Advertisement

Polysaccharides from Acanthopanacis Senticosi: Isolation, Purification, Antioxidant and Immunological Activities In Vitro

  • Chen RuizhanEmail author
  • Yang Simin
  • Dong Hang
  • Liu Zhiqiang
Part of the Advances in Intelligent and Soft Computing book series (AINSC, volume 134)

Abstract

A water-soluble polysaccharide fraction, coded as ASP-2-1, were extracted from Acanthopanacis Senticosi with hot-water.They were isolated and purified by chromatography on EDAE-Sepharose Fast Flow Ion-exchange column and Sephadex G-75 gelpermeation column successively. Monosaccharide composition was determined by capillary zone electrophoresis (CZE), molecular weight and purity was determined by high-performance gelpermeation chromatography (HPGPC). The gel permeation chromatography (GPC) analysis showed that the average molecular weight (Mw) of polysaccharides were approximately 14573 Da which contained 89.47% carbohydrate, 7.45% uronic acid, 2.16% protein and six kinds of monosaccharides including glucose (Glu), arabinose (Ara), galactose (Gal), fructose (Fru), rhamnose (Rha), xylose (Xyl) in a molar ratio of 27.33:8.92:3.03:1:7.59:20.51. Furthermore, the immunobilological and antioxidant activities of ASP-2-1 were evaluated by MTT assay, ferric-reducing antioxidant power (FRAP) assay and the scavenging activities of ∙O2-,∙OH and DPPH, respectively. The ASP-2-1 could significantly promote the Spleen lymphocyte proliferation, pronounced higher reductive power, strong ∙OH radical scavenging activity, moderate ∙O2- and DPPH∙ radicals scavenging activities. It should be explored as a novel and potential natural antioxidant and immunostimulating agent for use in functional foods or medicine.

Keywords

Polysaccharides Acanthopanacis Senticosi Mon-osaccharides composition Antioxidant activity Immunological activity 

Preview

Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.

References

  1. 1.
    Cui, M., Sun, W.X., Song, F.R., et al.: Multi-stage mass spectrometric studies of triterpenoid saponins in crude extracts from Acanthopanax senticosus Harms. Rapid Communication in Mass Spectrometry 13, 873–879 (1999)CrossRefGoogle Scholar
  2. 2.
    Han, S.B., Yoon, Y.D., Ahn, H.J., et al.: Toll-like receptor-mediated activation of B cells and macrophages by polysaccharide isolated from cell culture of Acanthopanax senticosus. International Immunopharmacology 3, 1301–1312 (2003)CrossRefGoogle Scholar
  3. 3.
    Meng, Q.F., Yu, X.K., Xu, M.Y., et al.: Extraction of Acanthopanacis Senticosi Polysaccharides and Their Antioxidative Effect. Journal of Jilin University (Science Edition) 43(5), 683–686 (2005)Google Scholar
  4. 4.
    Chen, R.Z., Meng, F.L., Liu, Z.Q., et al.: Antitumor activities of different fractions of polysaccharide purified from Ornithogalum caudatum Ait. Carbohydrate Polymers 80, 845–851 (2010)CrossRefGoogle Scholar
  5. 5.
    Blumenkrantz, N., Asboe-Hansen, G.: New method for quantitative determination of uronic acids. Analytical Biochemistry 54, 484–489 (1973)CrossRefGoogle Scholar
  6. 6.
    Lowry, O.H., Rosebrough, N.J., Farr, A.L., et al.: Protein measurement with the folin phenol reagent. Journal of Biochemistry 193, 265–275 (1951)Google Scholar

Copyright information

© Springer-Verlag GmbH Berlin Heidelberg 2012

Authors and Affiliations

  • Chen Ruizhan
    • 1
    Email author
  • Yang Simin
    • 1
  • Dong Hang
    • 1
  • Liu Zhiqiang
    • 2
  1. 1.College of ChemistryChangchun Normal UniversityChangchunChina
  2. 2.Laboratory of New Drug ResearchChangchun Institute of Applied Chemistry, Chinese Academy of SciencesChangchunChina

Personalised recommendations