Characterisation of Root Plasma Membrane Ca2+-Permeable Cation Channels: Techniques and Basic Concepts

Chapter

Abstract

Calcium is an important macronutrient, which is required for structural and regulatory needs. Elevation of the cytosolic free Ca2+ is widely accepted as a major signalling mechanism in plants, which encodes the information about a multitude of exogenous and endogenous stimuli. Ca2+ uptake by roots from the soil is a passive process, which is catalysed by specialised plasma membrane proteinaceous pores that are called cation channels. These channels demonstrate different biophysical and physiological characteristics and may belong to a number of gene families. This chapter summarises data on physiological techniques and basic concepts for investigation of Ca2+-permeable cation channels in plant root cells. The main focus of the chapter is on patch-clamp technique, ion-selective vibration microelectrodes and Ca2+-aequorin luminometry. In the past two decades, a combination of these techniques allowed to investigate Ca2+-permeable channels in higher plants and establish their crucial roles in plant cell physiology.

Keywords

Root Hair Cation Channel Pipette Solution Permeable Channel Electrophysiological Technique 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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Authors and Affiliations

  1. 1.Department of Physiology and Biochemistry of Plants Biological Faculty Belarusian State UniversityMinskBelarus

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