RNA Polymerases and Transcription Factors of Trypanosomes

Chapter
Part of the Nucleic Acids and Molecular Biology book series (NUCLEIC, volume 28)

Abstract

RNA synthesis in trypanosomatid organisms deviates substantially from what we see in model organisms. In these parasites, protein-coding genes are arranged in long tandem arrays that are polycistronically transcribed by an unresolved mode of transcription initiation. Moreover, the African Trypanosoma brucei has evolved a multifunctional RNA polymerase I system which it employs for pre-rRNA synthesis as well as for transcription of specific gene units encoding its major cell surface antigens. Additionally, the trypanosomatid RNA polymerase III system, by relying mostly on bidirectional tRNA gene promoters, exhibits clear differences to other eukaryotic systems. Interestingly, annotation of completed trypanosomatid genomes revealed only a small subset of basal transcription factors (BTFs), which suggested that trypanosomes have a simplified transcription machinery. Recent research, however, has demonstrated that trypanosomes possess extremely divergent orthologs of most BTFs and that these factors deviate in many aspects from their human counterparts; they are the focus of this chapter.

Keywords

Variant Surface Glycoprotein Tandem Affinity Purification Splice Leader snRNA Gene RRNA Promoter 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

Notes

Acknowledgments

I thank Tu N. Nguyen and Justin K. Kirkham for critical reading of the manuscript. This work was supported by National Institutes of Health grants AI059377 and AI073300 to A.G.

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© Springer-Verlag GmbH Berlin Heidelberg 2012

Authors and Affiliations

  1. 1.Department of Genetics and Developmental BiologyUniversity of Connecticut Health CenterFarmingtonUSA
  2. 2.Department of Molecular, Microbial and Structural BiologyUniversity of Connecticut Health CenterFarmingtonUSA

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