Multielectrode Array: A New Approach to Plant Electrophysiology

  • Elisa Masi
  • Elisa Azzarello
  • Stefano MancusoEmail author


A number of recent technical advances allowed the ideation of the multielectrode array (MEA) technology, a valuable tool to record electrical activity with high information content both in the spatial and temporal dimensions. Microfabricated arrays, recording hardware and software for data acquisition and analysis, are now commercially available and enable continuous, stable recordings. Here, the MEA system and the different arrays available are reviewed with regard to their intrinsic characteristics and performances. Some interesting applications of the MEA approach in plants and in combination with other techniques (e.g. imaging) are mentioned. Due to the emerging demand for novel electrophysiological methods that allows automated recording from cells and tissues, it is expected that the MEA technology will become a widely accepted and used tool in the field of plant electrophysiology.


Electrical Activity Root Apex Spike Rate Recording Area Light Addressable Potentiometric Sensor 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.



Multielectrode array


Printed board circuit








Titanium nitride


Indium tin-doped oxide


Interelectrode distance


Perforated MEA


Perfusion ground plate


Flexible MEA




High-density MEA


Complementary metal–oxide–semiconductor


Light addressable potentiometric sensors


Action potential


Variation potential


  1. Berdondini L, van der Wal PD, Guenat O, de Rooij NF, Koudelka-Hep M, Seitz P, Kaufmann R, Metzler P, Blanc N, Rohr S (2004) High-density electrode array for imaging in vitro electrophysiological activity. Biosens Bioelectron 21:167–174CrossRefGoogle Scholar
  2. Boppart SA, Wheeler BC, Wallace CS (1992) A flexible perforated microelectrode array for extended neural recordings. IEEE Trans Biomed Eng 39:37–42PubMedCrossRefGoogle Scholar
  3. Bucher V, Brunner B, Leibrock C, Schubert M, Nisch W (2001) Electrical properties of a light-addressable microelectrode chip with high electrode density for extracellular stimulation and recording of excitable cells. Biosens Bioelectron 16(3):205–210PubMedCrossRefGoogle Scholar
  4. Egert U, Knott T, Schwarz C, Nawrot M, Brandt A, Rotter S, Diesmann M (2002) MEA-tools: an open source toolbox for the analysis of multi-electrode data with MATLAB. J Neurosci Methods 117:33–42PubMedCrossRefGoogle Scholar
  5. Egert U, Schlosshauer B, Fennrich S, Nisch W, Fejtl M, Knott Th, Müller T, Hämmerle H (1998) A novel organotypic long-term culture of the rat hippocampus on substrate-integrated multielectrode arrays. Brain Res Protoc 2:229–242CrossRefGoogle Scholar
  6. Eversmann B, Jenkner M, Hofmann F, Paulus C, Brederlow R, Holzapfl B, Fromherz P, Merz M, Brenner M, Schreiter M, Gabl R, Plehnert K, Steinhauser M, Eckstein G, Schmitt-Landsiedel D, Thewes R (2003) A 128 × 128 CMOS biosensor array for extracellular recording of neural activity. IEEE J Solid-State Circuits 38(12):2306–2317CrossRefGoogle Scholar
  7. Eytan D, Minerbi A, Ziv NE, Marom S (2004) Dopamine-induced dispersion of correlations between action potentials in networks of cortical neurons. J Neurophysiol 92(3):1817–1824PubMedCrossRefGoogle Scholar
  8. Fejtl M, Stett A, Nisch W, Boven K-H, Möller A (2006) On micro-electrode array revival: its development, sophistication of recording, and stimulation. In: Taketani M, Baudry M (eds) Advances in network electrophysiology. Springer, BerlinGoogle Scholar
  9. Flickinger B, Berghofer T, Eing C, Gusbeth C, Strassner R, Frey W (2010) Transmembranepotential, easurements on plant cells using the voltage sensitive dye annine-6. Protoplasma 247:3–12PubMedCrossRefGoogle Scholar
  10. Frey U, Egert U, Heer F, Hafizovic S, Hierlemann A (2009) Microelectronic system for high-resolution mapping of extracellular electric fields applied to brain slices. Biosens Bioelectron 24:2191–2198PubMedCrossRefGoogle Scholar
  11. Fromherz P (2003) Neuroelectronic interfacing: semiconductor chips with ion channels, nerve cells, and brain. In: Waser J, Verlag W-VCH (eds) Nanoelectronics and information technology. Wiley VCH Publishing, BerlinGoogle Scholar
