Phase Contrast Image Restoration via Dictionary Representation of Diffraction Patterns
The restoration of microscopy images makes the segmentation and detection of cells easier and more reliable, which facilitates automated cell tracking and cell behavior analysis. In this paper, the authors analyze the image formation process of phase contrast images and propose an image restoration method based on the dictionary representation of diffraction patterns. By formulating and solving a min-ℓ1 optimization problem, each pixel is restored into a feature vector corresponding to the dictionary representation. Cells in the images are then segmented by the feature vector clustering. In addition to segmentation, since the feature vectors capture the information on the phase retardation caused by cells, they can be used for cell stage classification between intermitotic and mitotic/apoptotic stages. Experiments on three image sequences demonstrate that the dictionary-based restoration method can restore phase contrast images containing cells with different optical natures and provide promising results on cell stage classification.
KeywordsFeature Vector Phase Retardation Phase Contrast Image Cell Segmentation Bright Cell
- 1.Murphy, D.: Phase Contrast Microscopy and Dark-Field Micorscopy. In: Fundamentals of Light Microscopy and Electronic Imaging, pp. 97–116. Wiley (2001)Google Scholar
- 4.Kachouie, N.N., Fieguth, P., Jervis, E.: Watershed deconvolution for cell segmentation. In: 30th Annual International Conference of the IEEE Engineering in Medicine and Biology Society, pp. 375–378 (2008)Google Scholar