Abstract
Citrus production is seriously affected by HLB to which no resistant plant is available in the cultivated germplasm. Degenerate primers designed based on conserved NBS (nucleotide binding site) motifs contained in some plant resistance genes were used to isolate analogous sequences called resistance gene analogs (RGAs). About xx resistance gene analogs (RGAs) were cloned from several spices related to HLB using PCR method with primers above-mentioned. The BLASTN searches revealed that 43 DNA fragments showed different sequence homology with known plant R-genes or deposited RGAs and 12 sequences could be translated to polypeptides without stop codons. These RGAs and 12 deduced amino acid were analyzed by Clustalx and DNAMAN software. Sequence analysis showed these RGAs contain the conserved domains P-loop, Kinase-2a, Kinase-3a, and HD, which was conserved in NBS-LRR type disease resistance gene. These 12 RGAs shared 37.39%-43.43% identity with the resistance genes of TobaccoN, FlaxL6, Arabidopsis thaliana RPS2, RPS5, RPP8, RPM1. Gene duplication followed by sequence divergence were proposed as the mode for the evolution of a large number of distantly or closely related RGA genes in plants, and this mode may play a role in the generation of new resistance specificity and cloning related resistance genes about HLB.
This is a preview of subscription content, log in via an institution to check access.
Access this chapter
Tax calculation will be finalised at checkout
Purchases are for personal use only
Preview
Unable to display preview. Download preview PDF.
References
Dangl, J.L., Jones, J.D.G.: Plant pathogens and integrated defence responses to infection. Nature 411, 826–833 (2001)
Dixon, M.S., Hatzixanthis, K., Jones, D.A., Harrison, K., Jones, J.D.G.: The tomato Cf-5 disease resistance gene and six homologs show pronounced allelic variation in leucine-rich repeat copy number. Plant Cell 10, 1915–1925 (1998)
Anderson, P., Lawrence, G., Morrish, B., Aylife, M., Finnegan, E., Ellis, J.: Inactivation of the Xax rust resistance gene M associated with the loss of a repeated unit within the leucine-rich repeat coding region. Plant Cell 9, 641–651 (1997)
Belkhadir, Y., Subramaniam, R., Dangl, J.L.: Plant disease resistance protein signaling: NBS-LRR proteins and their partners. Curr. Opin. Plant Biol. 7, 391–399 (2004)
Zheng, X., Cui, W., Li, X.: NBS-LRR disease resistance analogues of Rice Varieties. Science in China 31(1), 43–51 (2001)
Gao, Y., Xu, Z., Jiao, F., Yu, H., Xiao, B., Li, Y., Lu, X.: Cloning, structural features, and expression analysis of resistance gene analogs in Tobacco. Mol. Biol. Rep. 37, 345–354 (2010)
Brugmans, B., Wouters, D., van Os, H., et al.: Genetic mapping and transcription analyses of resistance gene loci in potato using NBS profiling. Theor. Appl. Genet. 117, 1379–1388 (2008)
Meyers, B.C., Dickerman, A.W., Michelmore, R.W., Sivaramakrishnan, S., Sobral, B.W., Young, N.D.: Plant disease resistance genes encode members of an ancient and diverse protein family within the nucleotide-binding superfamily. Plant J. 20, 317–332 (1999)
Bove, J.M., Huang, L.: A destructive, newly-emerging, century-old disease of citrus. Plant Pathol. 88(1), 7–37 (2006)
Nariani, T.K.: Integrated approach to control citrus greening disease in India. Proc. Int. Soc. Citricult. 1, 471–472 (1981)
Miyakawa, T.: Experimentally-induced symptoms and host range of citrus likubin (greening disease). Annu. Phytopathol. Soc. Jpn. 46, 224–230 (1980)
Dirlewanger, E., Graziano, E., Joobeur, T., Garriga-Caldere, F., Cosson, P., Howad, W., Arus, P.: Comparative mapping and markerassisted selection in Rosaceae fruit crops. Proc. Natl. Acad. Sci. 101, 9891–9896 (2004)
Thompson, J.D., Gibson, T.J., Plewniak, F., Jeanmougin, F., Higgins, D.G.: The CLUSTAL_X windows interface: flexible strategies for multiple sequence alignment aided by quality analysis tools. Nucleic Acids Res. 25, 4876–4882 (1997)
Nicholas, K.B., Nicholas Jr., H.B., Deerfield II, D.W.: GeneDoc: analysis and visualization of genetic variation. EMBnet News 4, 14–17 (1997)
He, C.Y., Tian, A.G., Zhang, J.S., Zhang, Z.Y., Gai, J.Y., Chen, S.Y.: Isolation and characterization of a full-length resistance gene homolog from soybean. Theor. Appl. Genet. 106, 786–793 (2003)
Radwan, O., Bouzidi, M.F., Vear, F., Philippon, J., De Labrouhe, D.T., Nicolas, P., et al.: Identification of non-TIR-NBS-LRR markers linked to the Pl5/Pl8 locus for resistance to downy mildew in sunflower. Theor. Appl. Genet. 106, 1438–1446 (2003)
Yan, G.P., Chen, X.M., Line, R.: Resistance gene-analog polymorphism markers co-segregating with the Yr5 gene for resistance to wheat stripe rust. Theor. Appl. Genet. 106, 636–643 (2003)
Meyers, B.C., Dickerman, A.W., Michelmore, R.W., Sivaramakrishnan, S., Sobral, B.W., Young, N.D.: Plant disease resistance genes encode members of an ancient and diverse protein family within the nucleotide-binding superfamily. Plant J. 20, 317–332 (1999)
Shirasu, K., Schulze-Lefert, P.: Regulators of cell death in disease resistance. Plant Mol. Biol. 44, 371–385 (2003)
Author information
Authors and Affiliations
Corresponding author
Editor information
Editors and Affiliations
Rights and permissions
Copyright information
© 2012 Springer-Verlag GmbH Berlin Heidelberg
About this chapter
Cite this chapter
Tingting, L., Youping, Y., Yayu, L., Zhongkang, W. (2012). Isolation, Characterization and Sequence Analysis of Resistance Gene Analogs (RGAs) in Plants Related to Huanglongbing(HLB). In: Zhu, E., Sambath, S. (eds) Information Technology and Agricultural Engineering. Advances in Intelligent and Soft Computing, vol 134. Springer, Berlin, Heidelberg. https://doi.org/10.1007/978-3-642-27537-1_98
Download citation
DOI: https://doi.org/10.1007/978-3-642-27537-1_98
Publisher Name: Springer, Berlin, Heidelberg
Print ISBN: 978-3-642-27536-4
Online ISBN: 978-3-642-27537-1
eBook Packages: EngineeringEngineering (R0)