Molecular Cloning of Arabidopsis thaliana HsfA2 Gene and Agrobacterium-Mediated Genetic Transformation of Chrysanthemum morifolium Ramat
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Abstract
In this paper, the HsfA2 gene from Arabidopsis thaliana ecotype Columbia was firstly isolated by RT-PCR, the PCR fragment of HsfA2 gene was inserted into the binary vector pBI121 and got the resulted expression vector, which named pBI121-AtHsfA2. The vector was further transformed into the Agrobacterium tumefaciens LBA4404, and then the AtHsfA2 was transmitted into Chrysanthemum morifolium Ramat ‘Yurenmian’ by Agrobacterium-mediated method. Finally, four positive transgenic plants were obtained successfully after antibiotic selection, PCR and PCR-Southern detection. Our results provide scientific references for transgenic breeding of Chrysanthemum.
Keywords
Arabidopsis thaliana L. HsfA2 gene Genetic transformation Chrysanthemum morifolium RamatPreview
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