Application of Non-invasive Microelectrode Flux Measurements in Plant Stress Physiology

Chapter

Abstract

Non-invasive microelectrode flux measurement (the MIFE™ technique) is a convenient tool to study membrane-transport processes in plants in situ. Over the last 20 years, many papers have been published elucidating the critical role of membrane-transport processes in response to a variety of abiotic and biotic stresses including salinity, osmotic stress, temperature extremes, acidity, oxygen deprivation, nutritional disorders, oxidative stress, and pathogens and elicitors. In this review, we summarize some of these findings and illustrate how the application of ion-selective microelectrodes may be combined with other techniques to address some fundamental issues related to mechanisms of plant nutrient acquisition and stress signaling and adaptation.

Abbreviations

abi1

Abscisic acid-insensitive1

akt1

Arabidopsis K+ transporter1

ALMT

Aluminum-activated malate transporter

AM

Arbuscular mycorrhiza

AtCNGC10

Arabidopsis cyclic nucleotide-gated channel10

AtHELPS

Arabidopsis DExD/H box RNA helicase

ATP

Adenosine triposphate

CED-9

Cell death defective-9

Cu/a

Cu2+/ascorbate

[Ca2+]cyt

Cytosolic free calcium

Em

Membrane potential

GORK

Guard cell outward-rectifying K+ channel

HR

Hypersensitive response

Kcv

Viral-encoded K+ channel

KIR

K+ inward-rectifying channel

KOR

K+ outward-rectifying channel

LIX

Liquid ion exchanger

MATE

Multidrug and toxic efflux transporter

MBS

Marine bioactive substances

NORC

Nonspecific outward-rectifying channel

NSCC

Non-selective cation channel

O2

Oxygen

PAMPs

Pathogen-associated molecular patterns

PA

Polyamines

PBCV-1

Paramecium bursaria chlorella virus

PCD

Programmed cell death

PM

Plasma membrane

PMV

Papaya mosaic virus

PVX

Potato virus X

ROS

Reactive oxygen species

SAC

Stretch-activated channels

SIET

Scanning ion-selective electrode technique

TEA

Tetraethylammonium

TUNEL

Terminal deoxynucleotidyl dUTP nick end labeling

ucu2-2/gi2

ultracurvata2-2/gigantea-2

UV-C

Ultraviolet-C

zVAD-fmk

Benzyloxycarbonyl-Val-Ala-Asp (OMe)—uoromethylketone

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© Springer-Verlag Berlin Heidelberg 2012

Authors and Affiliations

  1. 1.School of Agricultural ScienceUniversity of TasmaniaHobartAustralia

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