)

Part of the book series: Lecture Notes in Electrical Engineering ((LNEE,volume 249))

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Abstract

“Daqu” is a fermentation starter that is used to initiate fermentations for the production of Chinese liquor. The total DNA extracted from Daqu samples were used as a template for PCR with universal primers of 16S rRNA. The amplicons were analyzed using single-strand conformational polymorphism (SSCP). It was observed that the bacterial SSCP profile indicated high diversity. The results showed that Leuconostoc citreum, Weissella cibaria, Acetobacter pasteurianus, and Lactobacillus mindensis were the most dominant species. Based on the SSCP analysis, a few differences in community structure were found between Daqu samples.

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Authors and Affiliations

  1. College of Life Science, Inner Mongolia University, Huhhot, 010021, People’s Republic of China

    Na Hai & Lin Yuan

Authors
  1. Na Hai
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  2. Lin Yuan
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Corresponding author

Correspondence to Lin Yuan .

Editor information

Editors and Affiliations

  1. College of Bioengineering, Tianjin University of Science and Technology, Tianjin, People's Republic of China

    Tong-Cun Zhang

  2. Nanjing University of Technology, Nanjing, People's Republic of China

    Pingkai Ouyang

  3. Department of Microbiology & Molecular Genetics, University of Texas Houston Medical School, Texas, Texas, USA

    Samuel Kaplan

  4. Wellcome Trust Sanger Institute, Cambridge, United Kingdom

    Bill Skarnes

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Hai, N., Yuan, L. (2014). Characterization of Bacterial Community of “Hetao” Strong-Flavor Chinese Liquor Daqus by PCR–SSCP. In: Zhang, TC., Ouyang, P., Kaplan, S., Skarnes, B. (eds) Proceedings of the 2012 International Conference on Applied Biotechnology (ICAB 2012). Lecture Notes in Electrical Engineering, vol 249. Springer, Berlin, Heidelberg. https://doi.org/10.1007/978-3-642-37916-1_52

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  • DOI: https://doi.org/10.1007/978-3-642-37916-1_52

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  • Publisher Name: Springer, Berlin, Heidelberg

  • Print ISBN: 978-3-642-37915-4

  • Online ISBN: 978-3-642-37916-1

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