  12. Gross GW (1979) Simultaneous single unit recording in vitro with a photoetched laser deinsulated gold multimicroelectrode surface. IEEE Trans Biomed Eng 26:273–279PubMedCrossRefGoogle Scholar
  13. Gross GW, Rhoades BK, Reust DL, Schwahn FU (1993) Stimulation of monolayer networks in culture through thin-fihn indium-tin oxide recording electrodes. J Neurosci Methods 50(2):131–143PubMedCrossRefGoogle Scholar
  14. Halbach MD, Egert U, Hescheler J, Banach K (2003) Estimation of action potential changes from field potential recordings in multi-cellular mouse cardiac myocyte cultures. Cell Physiol Biochem 13:271–284PubMedCrossRefGoogle Scholar
  15. Heusckel MO, Fejl M, Raggenbass M, Bertrand D, Renaud P (2002) A three dimensional multi-electrode array for multi-site stimulation and recording in acute brain slices. J Neurosci Methods 114:135–148CrossRefGoogle Scholar
  16. Johnston D, Wu MS (1995) Extracellular field recordings. In: foundations of cellular neurophysiology. MIT Press, Cambridge, MAGoogle Scholar
  17. Kandler S, OkujeniS, Reinartz S, Egert U (2010) Networks in dissociated culture follow native corticaldevelopment. In: Stett A (ed) Proceedings MEA meeting 2010, Stuttgart: BIOPRO Baden-Württemberg GmbH 2010, pp 44–45Google Scholar
  18. Kerr JND, Denk W (2008) Imaging in vivo: watching the brain in action. Nat Rev Neurosci 9:195–203CrossRefGoogle Scholar
  19. Kovacs G (1994) Introduction to the theory, design and modeling of thin-film microelectrodes for neural interfaces. In: Stenger DA, McKenna T (eds) Enabling technologies for cultured neural networks. Academic, San DiegoGoogle Scholar
  20. Marrese CA (1987) Preparation of strongly adherent platinum black coatings. Anal Chem 59:217–218CrossRefGoogle Scholar
  21. Mancuso S (1999) Hydraulic and electrical transmission of wound-induced signals in Vitis vinifera. Aust J Plant Physiol 26:55–61CrossRefGoogle Scholar
  22. Masi E, Ciszak M, Stefano G, Renna L, Azzarello E, Pandolfi C, Mugnai S, Baluska F, Arecchi T, Mancuso S (2009) Spatiotemporal dynamics of the electrical network activity in the root apex. PNAS 106:4048–4053PubMedCrossRefGoogle Scholar
  23. Molina-Luna K, Buitrago MM, Hertler B, Schubring M, Haiss F, Nisch W, Schulz JB, Luft AR (2007) Cortical stimulation mapping using epidurally implanted thin-film microelectrode arrays. J Neurosci Methods 161(1):118–125PubMedCrossRefGoogle Scholar
  24. Pine J (1980) Recording action potentials from cultured neurons with extracellular microcircuit electrodes. J Neunxci Methods 2:19–31CrossRefGoogle Scholar
  25. Potter SM (2001) Distributed processing in cultured neuronal networks. Prog Brain Res 130:49–62PubMedCrossRefGoogle Scholar
  26. Regehr WG, Pine J, Cohan CS, Mischke MD, Tank DW (1989) Sealing cultured invertebrate neurons to embedded dish electrodes facilitates long-term stimulation and recording. J Neurosci Methods 30(2):91–106PubMedCrossRefGoogle Scholar
  27. Stett A, Barth W, Weiss S, Haemmerle H, Zrenner E (2000) Electrical multisite stimulation of the isolated chicken retina. Vis Res 40:1785–1795PubMedCrossRefGoogle Scholar
  28. Stett A, Egert U, Guenther E, Hofmann F, Meyer T, Nisch W, Haemmerle H (2003) Biological application of microelectrode arrays in drug discovery and basic research. Anal Bioanal Chem 377:486–495PubMedCrossRefGoogle Scholar
  29. Takayama Y, Moriguchi H, Jimbo Y (2008) Site-selective stimulation and recording of the electrical activity of cultured neuronal networks using mobile microelectrodes. In: Proceedings of the international symposium on biological and physiological engineering, pp 159–162Google Scholar
  30. Thomas CA, Springer PA, Loeb GE, Berwald-Netter Y, Okun LM (1972) A miniature microelectrode array to monitor the bioelectric activity of cultured cells. Exp Cell Res 74:61–66PubMedCrossRefGoogle Scholar
  31. Tsay C, Lacour SP, Wagner S, Morrison III B (2005) Architecture, fabrication, and properties of stretchable microelectrode arrays. In: Proceedings of the 4th IEEE conference on sensors, pp 1169–1172Google Scholar
  32. Volkov AG (2006) Plant electrophysiology: theory and methods. Springer, BerlinCrossRefGoogle Scholar

Copyright information

© Springer-Verlag Berlin Heidelberg 2012

Authors and Affiliations

  1. 1.Department of Plant, Soil and EnvironmentUniversity of FlorenceSesto FiorentinoItaly

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