Regulatory Systems that Quantitatively Alter Two Anionic Lipids of Chloroplasts in Chlamydomonas Reinhardtii upon Sulfur-Starvation

  • Koichi Sugimoto
  • Mikio Tsuzuki
  • Norihiro SatoEmail author
Part of the Advanced Topics in Science and Technology in China book series (ATSTC)


Sulfoquinovosyl diacylglycerol (SQDG) and phosphatidylglycerol (PG) are commonly anionic membrane lipids of chloroplasts. Chlamydomonas reinhardtii starved for sulfur (S) degrades SQDG to ensure a sulfur-source, simultaneously elevating the PG content as if to compensate for the loss of SQDG. Similar compensation is observed even under normal growth conditions in a mutant (hf-2) of C. reinhardtii deficient in SQDG, which shows a higher content of PG than the wild type. We here investigated signaling mechanisms by which these two lipids alter in C. reinhardtii during S-starvation, with the use of two mutants (sac1 and sac3) defective in normal responses to ambient S-status, and hf-2. Compared with the wild type, both sac mutants were largely repressed in the induction of SQDG degradation, indicating involvement of SAC1 and SAC3 genes in the induction. On the other hand, the wild type increased the PG synthesis by 3.5-fold after S-starvation of 3 h whereas hf-2 has already been 2.8-fold higher in the PG synthesis than the wild type under S-replete conditions to maintain the level after the shift to S-sarved conditions. Therefore, in C. reinhardtii under S-starved conditions, the loss of SQDG seems to stimulate PG synthesis to up-regulate the PG content.


Chlamydomonas reinhardtii Phosphatidylglycerol Sulfur starvation Sulfoquinovosyl diacylglycerol Thylakoid membranes 


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  1. Davies JP, Yildiz FH, Grossman AR (1996) Sac1, a Putative Regulator that Is Critical for Survival of Chlamydomonas Reinhardtii during Sulfur Deprivation. The EMBO Journal 15: 2150–2159PubMedGoogle Scholar
  2. Davies JP, Yildiz FH, Grossman AR (1999) Sac3, an Snf1-Like Serine/Threonine Kinase that Positively and Negatively Regulates the Responses of Chlamydomonas to Sulfur Limitation. Plant Cell 11: 1179–1190PubMedGoogle Scholar
  3. Sato N, Tsuzuki M, Matsuda Y, Ehara T, Osafune T, Kawaguchi A (1994) Isolation and Characterization of Mutants Affected in Lipid Metabolism of Chlamydomonas Reinhardtii. Eur. J. Biochem. 230: 987–993Google Scholar
  4. Sato N, Hagio M, Wada H, Tsuzuk M (2000) Requirement of Phosphatidylglycerol for Photosynthetic Function in Thylakoid Membranes. Proc. Nat. Acad. Sci. USA 97: 10655–10660PubMedCrossRefGoogle Scholar
  5. Sato N (2004) Roles of the Acidic Lipids Sulfoquinovosyl Diacylglycerol and Phosphatidylglycerol in Photosynthesis: Their Specificity and Evolution. J. Plant Res. 117: 495–505PubMedCrossRefGoogle Scholar
  6. Sato N, Aoki M, Maru Y, Sonoike K, Minoda A, Tsuzuki M (2003) Involvement of Sulfoquinovosyl Diacylglycerol in the Structural Integrity and Heat-Tolerance of Photosystem II. Planta 217: 245–251PubMedGoogle Scholar
  7. Sato N, Wada H (2009) Lipid Biosynthesis and Its Regulation in Cyanobacteria. In: Wada H, Murata N (eds.), Lipids in Photosynthesis: Essential and Regulatory Functions. Springer, pp. 157–177Google Scholar
  8. Shimojima M, Ohta H, Nakamura Y (2009) Biosynthesis and Function of Chloroplast Lipids. In: Wada H, Murata N (eds.), Lipids in Photosynthesis: Essential and Regulatory Functions. Springer, pp. 35–55Google Scholar
  9. Sugimoto K, Midorikawa T, Tsuzuki M, Sato N (2008) Upregulation of PG Synthesis on Sulfur-Starvation for PS I in Chlamydomonas. Biochem. Biophys. Res. Commun. 369: 660–665PubMedCrossRefGoogle Scholar
  10. Sugimoto K, Tsuzuki M, Sato N (2009) Utilization of a Chloroplast Membrane Sulfolipid as a Major Internal Sulfur Source for Protein Synthesis in the Early Phase of Sulfur Starvation in Chlamydomonas Reinhardtii. FEBS Lett. 581: 4519–4522CrossRefGoogle Scholar
  11. Sugimoto K, Tsuzuki M, Sato N (2010) Regulation of Synthesis and Degradation of a Sulfolipid under Sulfur-Starved Conditions and Its Physiological Significance in Chlamydomonas Reinhardtii. New Phytol. 185: 676–686PubMedCrossRefGoogle Scholar
  12. Zhang Z, Shrager J, Jain M, Chang CW, Vallon O, Grossman AR (2004) Insights into the Survival of Chlamydomonas Reinhardtii during Sulfur Starvation Based on Microarray Analysis of Gene Expression. Eukaryotic Cell 3: 1331–1348PubMedCrossRefGoogle Scholar

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© Zhejiang University Press, Hangzhou and Springer-Verlag Berlin Heidelberg 2013

Authors and Affiliations

  1. 1.School of Life SciencesTokyo University of Pharmacy and Life SciencesJapan

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