Microfluidic Device with Integrated Porous Membrane for Cell Sorting and Separation
In the real biological samples detection, the cells are mostly not from one single spice. In order to obtain the single spices for testing, and get the accurate biological, the requirement for sorting and separation different types of cells from complex samples needs to be met. Several approaches were developed to make miniaturized particle-sorting devices on a microfluidic platform. Dielectrophoretic forces , optical tweezing forces , hydrodynamic/hydrophoretic forces , magnetic forces , shear-induced lift forces , and gravity-driven forces  are introduced for cell sorting. However, many of these approaches are complicated, expensive, and require additional steps to label the particles to be sorted. Furthermore, the sorting efficiency of some of these methods is insufficient for diagnostic and therapeutic applications, such as polymerase chain reaction (PCR) [7, 8] or early cancer detection by circulating tumor cells (CTCs) .
KeywordsMicrofluidic Device Porous Membrane Driving Pressure PDMS Membrane PDMS Layer