Application of Non-invasive Microelectrode Flux Measurements in Plant Stress Physiology



Non-invasive microelectrode flux measurement (the MIFE™ technique) is a convenient tool to study membrane-transport processes in plants in situ. Over the last 20 years, many papers have been published elucidating the critical role of membrane-transport processes in response to a variety of abiotic and biotic stresses including salinity, osmotic stress, temperature extremes, acidity, oxygen deprivation, nutritional disorders, oxidative stress, and pathogens and elicitors. In this review, we summarize some of these findings and illustrate how the application of ion-selective microelectrodes may be combined with other techniques to address some fundamental issues related to mechanisms of plant nutrient acquisition and stress signaling and adaptation.



Abscisic acid-insensitive1


Arabidopsis K+ transporter1


Aluminum-activated malate transporter


Arbuscular mycorrhiza


Arabidopsis cyclic nucleotide-gated channel10


Arabidopsis DExD/H box RNA helicase


Adenosine triposphate


Cell death defective-9




Cytosolic free calcium


Membrane potential


Guard cell outward-rectifying K+ channel


Hypersensitive response


Viral-encoded K+ channel


K+ inward-rectifying channel


K+ outward-rectifying channel


Liquid ion exchanger


Multidrug and toxic efflux transporter


Marine bioactive substances


Nonspecific outward-rectifying channel


Non-selective cation channel




Pathogen-associated molecular patterns




Paramecium bursaria chlorella virus


Programmed cell death


Plasma membrane


Papaya mosaic virus


Potato virus X


Reactive oxygen species


Stretch-activated channels


Scanning ion-selective electrode technique




Terminal deoxynucleotidyl dUTP nick end labeling






Benzyloxycarbonyl-Val-Ala-Asp (OMe)—uoromethylketone


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© Springer-Verlag Berlin Heidelberg 2012

Authors and Affiliations

  1. 1.School of Agricultural ScienceUniversity of TasmaniaHobartAustralia

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