Polysaccharides from Acanthopanacis Senticosi: Isolation, Purification, Antioxidant and Immunological Activities In Vitro
A water-soluble polysaccharide fraction, coded as ASP-2-1, were extracted from Acanthopanacis Senticosi with hot-water.They were isolated and purified by chromatography on EDAE-Sepharose Fast Flow Ion-exchange column and Sephadex G-75 gelpermeation column successively. Monosaccharide composition was determined by capillary zone electrophoresis (CZE), molecular weight and purity was determined by high-performance gelpermeation chromatography (HPGPC). The gel permeation chromatography (GPC) analysis showed that the average molecular weight (Mw) of polysaccharides were approximately 14573 Da which contained 89.47% carbohydrate, 7.45% uronic acid, 2.16% protein and six kinds of monosaccharides including glucose (Glu), arabinose (Ara), galactose (Gal), fructose (Fru), rhamnose (Rha), xylose (Xyl) in a molar ratio of 27.33:8.92:3.03:1:7.59:20.51. Furthermore, the immunobilological and antioxidant activities of ASP-2-1 were evaluated by MTT assay, ferric-reducing antioxidant power (FRAP) assay and the scavenging activities of ∙O2-,∙OH and DPPH, respectively. The ASP-2-1 could significantly promote the Spleen lymphocyte proliferation, pronounced higher reductive power, strong ∙OH radical scavenging activity, moderate ∙O2- and DPPH∙ radicals scavenging activities. It should be explored as a novel and potential natural antioxidant and immunostimulating agent for use in functional foods or medicine.
KeywordsPolysaccharides Acanthopanacis Senticosi Mon-osaccharides composition Antioxidant activity Immunological activity
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